Mouse Anti-AMIGO2 Recombinant Antibody (CBYY-C0756) (CBMAB-C2192-YY)

Name: Mouse Anti-AMIGO2 Recombinant Antibody (CBYY-C0756)
SKU: CBMAB-C2192-YY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AMIGO2 Recombinant Antibody (CBYY-C0756) in IF

Representative image of A1 astrocytic marker AMIGO2. Scale bar = 40 μm. Quantification of the expression of AMIGO2 inside GFAP+ cells (n = 3). ...View More

Luchena, C., Zuazo-Ibarra, J., Valero, J., Matute, C., Alberdi, E., & Capetillo-Zarate, E. (2022). A neuron, microglia, and astrocyte triple co-culture model to study Alzheimer's disease. Frontiers in Aging Neuroscience, 14, 271.

Mouse Anti-AMIGO2 Recombinant Antibody (CBYY-C0756) in Immunoblot

Cell lysates were prepared from HepG2 cells transfected with human AMIGO1 (A1), AMIGO2 (A2), AMIGO3 (A3), and an empty vector (E). Immunoblotting with rTNK1A0012 (rTNK mAb). ...View More

Goto, K., Osaki, M., Izutsu, R., Tanaka, H., Sasaki, R., Tanio, A., ... & Okada, F. (2022). Establishment of an antibody specific for AMIGO2 improves immunohistochemical evaluation of liver metastases and clinical outcomes in patients with colorectal cancer. Diagnostic Pathology, 17(1), 16.

Mouse Anti-AMIGO2 Recombinant Antibody (CBYY-C0756) in WB

Western blotting of EMT related markers while AMIGO2 down-regulated in DU145 cell line (left panel). *, P<0.05. Relative expression of EMT-related markers in normal control and AMIGO2 down-regulated in DU145 cell line (right panel). ...View More

Han, Z., Feng, Y., Deng, Y., Tang, Z., Cai, S., Zhuo, Y., ... & Zhong, W. (2022). Integrated analysis reveals prognostic value and progression-related role of AMIGO2 in prostate cancer. Translational Andrology and Urology, 11(7), 914.

Mouse Anti-AMIGO2 Recombinant Antibody (CBYY-C0756) in WB

Western blot analysis of AMIGO2. (A) AMIGO2 expression in Caco-2 and HCT116 cells. (B) Transfection of EX-Mm13004-M02 increased AMIGO2 expression in Caco-2 cells. (C) Transfection of siRNA targeting AMIGO2 decreased AMIGO2 expression in HCT-116 cells. AMIGO2, adhesion molecule with Ig like domain 2; siRNA, small interfering RNA; OE, overexpression; NTC, non-targeting control. ...View More

Tanio, A., Saito, H., Amisaki, M., Hara, K., Sugezawa, K., Uejima, C., ... & Fujiwara, Y. (2021). AMIGO2 as a novel indicator of liver metastasis in patients with colorectal cancer. Oncology Letters, 21(4), 1-1.

Mouse Anti-AMIGO2 Recombinant Antibody (CBYY-C0756) in IHC

Reactive A1 astrocytes are reduced in male SOD1G93A mice by microglia Ager deletion. a Representative images of GFAP and AMIGO2 staining in the lumbar spinal cord ventral horn of the indicated mouse groups. b Quantification of GFAP+ area. c Quantification of GFAP+ and AMIGO2+ overlap area. Mean ± SD. N = 7 SOD1G93A Agerfl/fl Cx3cr1Cre/+ mice, and N = 9 SOD1G93A Ager+/+ Cx3cr1Cre/+ mice. Independent two sample two-sided t test. In b, *p = 0.0132. In c, **p = 0.0076. ...View More

MacLean, M., Juranek, J., Cuddapah, S., López-Díez, R., Ruiz, H. H., Hu, J., ... & Schmidt, A. M. (2021). Microglia RAGE exacerbates the progression of neurodegeneration within the SOD1G93A murine model of amyotrophic lateral sclerosis in a sex-dependent manner. Journal of neuroinflammation, 18(1), 1-24.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-C0756

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 142-171 near the N-terminus of AMIGO2 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgA, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:30-1:3,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Adhesion Molecule With Ig Like Domain 2

Entrez Gene ID

347902

UniProt ID

Q86SJ2

Alternative Names

#N/A

Function

Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain. May be required for tumorigenesis of a subset of gastric adenocarcinomas.

Biological Process

Brain development Source: GO_Central
Heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: UniProtKB
Homophilic cell adhesion via plasma membrane adhesion molecules Source: UniProtKB
Negative regulation of apoptotic process Source: InterPro
Negative regulation of programmed cell death Source: UniProtKB
Positive regulation of synapse assembly Source: Ensembl

Cellular Location

Nucleus; Cell membrane. Associated with nucleus as well as plasma membrane. Restricted to somata of cerebellar as well as hippocampal neurons (By similarity).

Topology

Extracellular: 40-398 aa
Helical: 399-419 aa
Cytoplasmic: 420-522 aa

Liu, Y., Yang, J., Shi, Z., Tan, X., Jin, N., O'Brien, C., ... & Stack, M. S. (2021). In vivo selection of highly metastatic human ovarian cancer sublines reveals role for AMIGO2 in intra-peritoneal metastatic regulation. Cancer Letters, 503, 163-173.

Nakamura, S., Kanda, M., Shimizu, D., Tanaka, C., Inokawa, Y., Hattori, N., ... & Kodera, Y. (2020). AMIGO2 Expression as a Potential Prognostic Biomarker for Gastric Cancer. Anticancer Research, 40(12), 6713-6721.

Ye, W., Chen, C., Xu, Y., Lin, Z., Xinyi, H., Li, J., & Bai, P. (2020). AMIGO2 Promotes The Proliferation And Metastasis Of Nasopharyngeal Carcinoma Cells By Activating The MAPK Signaling Pathway. International Journal of Radiation Oncology, Biology, Physics, 108(3), e520.

Rengasamy Venugopalan, S., Farrow, E., Sanchez–Lara, P. A., Yen, S., Lypka, M., Jiang, S., & Allareddy, V. (2019). A novel nonsense substitution identified in the amigo 2 gene in an occulo‐auriculo‐vertebral spectrum patient. Orthodontics & craniofacial research, 22, 163-167.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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