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Mouse Anti-AMIGO2 Recombinant Antibody (CBYY-C0756) (CBMAB-C2192-YY)

This product is mouse antibody that recognizes AMIGO2. The antibody CBYY-C0756 can be used for immunoassay techniques such as: ELISA, FC, IHC
See all AMIGO2 antibodies
Published Data
Mouse Anti-AMIGO2 Recombinant Antibody (CBYY-C0756) in IF
Representative image of A1 astrocytic marker AMIGO2. Scale bar = 40 μm. Quantification of the expression of AMIGO2 inside GFAP+ cells (n = 3). ...View More
Luchena, C., Zuazo-Ibarra, J., Valero, J., Matute, C., Alberdi, E., & Capetillo-Zarate, E. (2022). A neuron, microglia, and astrocyte triple co-culture model to study Alzheimer's disease. Frontiers in Aging Neuroscience, 14, 271.
Mouse Anti-AMIGO2 Recombinant Antibody (CBYY-C0756) in Immunoblot
Cell lysates were prepared from HepG2 cells transfected with human AMIGO1 (A1), AMIGO2 (A2), AMIGO3 (A3), and an empty vector (E). Immunoblotting with rTNK1A0012 (rTNK mAb). ...View More
Goto, K., Osaki, M., Izutsu, R., Tanaka, H., Sasaki, R., Tanio, A., ... & Okada, F. (2022). Establishment of an antibody specific for AMIGO2 improves immunohistochemical evaluation of liver metastases and clinical outcomes in patients with colorectal cancer. Diagnostic Pathology, 17(1), 16.
Mouse Anti-AMIGO2 Recombinant Antibody (CBYY-C0756) in WB
Western blotting of EMT related markers while AMIGO2 down-regulated in DU145 cell line (left panel). *, P<0.05. Relative expression of EMT-related markers in normal control and AMIGO2 down-regulated in DU145 cell line (right panel). ...View More
Han, Z., Feng, Y., Deng, Y., Tang, Z., Cai, S., Zhuo, Y., ... & Zhong, W. (2022). Integrated analysis reveals prognostic value and progression-related role of AMIGO2 in prostate cancer. Translational Andrology and Urology, 11(7), 914.
Mouse Anti-AMIGO2 Recombinant Antibody (CBYY-C0756) in WB
Western blot analysis of AMIGO2. (A) AMIGO2 expression in Caco-2 and HCT116 cells. (B) Transfection of EX-Mm13004-M02 increased AMIGO2 expression in Caco-2 cells. (C) Transfection of siRNA targeting AMIGO2 decreased AMIGO2 expression in HCT-116 cells. AMIGO2, adhesion molecule with Ig like domain 2; siRNA, small interfering RNA; OE, overexpression; NTC, non-targeting control. ...View More
Tanio, A., Saito, H., Amisaki, M., Hara, K., Sugezawa, K., Uejima, C., ... & Fujiwara, Y. (2021). AMIGO2 as a novel indicator of liver metastasis in patients with colorectal cancer. Oncology Letters, 21(4), 1-1.
Mouse Anti-AMIGO2 Recombinant Antibody (CBYY-C0756) in IHC
Reactive A1 astrocytes are reduced in male SOD1G93A mice by microglia Ager deletion. a Representative images of GFAP and AMIGO2 staining in the lumbar spinal cord ventral horn of the indicated mouse groups. b Quantification of GFAP+ area. c Quantification of GFAP+ and AMIGO2+ overlap area. Mean ± SD. N = 7 SOD1G93A Agerfl/fl Cx3cr1Cre/+ mice, and N = 9 SOD1G93A Ager+/+ Cx3cr1Cre/+ mice. Independent two sample two-sided t test. In b, *p = 0.0132. In c, **p = 0.0076. ...View More
MacLean, M., Juranek, J., Cuddapah, S., López-Díez, R., Ruiz, H. H., Hu, J., ... & Schmidt, A. M. (2021). Microglia RAGE exacerbates the progression of neurodegeneration within the SOD1G93A murine model of amyotrophic lateral sclerosis in a sex-dependent manner. Journal of neuroinflammation, 18(1), 1-24.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
CBYY-C0756
Antibody Isotype
IgA, κ
Application
WB, IP, IF, ELISA, IHC-P

Basic Information

Immunogen
Amino acids 142-171 near the N-terminus of AMIGO2 of human origin.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgA, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:30-1:3,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
IHC1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Adhesion Molecule With Ig Like Domain 2
Entrez Gene ID
347902
UniProt ID
Q86SJ2
Alternative Names
#N/A
Function
Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain. May be required for tumorigenesis of a subset of gastric adenocarcinomas.
Biological Process
Brain development Source: GO_Central
Heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: UniProtKB
Homophilic cell adhesion via plasma membrane adhesion molecules Source: UniProtKB
Negative regulation of apoptotic process Source: InterPro
Negative regulation of programmed cell death Source: UniProtKB
Positive regulation of synapse assembly Source: Ensembl
Cellular Location
Nucleus; Cell membrane. Associated with nucleus as well as plasma membrane. Restricted to somata of cerebellar as well as hippocampal neurons (By similarity).
Topology
Extracellular: 40-398 aa
Helical: 399-419 aa
Cytoplasmic: 420-522 aa
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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