Mouse Anti-AKR1B1 Antibody (V2-2449) (CBMAB-1001CQ)

Name: Mouse Anti-AKR1B1 Antibody (V2-2449)
SKU: CBMAB-1001CQ
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AKR1B1 Antibody (H6) in IF and WB

Epalrestat effectively reduced aldose reductase (AR) expression and the apoptosis of endothelial cells in vivo after ischemia. (A) AR protein expression in different groups after permanent middle carotid artery ligation (pMCAL). (**P < 0.01; n = 5 per group). Eps = epalrestat. (B) AR (red) and CD31 (green) co-staining around the peri-infarction area in different groups. Scale bar = 10 μm. (**P < 0.01, ***P < 0.001; n = 5 per group). (C) Expression of apoptosis-related proteins in the ischemic brains. (*P < 0.05, ** P < 0.01, ***P < 0.001; n = 5 per group). (D) Cleaved-caspase3 (red) and CD31 (green) co-staining around the peri-infarction area in different groups. Scale bar = 10 μm. (**P < 0.01, ***P < 0.001; n = 5 per group). (E) Cerebral microvascular endothelial cell apoptosis in the ischemic brain tissue as detected by TUNEL (green)/CD31 (red) immunofluorescence double staining. Scale bar = 10 μm. (*P < 0.05; n = 5 per group). Data represent the mean ± SD ...View More

Zhang, T., Wu, J., Yao, X., Zhang, Y., Wang, Y., Han, Y., ... & Wang, G. (2023). The Aldose Reductase Inhibitor Epalrestat Maintains Blood-Brain Barrier Integrity by Enhancing Endothelial Cell Function during Cerebral Ischemia. Molecular Neurobiology, 60(7), 3741-3757.

Mouse Anti-AKR1B1 Antibody (H6) in WB

Western blot was used to detect the expression of AR in cultured podocytes, and the representative pictures. ...View More

He, J. Y., Hong, Q., Chen, B. X., Cui, S. Y., Liu, R., Cai, G. Y., ... & Chen, X. M. (2022). Ginsenoside Rb1 alleviates diabetic kidney podocyte injury by inhibiting aldose reductase activity. Acta Pharmacologica Sinica, 43(2), 342-353.

Mouse Anti-AKR1B1 Antibody (H6) in WB

Western blotting for detecting the effects of NBT (20 μM) on protein expression of RUNX2, ABCG2, and AKR1B1 for 3 days. ...View More

Xu, K., Huang, Y., Zhou, T., Wang, C., Chi, Q., Shi, J., ... & Dong, N. (2019). Nobiletin exhibits potent inhibition on tumor necrosis factor alpha-induced calcification of human aortic valve interstitial cells via targeting ABCG2 and AKR1B1. Phytotherapy Research, 33(6), 1717-1725.

Mouse Anti-AKR1B1 Antibody (H6) in WB

Immunoblots showing expression of aldose reductase and ERK1/2 in the adult rat adrenal gland and rat DRG tissue. ...View More

Schartner, E., Sabbir, M. G., Saleh, A., Silva, R. V., Chowdhury, S. R., Smith, D. R., & Fernyhough, P. (2018). High glucose concentration suppresses a SIRT2 regulated pathway that enhances neurite outgrowth in cultured adult sensory neurons. Experimental Neurology, 309, 134-147.

Mouse Anti-AKR1B1 Antibody (H6) in IF

Immunofluorescence staining for AR at 8 weeks for three groups including the control group (A, equatorial zone; E, anterior central zone), DM group (B, equatorial zone; F, anterior central zone), and DM+NGF group (C, equatorial zone; G, anterior central zone). At the equatorial zone and anterior central zone of lens, AR expression, localized in the LEC cytoplasm, was significantly increased in the DM group compared with the control group; however, it was significantly decreased in the DM+NGF group (D, equatorial zone; H, anterior central zone). *P < 0.05 between control and DM groups or between DM and DM+NGF groups. ...View More

Kim, J. Y., Park, J. H., Kang, S. S., Hwang, S. B., & Tchah, H. (2021). Topical nerve growth factor attenuates streptozotocin-induced diabetic cataracts via polyol pathway inhibition and Na+/K+-ATPase upregulation. Experimental Eye Research, 202, 108319.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-2449

Application

IF, IP, WB, ELISA

Immunogen

Amino acids 118-137 within an internal region of Aldose Reductase of mouse origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Aldo-Keto Reductase Family 1 Member B

Introduction

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Diseases associated with AKR1B1 include Diabetic Neuropathy and Diabetic Cataract. Among its related pathways are acetone degradation I (to methylglyoxal) and Metabolism.

Entrez Gene ID

231

UniProt ID

P15121

Alternative Names

AR; ADR; ALR2; ALDR1

Function

Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia (PubMed:1936586). Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal (PubMed:12732097, PubMed:19010934, PubMed:8343525). Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides (PubMed:17381426). Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (PubMed:21329684).

Biological Process

C21-steroid hormone biosynthetic process Source: Reactome
Carbohydrate metabolic process Source: ProtInc
Cellular hyperosmotic salinity response Source: UniProtKB
Cellular response to methylglyoxal Source: Ensembl
Cellular response to peptide Source: Ensembl
Daunorubicin metabolic process Source: UniProtKB
Doxorubicin metabolic process Source: UniProtKB
Epithelial cell maturation Source: Ensembl
Fructose biosynthetic process Source: Reactome
Inner medullary collecting duct development Source: Ensembl
Maternal process involved in female pregnancy Source: Ensembl
Metanephric collecting duct development Source: Ensembl
Naphthalene metabolic process Source: Ensembl
Negative regulation of apoptotic process Source: Ensembl
Norepinephrine metabolic process Source: Ensembl
Positive regulation of receptor signaling pathway via JAK-STAT Source: Ensembl
Positive regulation of smooth muscle cell proliferation Source: Ensembl
Regulation of urine volume Source: Ensembl
Renal water homeostasis Source: Ensembl
Response to thyroid hormone Source: Ensembl
Response to water deprivation Source: Ensembl
Retinoid metabolic process Source: UniProtKB
Sorbitol biosynthetic process Source: Ensembl
Stress-activated protein kinase signaling cascade Source: Ensembl
Tissue homeostasis Source: Ensembl

Schwab, A., Siddiqui, A., Vazakidou, M. E., Napoli, F., Boettcher, M., Menchicchi, B., ... & Ceppi, P. (2017). Aldo-keto reductase family 1 member b1 links glucose metabolism to epithelial-to-mesenchymal transition.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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