Mouse Anti-CRTAM Recombinant Antibody (CBFYC-2235) (CBMAB-C2305-FY)

Name: Mouse Anti-CRTAM Recombinant Antibody (CBFYC-2235)
SKU: CBMAB-C2305-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CRTAM Recombinant Antibody (CBFYC-2235) (CBMAB-C2305-FY) in WB

Western immunoblot of proteins (25 µg/lane) from HLFs and HASMCs from 3 donors each or HMC-1 cells for CADM1 counter-receptors (shown on the right) with molecular markers (shown on the left). ...View More

Moiseeva, E. P., Roach, K. M., Leyland, M. L., & Bradding, P. (2013). CADM1 is a key receptor mediating human mast cell adhesion to human lung fibroblasts and airway smooth muscle cells. PLoS One, 8(4), e61579.

Distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBFYC-2235

Application

FC, WB

Immunogen

Recombinant human CRTAM Ser18-Ser286.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1 µg/ml
FC	2.5 µg/10^6 cells

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, Trehalose

Preservative

None

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Cytotoxic And Regulatory T Cell Molecule

Introduction

CRTAM (Cytotoxic And Regulatory T Cell Molecule) is a Protein Coding gene. Among its related pathways are Innate Immune System and Class I MHC mediated antigen processing and presentation. Gene Ontology (GO) annotations related to this gene include receptor binding. An important paralog of this gene is CADM1.

Entrez Gene ID

56253

UniProt ID

O95727

Alternative Names

Cytotoxic And Regulatory T Cell Molecule; Class-I MHC-Restricted T-Cell-Associated Molecule; Cytotoxic And Regulatory T-Cell Molecule; Class I MHC Restricted T Cell Associated Molecule; CD355 Antigen; CD355

Function

Mediates heterophilic cell-cell adhesion which regulates the activation, differentiation and tissue retention of various T-cell subsets (By similarity).

Interaction with CADM1 promotes natural killer (NK) cell cytotoxicity and IFNG/interferon-gamma secretion by CD8+ T-cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM1 in vivo (PubMed:15811952).

Regulates CD8+ T-cell proliferation in response to T-cell receptor (TCR) activation (By similarity).

Appears to be dispensable for CD8+ T-cell-mediated cytotoxicity (By similarity).

Interaction with SCRIB promotes the late phase of cellular polarization of a subset of CD4+ T-cells, which in turn regulates TCR-mediated proliferation and IFNG, IL17 and IL22 production (By similarity).

By interacting with CADM1 on CD8+ dendritic cells, regulates the retention of activated CD8+ T-cells within the draining lymph node (By similarity).

Required for the intestinal retention of intraepithelial CD4+ CD8+ T-cells and, to a lesser extent, intraepithelial and lamina propria CD8+ T-cells and CD4+ T-cells (By similarity).

Interaction with CADM1 promotes the adhesion to gut-associated CD103+ dendritic cells, which may facilitate the expression of gut-homing and adhesion molecules on T-cells and the conversion of CD4+ T-cells into CD4+ CD8+ T-cells (By similarity).

Biological Process

Adaptive immune response Source: UniProtKB-KW
Cell recognition Source: HGNC-UCL
Detection of stimulus Source: HGNC-UCL
Detection of tumor cell Source: HGNC-UCL
Establishment of T cell polarity Source: UniProtKB
Heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: UniProtKB
Lymphocyte migration into lymphoid organs Source: UniProtKB
Negative regulation of activated T cell proliferation Source: UniProtKB
Positive regulation of cytokine production Source: HGNC-UCL
Positive regulation of interferon-gamma production Source: UniProtKB
Positive regulation of natural killer cell mediated cytotoxicity Source: HGNC-UCL
Positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target Source: HGNC-UCL
Regulation of CD8-positive, alpha-beta T cell activation Source: UniProtKB
Regulation of immune response Source: Reactome
Regulation of T cell activation Source: UniProtKB
Regulation of T cell differentiation Source: UniProtKB

Cellular Location

Cell membrane. In a subset of CD4+ T-cells, colocalizes with SCRIB at the immunological synapse during the late phase of T-cell activation.

Topology

Extracellular: 18-287
Helical: 288-308
Cytoplasmic: 309-393

Phetsouphanh, C., Pillai, S., & Zaunders, J. J. (2017). Cytotoxic CD4+ T cells in viral infections. Frontiers in Immunology, 8, 1729.

Takeuchi, A., & Saito, T. (2017). CD4 CTL, a cytotoxic subset of CD4+ T cells, their differentiation and function. Frontiers in immunology, 8, 194.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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