THOC5

THOC5 (THO Complex 5) is a protein coding gene. Among its related pathways are RNA Polymerase II Transcription Termination and Gene Expression. Gene Ontology annotations related to this gene include mRNA binding.

Full Name

THO Complex 5

Function

Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in transcription elongation and genome stability. Involved in alternative polyadenylation site choice by recruiting CPSF6 to 5' region of target genes; probably mediates association of the TREX and CFIm complexes.
Regulates the expression of myeloid transcription factors CEBPA, CEBPB and GAB2 by enhancing the levels of phosphatidylinositol 3,4,5-trisphosphate. May be involved in the differentiation of granulocytes and adipocytes. Essential for hematopoietic primitive cell survival and plays an integral role in monocytic development.

Biological Process

Biological Process monocyte differentiationSource:UniProtKB1 Publication
Biological Process mRNA export from nucleusSource:UniProtKB1 Publication
Biological Process mRNA processingSource:UniProtKB-KW
Biological Process negative regulation of DNA damage checkpointSource:UniProtKB1 Publication
Biological Process positive regulation of DNA-templated transcription, elongationSource:UniProtKB1 Publication
Biological Process primitive hemopoiesisSource:UniProtKB
Biological Process RNA splicingSource:UniProtKB-KW
Biological Process viral mRNA export from host cell nucleusSource:UniProtKB1 Publication

Cellular Location

Nucleus
Cytoplasm
Shuttles between nucleus and cytoplasm.

PTM

Phosphorylated on tyrosine upon binding to activated CSF1R; which causes a dissociation of the two proteins. Phosphorylation on Ser-5 and/or Ser-6 is required for nuclear export. Phosphorylated on Thr-328 in insulin-stimulated adipocytes (By similarity).
Phosphorylation at Tyr-225 modulates mRNA binding.