Human Recombinant RPN2 protein, His Tag (V2LY-0526-LY6544)

Name: Human Recombinant RPN2 protein, His Tag
SKU: V2LY-0526-LY6544
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Expressed Host

HEK293 Cells

Protein Species

Human

Tag

His Tag

Protein Construction

This product is Human Recombinant RPN2 protein, His Tag consist of Amino Acid: 1-540 and predicts a molecular mass of 58.4 kDa.

Molecule Mass

58.4 kDa

Sequence

Amino Acid: 1-540

Species

Human

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Please contact us for more information.

Format

Lyophilized

Reconstitution

Allow the vial and reconstitution buffer to equilibrate to room temperature. Briefly centrifuge or tap down the vial to ensure that all lyophilized powder is collected at the bottom of the vial. For the reconstitution of this product, we recommend adding PBS or sterile water to achieve a final antibody concentration of 1 mg/mL. Allow the vial to reconstitute for 10-15 minutes at room temperature with gentle agitation. Avoid vigorous shaking that can cause foaming and antibody denaturation. Aliquot into volumes based on your experiment and store liquid protein at -20°C or -80°C for long time.

Buffer

Lyophilized from sterile PBS

Preservative

None

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

More Infomation

Target

Full Name

ribophorin II

Function

Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc3Man9GlcNAc2 in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:31831667).
N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.

Biological Process

Biological Process agingIEA:Ensembl
Biological Process protein modification processManual Assertion Based On ExperimentTAS:ProtInc
Biological Process protein N-linked glycosylationManual Assertion Based On ExperimentIDA:UniProtKB
Biological Process protein N-linked glycosylation via asparagine1 PublicationNAS:HGNC-UCL
Biological Process response to xenobiotic stimulusIEA:Ensembl

Cellular Location

Endoplasmic reticulum
Endoplasmic reticulum membrane

Topology

Lumenal: 23-540
Helical: 541-561
Cytoplasmic: 562-571
Helical: 572-592
Lumenal: 593-596
Helical: 597-617
Cytoplasmic: 618-631

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Human Recombinant RPN2 protein, hFc Tag (CAT#: V2LY-0526-LY6543)

Related Products

Mouse Anti-RPN2 Recombinant Antibody (1G9) (CAT#: CBMAB-R3427-CN)

Mouse Anti-RPN2 Recombinant Antibody (1121) (CAT#: CBMAB-R3426-CN)

Human Recombinant RPN2 protein, hFc Tag (CAT#: V2LY-0526-LY6543)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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