Mouse Anti-RPN1 Recombinant Antibody (1G5) (CBMAB-R3407-CN)

Name: Mouse Anti-RPN1 Recombinant Antibody (1G5)
SKU: CBMAB-R3407-CN
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

1G5

Application

WB, IF, FC

Immunogen

Full length human recombinant protein of human RPN1 (NP_002941) produced in HEK293T cell

Specificity

Human

Antibody Isotype

IgG2a

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH 7.3, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Concentration

1.1 mg/mL

More Infomation

Target

Full Name

ribophorin I

Introduction

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein forms part of the regulatory subunit of the 26S proteasome and may mediate binding of ubiquitin-like domains to this proteasome. [provided by RefSeq, Jul 2008]

Entrez Gene ID

6184

UniProt ID

P04843

Alternative Names

Ribophorin I; Oligosaccharyltransferase Complex Subunit (Non-Catalytic); Ribophorin-1; RPN-I; Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase 67 KDa Subunit; Dolichyl-Diphosphooligosaccharide-Protein Glycosyltransferase 67 KDa Subunit; Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit 1;

Function

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc3Man9GlcNAc2 in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:31831667).
N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

Biological Process

Biological Process protein modification processManual Assertion Based On ExperimentTAS:ProtInc
Biological Process protein N-linked glycosylationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein N-linked glycosylation via asparagineManual Assertion Based On ExperimentIMP:UniProtKB

Cellular Location

Endoplasmic reticulum
Endoplasmic reticulum membrane
Melanosome
Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Topology

Lumenal: 24-438
Helical: 439-457
Cytoplasmic: 458-607

PTM

Ufmylated by UFL1 in response to endoplasmic reticulum stress, promoting reticulophagy of endoplasmic reticulum sheets.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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