RAB13

This gene is a member of the Rab family of small G proteins and plays a role in regulating membrane trafficking between trans-Golgi network (TGN) and recycling endosomes (RE). The encoded protein is involved in the assembly of tight junctions, which are components of the apical junctional complex (AJC) of epithelial cells. The AJC plays a role in forming a barrier between luminal contents and the underlying tissue. Additional functions associated with the protein include endocytic recycling of occludin, regulation of epithelial cell scattering, neuronal regeneration and regulation of neurite outgrowth. Alternately spliced transcript variants have been observed for this gene. A pseudogene associated with this gene is located on chromosome 12. [provided by RefSeq, Jan 2013]

Full Name

RAB13, member RAS oncogene family

Function

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis.

Biological Process

Biological Process bicellular tight junction assemblyManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process cellular response to insulin stimulusISS:UniProtKB
Biological Process cortical actin cytoskeleton organizationISS:UniProtKB
Biological Process endocytic recyclingManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process endosomal transportManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process endothelial cell chemotaxisISS:UniProtKB
Biological Process establishment of Sertoli cell barrierISS:UniProtKB
Biological Process Golgi vesicle fusion to target membraneManual Assertion Based On ExperimentIBA:GO_Central
Biological Process neuron projection developmentManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein kinase A signalingManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein localization to cell leading edgeIEA:Ensembl
Biological Process protein localization to plasma membraneManual Assertion Based On ExperimentIMP:UniProtKB
Biological Process protein secretionManual Assertion Based On ExperimentIBA:GO_Central
Biological Process regulation of exocytosisManual Assertion Based On ExperimentIBA:GO_Central
Biological Process trans-Golgi network to recycling endosome transportISS:UniProtKB
Biological Process vesicle docking involved in exocytosisManual Assertion Based On ExperimentIBA:GO_Central

Cellular Location

Cell membrane
Cytoplasmic vesicle membrane
Cell junction, tight junction
Golgi apparatus, trans-Golgi network membrane
Recycling endosome membrane
Cell projection, lamellipodium
Tight junctions or associated with vesicles scattered throughout the cytoplasm in cells lacking tight junctions (PubMed:8294494).
Relocalizes to the leading edge of lamellipodia in migrating endothelial cells (By similarity).

PTM

(Microbial infection) Stearoylated By S.flexneri N-epsilon-fatty acyltransferase IcsB, thereby disrupting the host actin cytoskeleton.