Mouse Anti-ADV Recombinant Antibody (V2-503423) (CBMAB-V208-1364-FY)
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Infectivity assay on a A549 (high-CAR) and b SKOV-3 (low-CAR) cell lines. Both the cell lines were infected with ExtraCRAd (red) or the naked virus (blue). Cell viability at 3 days was evaluated by the MTS assay. The data are presented as mean ± s.d. (n = 3). c In vivo assessment of the oncolytic efficacy of the naked virus compared to ExtraCRAd in A549 xenografts in nude mice. The mice were injected subcutaneously with 5 × 106 cells per flank. The treatments (mock, PBS; CCM, cell membrane vesicles derived from 3 × 106 A549 cells; Virus, 1 × 109 viral particles; ExtraCRAd, 1 × 109 viral particles co-extruded with 3 × 106 A549 cells) were injected at day 15 and at day 25 post tumor implantation. The data are presented as mean ± SEM (n ≥ 8) and were analyzed by two-way ANOVA followed by Tukey's post-test. Error bars represents SEM. The levels of statistical significance were set at probabilities **p < 0.01 and ***p < 0.001. d Uptake kinetic study: the differences in the uptake kinetic were assessed by infecting A549 cells with ExtraCRAd or naked virus (adenovirus modified to express luciferase) for 1, 2, or 3 h, and by 24 h incubation to allow the expression of luciferase. The data are presented as mean ± s.d. (n ≥ 3) with the dot plot of the single replicates. Unpaired t-test was used to assess statistical significance. Neutralizing antibodies assay performed with e plasma from pre-immunized mice and f with anti-adenovirus serotype 5 antibody. ExtraCRAd and the naked virus were pre-incubated with the serum of pre-immunized mice at different dilutions (e) or with the antibody diluted 1:2 000 (f) for 60 min before the infection. A549 cells were infected with ExtraCRAd or the naked virus for 2 h, followed by 24 h of incubation, before being lysed. The data are presented as mean ± s.d. (n ≥ 3). Analysis with a two-way ANOVA, followed by Fisher LSD post-test was performed in f, while a two-way ANOVA, followed by Sidak post-test, was used to assess statistical significance in all the experiments, unless otherwise specified. Levels of significance were set at *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. Error bars represent s.d., unless otherwise specified. ...View More
Basic Information
| Application | Note |
| ELISA | 1:100-1:2,000 |
| IHC | 1:10-1:500 |
Formulations & Storage [For reference only, actual COA shall prevail!]
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Please try the standard protocols which include: protocols, troubleshooting and guide.
Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols
Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
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