Mouse Anti-BLNK Recombinant Antibody (CBYY-0623) (CBMAB-0626-YY)

Name: Mouse Anti-BLNK Recombinant Antibody (CBYY-0623)
SKU: CBMAB-0626-YY
Rating: 5 (1 reviews)

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Published Data

CBMAB-0626-YY in WB

BLNK and BTK gene KO confers resistance to RTX in OCI-Ly-7 GCB subclass cells. ...View More

Thomsen, E. A., Rovsing, A. B., Anderson, M. V., Due, H., Huang, J., Luo, Y., ... & Mikkelsen, J. G. (2020). Identification of BLNK and BTK as mediators of rituximab-induced programmed cell death by CRISPR screens in GCB-subtype diffuse large B-cell lymphoma. Molecular Oncology, 14(9), 1978-1997.

CBMAB-0626-YY in WB

Western blot analysis of SLP65 expression in HL double KO and HLSLP65 triple KO Ramos cells. ...View More

He, X., Kläsener, K., Iype, J. M., Becker, M., Maity, P. C., Cavallari, M., ... & Reth, M. (2018). Continuous signaling of CD 79b and CD 19 is required for the fitness of Burkitt lymphoma B cells. The EMBO journal, 37(11), e97980.

CBMAB-0626-YY in IHC

Immunostaining of pDC-associated markers in tumors derived from these cells and in cutaneous deposits of acute myeloid leukemia (leukemia cutis). The markers shown were expressed by essentially all cases of pDC-derived neoplasms, and the first 4 were expressed only in a minority of leukemia cutis biopsies (BCL11A: 6 of 24 cases; BLNK: 8 of 24 cases; CD2AP: 1 of 24 cases, and ICSBP: 5 of 24 cases). ...View More

Marafioti, T., Paterson, J. C., Ballabio, E., Reichard, K. K., Tedoldi, S., Hollowood, K., ... & Mason, D. Y. (2008). Novel markers of normal and neoplastic human plasmacytoid dendritic cells. Blood, The Journal of the American Society of Hematology, 111(7), 3778-3792.

CBMAB-0626-YY in WB

Western Blot analysis after siRNA-mediated MYC knock-down in 3 BL cell lines.
An up-regulation of selected NF-kB pathway proteins (A20, CIAP2) and B-cell genes (CD20, BLNK, PAX5, CD79a) was detected by Western blot analysis of protein extracts derived from control siRNA-treated and MYC siRNA-treated BL cell lines. β-Actin (one example shown) was used as loading control. ...View More

Seitz, V., Butzhammer, P., Hirsch, B., Hecht, J., Gütgemann, I., Ehlers, A., ... & Hummel, M. (2011). Deep sequencing of MYC DNA-binding sites in Burkitt lymphoma. PloS one, 6(11), e26837.

CBMAB-0626-YY in WB

Coprecipitation of effector molecules by CD22 cytoplasmic domain fusion proteins. Purified splenic B cells (2 × 107 cells/sample) incubated in media or with anti-IgM antibodies were lysed and incubated with GST-CD22cyt or GST-CD22cyt(Tyr(P)) fusion proteins prior to capture with glutathione-Sepharose beads. Coprecipitated proteins were subjected to SDS-PAGE and electrophoretic transfer to nitrocellulose membranes. Immunoblotting was carried out using antibodies reactive with the indicated effector proteins. Data are representative of at least three independent experiments that generated similar results. ...View More

Poe, J. C., Fujimoto, M., Jansen, P. J., Miller, A. S., & Tedder, T. F. (2000). CD22 forms a quaternary complex with SHIP, Grb2, and Shc: a pathway for regulation of B lymphocyte antigen receptor-induced calcium flux. Journal of Biological Chemistry, 275(23), 17420-17427.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYY-0623

Application

WB, IP, IF, E, P

Immunogen

Amino acids 4-205 mapping at the N-terminus of BLNK of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

0.09% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

B Cell Linker

Introduction

This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]

Entrez Gene ID

29760

UniProt ID

Q8WV28

Alternative Names

B Cell Linker; B-Cell Adapter Containing A SH2 Domain Protein; Src Homology [SH2] Domain-Containing Leukocyte Protein Of 65 KD; Src Homology 2 Domain-Containing Leukocyte Protein Of 65 KDa; B-Cell Adapter Containing A Src Homology 2 Domain Protein; B Cell Adaptor Containing SH2 Domain; Cytoplasmic Adapter Protein; B-Cell Activation; SLP-65;

Function

Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca2+ mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.

Biological Process

B cell differentiation Source: UniProtKB
B cell receptor signaling pathway Source: UniProtKB
Humoral immune response Source: ProtInc
Inflammatory response Source: ProtInc
Intracellular signal transduction Source: UniProtKB
Positive regulation of gene expression Source: Ensembl
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central

Cellular Location

Cytoplasm; Cell membrane. BCR activation results in the translocation to membrane fraction.

Involvement in disease

Agammaglobulinemia 4, autosomal recessive (AGM4): A primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B-cells due to an early block of B-cell development. Affected individuals develop severe infections in the first years of life.

PTM

Following BCR activation, phosphorylated on tyrosine residues by SYK and LYN. When phosphorylated, serves as a scaffold to assemble downstream targets of antigen activation, including PLCG1, VAV1, GRB2 and NCK1. Phosphorylation of Tyr-84, Tyr-178 and Tyr-189 facilitates PLCG1 binding. Phosphorylation of Tyr-96 facilitates BTK binding. Phosphorylation of Tyr-72 facilitates VAV1 and NCK1 binding. Phosphorylation is required for both Ca2+ and MAPK signaling pathways.

Zhang, P., Wang, Y., Qin, M., Li, D., Odhiambo, W. O., Yuan, M., ... & Ji, Y. (2021). Involvement of Blnk and Foxo1 in tumor suppression in BCR‑ABL1‑transformed pro‑B cells. Oncology reports, 45(2), 693-705.

Singh, M. V., Wong, T., & Dokun, A. O. (2021). BLNK/BTK: Novel Components in the NF-kappa B Pathways of Endothelial Cells in Diabetic Condition. Journal of the Endoc

Lee, J. H., Lee, J. H., Ahn, B. K., Paik, S. S., & Lee, K. H. (2020). Prognostic value of B-cell linker protein in colorectal cancer. Pathology-Research and Practice, 216(3), 152821.

Thomsen, E. A., Rovsing, A. B., Anderson, M. V., Due, H., Huang, J., Luo, Y., ... & Mikkelsen, J. G. (2020). Identification of BLNK and BTK as mediators of rituximab‐induced programmed cell death by CRISPR screens in GCB‐subtype diffuse large B‐cell lymphoma. Molecular oncology, 14(9), 1978-1997.

Xiao, B., Li, J., Zhou, M., Li, X., Huang, X., Hang, J., ... & Li, L. (2019). Structure and function of B-cell linker and its role in the development of B cell-related diseases. Nan Fang yi ke da xue xue bao= Journal of Southern Medical University, 39(2), 253-256.

Mo, Z. Q., Wang, J. L., Han, R., Han, Q., Li, Y. W., Sun, H. Y., ... & Dan, X. M. (2018). Identification and functional analysis of grouper (Epinephelus coioides) B-cell linker protein BLNK. Fish & shellfish immunology, 81, 399-407.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

BLNK Matched Antibody Pair (141) (CAT#: APMAB-141LY)

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BLNK Matched Antibody Pair (141) (CAT#: APMAB-141LY)

Isotype control

For research use only. Not intended for any clinical use.

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