RPN2

This gene encodes a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Full Name

ribophorin II

Function

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc3Man9GlcNAc2 in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:31831667).
N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

Biological Process

Biological Process agingIEA:Ensembl
Biological Process protein modification processManual Assertion Based On ExperimentTAS:ProtInc
Biological Process protein N-linked glycosylationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein N-linked glycosylation via asparagine1 PublicationNAS:HGNC-UCL
Biological Process response to xenobiotic stimulusIEA:Ensembl

Cellular Location

Endoplasmic reticulum
Endoplasmic reticulum membrane

Topology

Lumenal: 23-540
Helical: 541-561
Cytoplasmic: 562-571
Helical: 572-592
Lumenal: 593-596
Helical: 597-617
Cytoplasmic: 618-631