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TDP2

Tdp2 is a DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Tdp2 catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DNA double-strand breaks/DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Thereby, Tdp2 protects the transcription of many genes involved in neurological development and maintenance from the abortive activity of TOP2.
Full Name
TDP2
Function
DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DNA double-strand breaks/DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation (PubMed:27099339, PubMed:27060144).
Thereby, protects the transcription of many genes involved in neurological development and maintenance from the abortive activity of TOP2. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DSBs due to DNA damage by radiation and free radicals. Has preference for single-stranded DNA or duplex DNA with a 4 base pair overhang as substrate. Acts as a regulator of ribosome biogenesis following stress. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. Constitutes the major if not only 5'-tyrosyl-DNA phosphodiesterase in cells. Also acts as an adapter by participating in the specific activation of MAP3K7/TAK1 in response to TGF-beta: associates with components of the TGF-beta receptor-TRAF6-TAK1 signaling module and promotes their ubiquitination dependent complex formation. Involved in non-canonical TGF-beta induced signaling routes. May also act as a negative regulator of ETS1 and may inhibit NF-kappa-B activation.
(Microbial infection) Also acts as a 5'-tyrosyl-RNA phosphodiesterase following picornavirus infection: its activity is hijacked by picornavirus and acts by specifically cleaving the protein-RNA covalent linkage generated during the viral genomic RNA replication steps of a picornavirus infection, without impairing the integrity of viral RNA.
Biological Process
Biological Process cell surface receptor signaling pathwayTAS:ProtInc1 Publication
Biological Process double-strand break repairIDA:UniProtKB1 Publication
Biological Process neuron developmentIMP:UniProtKB1 Publication
Involvement in disease
Spinocerebellar ataxia, autosomal recessive, 23 (SCAR23):
A form of spinocerebellar ataxia, a clinically and genetically heterogeneous group of cerebellar disorders due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCAR23 patients manifest epilepsy, intellectual disability, and gait ataxia.
PTM
Ubiquitinated by TRAF6.

Anti-TDP2 antibodies

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Target: TDP2
Host: Mouse
Specificity: Human
Clone: CBFYE-1666
Application*: IC, IH, IP, WB, CI
Target: TDP2
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human
Clone: 3D11
Application*: WB, E
Target: TDP2
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 11BA43
Application*: E, WB
Target: TDP2
Host: Mouse
Antibody Isotype: IgG2a
Specificity: Human
Clone: 11BA44
Application*: E, WB
Target: TDP2
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: 11BA45
Application*: E, WB
Target: TDP2
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human
Clone: CBYJT-2455
Application*: E, P, WB
Target: TDP2
Host: Mouse
Antibody Isotype: IgG2b, κ
Specificity: Human
Clone: CBYJT-2456
Application*: P, IF, IC, F
Target: TDP2
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human
Clone: CBYJT-2457
Application*: E, WB
Target: TDP2
Host: Mouse
Antibody Isotype: IgG1
Specificity: Human, Mouse, Rat, Chicken, Frog
Clone: CBYJT-2458
Application*: WB, IC, IP
Target: Tdp2
Host: Mouse
Antibody Isotype: IgG
Specificity: Mouse
Clone: CBYJT-2459
Application*: WB, IP, IF, E
For Research Use Only. Not For Clinical Use.
(P): Predicted
* Abbreviations
IFImmunofluorescence
IHImmunohistochemistry
IPImmunoprecipitation
WBWestern Blot
EELISA
MMicroarray
CIChromatin Immunoprecipitation
FFlow Cytometry
FNFunction Assay
IDImmunodiffusion
RRadioimmunoassay
TCTissue Culture
GSGel Supershift
NNeutralization
BBlocking
AActivation
IInhibition
DDepletion
ESELISpot
DBDot Blot
MCMass Cytometry/CyTOF
CTCytotoxicity
SStimulation
AGAgonist
APApoptosis
IMImmunomicroscopy
BABioassay
CSCostimulation
EMElectron Microscopy
IEImmunoelectrophoresis
PAPeptide Array
ICImmunocytochemistry
PEPeptide ELISA
MDMeDIP
SHIn situ hybridization
IAEnzyme Immunoassay
SEsandwich ELISA
PLProximity Ligation Assay
ECELISA(Cap)
EDELISA(Det)
BIBioimaging
IOImmunoassay
LFLateral Flow Immunoassay
LALuminex Assay
CImmunohistochemistry-Frozen Sections
PImmunohistologyp-Paraffin Sections
ISIntracellular Staining for Flow Cytometry
MSElectrophoretic Mobility Shift Assay
RIRNA Binding Protein Immunoprecipitation (RIP)
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