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RPN1

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq]

Full Name

ribophorin I

Function

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc3Man9GlcNAc2 in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:31831667).
N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

Biological Process

Biological Process protein modification processManual Assertion Based On ExperimentTAS:ProtInc
Biological Process protein N-linked glycosylationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein N-linked glycosylation via asparagineManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Endoplasmic reticulum
Endoplasmic reticulum membrane
Melanosome
Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Topology

Lumenal: 24-438
Helical: 439-457
Cytoplasmic: 458-607

PTM

Ufmylated by UFL1 in response to endoplasmic reticulum stress, promoting reticulophagy of endoplasmic reticulum sheets.

Anti-RPN1 antibodies

Mouse Anti-RPN1 Recombinant Antibody (1G5) (CBMAB-R3407-CN)

SDS

Target: RPN1

Host: Mouse

Antibody Isotype: IgG2a

Specificity: Human

Clone: 1G5

Application*: WB, IF, F

Mouse Anti-RPN1 Recombinant Antibody (2C10-2F6) (CBMAB-R3408-CN)

SDS

Target: RPN1

Host: Mouse

Antibody Isotype: IgG1, κ

Specificity: Human

Clone: 2C10-2F6

Application*: E, WB

Mouse Anti-RPN1 Recombinant Antibody (2E3) (CBMAB-R3409-CN)

SDS

Target: RPN1

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human, Dog

Clone: 2E3

Application*: WB, IH, F

Mouse Anti-RPN1 Recombinant Antibody (2G1) (CBMAB-R3410-CN)

SDS

Target: RPN1

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human, Dog

Clone: 2G1

Application*: WB, IF, F

Mouse Anti-RPN1 Recombinant Antibody (2G9) (CBMAB-R3411-CN)

SDS

Target: RPN1

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: 2G9

Application*: WB, F

Mouse Anti-RPN1 Recombinant Antibody (3E1) (CBMAB-R3412-CN)

SDS

Target: RPN1

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human, Rat, Monkey

Clone: 3E1

Application*: WB, IH, IF, F

Mouse Anti-RPN1 Recombinant Antibody (3H3) (CBMAB-R3413-CN)

SDS

Target: RPN1

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human, Dog, Monkey

Clone: 3H3

Application*: WB, IH, F

Mouse Anti-RPN1 Recombinant Antibody (5A1) (CBMAB-R3415-CN)

SDS

Target: RPN1

Host: Mouse

Antibody Isotype: IgG2a

Specificity: Human

Clone: 5A1

Application*: WB, IF, F

Mouse Anti-RPN1 Recombinant Antibody (5B1) (CBMAB-R3416-CN)

SDS

Target: RPN1

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: 5B1

Application*: WB, IH, IF, F

Mouse Anti-RPN1 Recombinant Antibody (6A9) (CBMAB-R3417-CN)

SDS

Target: RPN1

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human, Dog

Clone: 6A9

Application*: WB, IH, IF, F

Mouse Anti-RPN1 Recombinant Antibody (6F2) (CBMAB-R3418-CN)

SDS

Target: RPN1

Host: Mouse

Antibody Isotype: IgG2b

Specificity: Human

Clone: 6F2

Application*: WB, IF, F

Mouse Anti-RPN1 Recombinant Antibody (CBCNR-495) (CBMAB-R3421-CN)

SDS

Target: RPN1

Host: Mouse

Antibody Isotype: IgG1

Specificity: Human

Clone: CBCNR-495

Application*: WB

Mouse Anti-RPN1 Recombinant Antibody (CBCNR-729) (CBMAB-R3422-CN)

SDS

Target: RPN1

Host: Mouse

Specificity: Human

Clone: CBCNR-729

Application*: WB

Mouse Anti-RPN1 Recombinant Antibody (CBCNR-731) (CBMAB-R3424-CN)

SDS

Target: RPN1

Host: Mouse

Specificity: Human

Clone: CBCNR-731

Application*: WB

Mouse Anti-RPN1 Recombinant Antibody (CBCNR-732) (CBMAB-R3425-CN)

SDS

Target: RPN1

Host: Mouse

Specificity: Human

Clone: CBCNR-732

Application*: WB, ICC, P, C, E

For Research Use Only. Not For Clinical Use.

(P): Predicted

* Abbreviations

IFImmunofluorescence

IHImmunohistochemistry

IPImmunoprecipitation

WBWestern Blot

EELISA

MMicroarray

CIChromatin Immunoprecipitation

FFlow Cytometry

FNFunction Assay

IDImmunodiffusion

RRadioimmunoassay

TCTissue Culture

GSGel Supershift

NNeutralization

BBlocking

AActivation

IInhibition

DDepletion

ESELISpot

DBDot Blot

MCMass Cytometry/CyTOF

CTCytotoxicity

SStimulation

AGAgonist

APApoptosis

IMImmunomicroscopy

BABioassay

CSCostimulation

EMElectron Microscopy

IEImmunoelectrophoresis

PAPeptide Array

ICImmunocytochemistry

PEPeptide ELISA

MDMeDIP

SHIn situ hybridization

IAEnzyme Immunoassay

SEsandwich ELISA

PLProximity Ligation Assay

ECELISA(Cap)

EDELISA(Det)

BIBioimaging

IOImmunoassay

LFLateral Flow Immunoassay

LALuminex Assay

CImmunohistochemistry-Frozen Sections

PImmunohistologyp-Paraffin Sections

ISIntracellular Staining for Flow Cytometry

MSElectrophoretic Mobility Shift Assay

RIRNA Binding Protein Immunoprecipitation (RIP)

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