Sign in or Register   Sign in or Register
  |  

Mouse Anti-NCBP2 Recombinant Antibody (3A12) (CBMAB-N1378-WJ)

This product is a Mouse antibody that recognizes NCBP2. The antibody 3A12 can be used for immunoassay techniques such as: ELISA.
See all NCBP2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
3A12
Antibody Isotype
IgG1, κ
Application
ELISA

Basic Information

Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
nuclear cap binding protein subunit 2, 20kDa
Introduction
The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Entrez Gene ID
22916
UniProt ID
P52298
Alternative Names
Nuclear Cap Binding Protein Subunit 2; Nuclear Cap Binding Protein Subunit 2, 20kDa; Nuclear Cap Binding Protein Subunit 2, 20kD; Cell Proliferation-Inducing Gene 55 Protein; 20 KDa Nuclear Cap-Binding Protein; NCBP 20 KDa Subunit; CBP20;
Function
Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus via its interaction with ALYREF/THOC4/ALY, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC) via its interaction with UPF1, promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2, thereby being required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP2/CBP20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires NCBP1/CBP80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. The conventional cap-binding complex with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus (PubMed:26382858).
Biological Process
Alternative mRNA splicing, via spliceosome Source: ComplexPortal
Cap-dependent translational initiation Source: ComplexPortal
Gene silencing by miRNA Source: ComplexPortal
Histone mRNA metabolic process Source: ComplexPortal
mRNA 3'-end processing Source: ComplexPortal
mRNA cis splicing, via spliceosome Source: UniProtKB
mRNA export from nucleus Source: ComplexPortal
mRNA splicing, via spliceosome Source: GO_Central
mRNA transcription by RNA polymerase II Source: ComplexPortal
Nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB
Positive regulation of mRNA 3'-end processing Source: UniProtKB
Positive regulation of phosphorylation of RNA polymerase II C-terminal domain Source: ComplexPortal
Positive regulation of RNA export from nucleus Source: UniProtKB
Positive regulation of transcription elongation from RNA polymerase II promoter Source: ComplexPortal
Pre-mRNA cleavage required for polyadenylation Source: UniProtKB
Primary miRNA processing Source: ComplexPortal
Regulation of translational initiation Source: UniProtKB
RNA interference Source: ComplexPortal
RNA splicing Source: UniProtKB
snRNA export from nucleus Source: UniProtKB
Cellular Location
Nucleus
Cytoplasm

Ling, L., Wen, Y., Xiong, Y., Liu, X., Chen, J., Liu, T., & Zhang, B. (2024). Anisomycin inhibits the activity of human ovarian cancer stem cells via regulating antisense RNA NCBP2‐AS2/MEK/ERK/STAT3 signaling. The Journal of Gene Medicine, 26(1), e3571.

Xie, J., Mo, T., Li, R., Zhang, H., Liang, G., Ma, T., ... & Pan, W. (2023). The m7G Reader NCBP2 Promotes Pancreatic Cancer Progression by Upregulating MAPK/ERK Signaling. Cancers, 15(22), 5454.

Arora, R., Haynes, L., Kumar, M., McNeil, R., Ashkani, J., Nakoneshny, S. C., ... & Bose, P. (2023). NCBP2 and TFRC are novel prognostic biomarkers in oral squamous cell carcinoma. Cancer Gene Therapy, 1-14.

Xu, X., Zhao, Y., Ying, Y., Zhu, H., Luo, J., Mou, T., & Zhang, Z. (2023). m7G‐related genes—NCBP2 and EIF4E3 determine immune contexture in head and neck squamous cell carcinoma by regulating CCL4/CCL5 expression. Molecular Carcinogenesis.

Bu, H., Cao, T., Li, X., Guo, Y., Guo, J., Wang, Y., ... & Wang, D. (2022). Diagnostic and prognostic potential of the novel biomarker nuclear cap binding protein subunit 2 (NCBP2) in colon adenocarcinoma. Journal of Gastrointestinal Oncology, 13(4), 1782.

Singh, M. D., Jensen, M., Lasser, M., Huber, E., Yusuff, T., Pizzo, L., ... & Girirajan, S. (2020). NCBP2 modulates neurodevelopmental defects of the 3q29 deletion in Drosophila and Xenopus laevis models. PLoS genetics, 16(2), e1008590.

Kugeratski, F. G., Atkinson, S. J., Neilson, L. J., Lilla, S., Knight, J. R., Serneels, J., ... & Zanivan, S. (2019). Hypoxic cancer–associated fibroblasts increase NCBP2-AS2/HIAR to promote endothelial sprouting through enhanced VEGF signaling. Science signaling, 12(567), eaan8247.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-NCBP2 Recombinant Antibody (3A12)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare