Mouse Anti-BRD3 Recombinant Antibody (CBYY-0801) (CBMAB-0804-YY)

Name: Mouse Anti-BRD3 Recombinant Antibody (CBYY-0801)
SKU: CBMAB-0804-YY
Rating: 5 (1 reviews)

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Published Data

CBMAB-0804-YY in WB

RD3 was detected by western blotting at different time points after ZXH-3-26 treatment at 100 nM concentration. ...View More

Shi, Y., Liao, Y., Liu, Q., Ni, Z., Zhang, Z., Shi, M., ... & Rao, Y. (2023). BRD4-targeting PROTAC as a unique tool to study biomolecular condensates. Cell Discovery, 9(1), 47.

CBMAB-0804-YY in WB and IF

SHH-GFP cells were treated with 1 μM of HPP-9 or dBet6 for 27 h and probed for GLI1, BRD2, BRD3, and BRD4 by western blot (a) and analyzed by fluorescence microscopy (b, c). ...View More

Bagka, M., Choi, H., Héritier, M., Schwaemmle, H., Pasquer, Q. T., Braun, S. M., ... & Hoogendoorn, S. (2023). Targeted protein degradation reveals BET bromodomains as the cellular target of Hedgehog Pathway Inhibitor-1. Nature Communications, 14(1), 3893.

CBMAB-0804-YY in Immunoblot

BRD proteins, MYC and pSTAT3 immunoblotting after birabresib or MZ1 treatment. Four ABC-DLBCL cell lines were treated with 500 nmol/L of DMSO, birabresib, MZ1 or cisMZ1. ...View More

Tarantelli, C., Cannas, E., Ekeh, H., Moscatello, C., Gaudio, E., Cascione, L., ... & Bertoni, F. (2021). The bromodomain and extra-terminal domain degrader MZ1 exhibits preclinical anti-tumoral activity in diffuse large B-cell lymphoma of the activated B cell-like type. Exploration of Targeted Anti-tumor Therapy, 2(6), 586.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-0801

Application

WB, IP, IF, FC

Immunogen

C-terminal region of BRD3 of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.1 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Bromodomain Containing 3

Introduction

This gene was identified based on its homology to the gene encoding the RING3 protein, a serine/threonine kinase. The gene localizes to 9q34, a region which contains several major histocompatibility complex (MHC) genes. The function of the encoded protein is not known.

Entrez Gene ID

8019

UniProt ID

Q15059

Alternative Names

Bromodomain Containing 3; RING3-Like Protein; RING3L; Bromodomain-Containing Protein 3 Short Isoform; Bromodomain-Containing Protein 3; Bromodomain-Containing 3; RING3-Like; KIAA0043; ORFX;

Function

Chromatin reader that recognizes and binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors (PubMed:18406326, PubMed:27105114).
Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity).

Biological Process

Chromatin organization Source: UniProtKB-KW
Regulation of transcription by RNA polymerase II Source: UniProtKB

Cellular Location

Nucleus. Detected on chromatin.

Involvement in disease

A chromosomal aberration involving BRD3 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;9)(q14;q34) with NUTM1 which produces a BRD3-NUTM1 fusion protein.

Wu, L., & Qi, Z. (2021). Long non-coding RNA NEAT1 deteriorates polycystic ovary syndrome progression via binding microRNA-324-3p to target bromodomain containing 3.

Daneshvar, K., Ardehali, M. B., Klein, I. A., Hsieh, F. K., Kratkiewicz, A. J., Mahpour, A., ... & Mullen, A. C. (2020). lncRNA DIGIT and BRD3 protein form phase-separated condensates to regulate endoderm differentiation. Nature cell biology, 22(10), 1211-1222.

Ren, W., Wang, C., Wang, Q., Zhao, D., Zhao, K., Sun, D., ... & Li, N. (2017). Bromodomain protein Brd3 promotes Ifnb1 transcription via enhancing IRF3/p300 complex formation and recruitment to Ifnb1 promoter in macrophages. Scientific reports, 7(1), 1-10.

Roberts, T. C., Etxaniz, U., Dall’Agnese, A., Wu, S. Y., Chiang, C. M., Brennan, P. E., ... & Puri, P. L. (2017). BRD3 and BRD4 BET bromodomain proteins differentially regulate skeletal myogenesis. Scientific reports, 7(1), 1-16.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

BRD3 Matched Antibody Pair (143) (CAT#: APMAB-143LY)

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BRD3 Matched Antibody Pair (143) (CAT#: APMAB-143LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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