Human Recombinant AMIGO2 protein, Biotin Conjugated, His Tag (V2LY-0526-LY2074)

Name: Human Recombinant AMIGO2 protein, Biotin Conjugated, His Tag
SKU: V2LY-0526-LY2074
Rating: 5 (1 reviews)

See all AMIGO2 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant AMIGO2 protein, Biotin Conjugated, His Tag consist of Amino Acid: 1-398 and predicts a molecular mass of 42.3 kDa.

Molecule Mass

42.3 kDa

Conjugates

Biotin

Sequence

Amino Acid: 1-398

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile Tris, NaCl, TCEP, Glycerol

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

Adhesion Molecule With Ig Like Domain 2

Function

Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain. May be required for tumorigenesis of a subset of gastric adenocarcinomas.

Biological Process

Brain development Source: GO_Central
Heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: UniProtKB
Homophilic cell adhesion via plasma membrane adhesion molecules Source: UniProtKB
Negative regulation of apoptotic process Source: InterPro
Negative regulation of programmed cell death Source: UniProtKB
Positive regulation of synapse assembly Source: Ensembl

Cellular Location

Nucleus; Cell membrane. Associated with nucleus as well as plasma membrane. Restricted to somata of cerebellar as well as hippocampal neurons (By similarity).

Topology

Extracellular: 40-398 aa
Helical: 399-419 aa
Cytoplasmic: 420-522 aa

Tanio, A., Saito, H., Amisaki, M., Hara, K., Sugezawa, K., Uejima, C., ... & Fujiwara, Y. (2021). AMIGO2 as a novel indicator of liver metastasis in patients with colorectal cancer. Oncology Letters, 21(4), 1-1.

Liu, Y., Yang, J., Shi, Z., Tan, X., Jin, N., O'Brien, C., ... & Stack, M. S. (2021). In vivo selection of highly metastatic human ovarian cancer sublines reveals role for AMIGO2 in intra-peritoneal metastatic regulation. Cancer Letters, 503, 163-173.

Nakamura, S., Kanda, M., Shimizu, D., Tanaka, C., Inokawa, Y., Hattori, N., ... & Kodera, Y. (2020). AMIGO2 Expression as a Potential Prognostic Biomarker for Gastric Cancer. Anticancer Research, 40(12), 6713-6721.

Ye, W., Chen, C., Xu, Y., Lin, Z., Xinyi, H., Li, J., & Bai, P. (2020). AMIGO2 Promotes The Proliferation And Metastasis Of Nasopharyngeal Carcinoma Cells By Activating The MAPK Signaling Pathway. International Journal of Radiation Oncology, Biology, Physics, 108(3), e520.

Rengasamy Venugopalan, S., Farrow, E., Sanchez–Lara, P. A., Yen, S., Lypka, M., Jiang, S., & Allareddy, V. (2019). A novel nonsense substitution identified in the amigo 2 gene in an occulo‐auriculo‐vertebral spectrum patient. Orthodontics & craniofacial research, 22, 163-167.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Human Recombinant AMIGO2 protein, Biotin Conjugated, His Tag?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant AMIGO2 protein, His Tag (CAT#: V2LY-0526-LY2073)

Related Products

Mouse Anti-AMIGO2 Recombinant Antibody (CBYY-C0756) (CAT#: CBMAB-C2192-YY)

Human Recombinant AMIGO2 protein, His Tag (CAT#: V2LY-0526-LY2073)

Mouse Anti-AMIGO2 Recombinant Antibody (273617) (CAT#: CBMAB-1073-CN)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry