Mouse Anti-NSUN6 Recombinant Antibody (D-5) (CBMAB-N3674-WJ)

Name: Mouse Anti-NSUN6 Recombinant Antibody (D-5)
SKU: CBMAB-N3674-WJ
Rating: 5 (1 reviews)

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Tested Data

Fig.1 Western Blot analysis (antibody incubation concentration: 2ng/μL)<br />Lane 1: Reducing antigen (0.1μg)<br />Lane 2: Reducing antigen (0.3μg)<br />Lane 3: Reducing antigen (0.6μg)

Fig.1 Western Blot analysis (antibody incubation concentration: 2ng/μL)
Lane 1: Reducing antigen (0.1μg)
Lane 2: Reducing antigen (0.3μg)
Lane 3: Reducing antigen (0.6μg)

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

D-5

Application

WB, IP, IF, ELISA

Specificity

Mouse, Rat, Human

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Antibody

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

NOP2/Sun RNA Methyltransferase Family Member 6

Introduction

May have S-adenosyl-L-methionine-dependent methyl-transferase activity.

Entrez Gene ID

Human	221078
Mouse	74455
Rat	307148

UniProt ID

Human	Q8TEA1
Mouse	Q7TS68
Rat	B1WC69

Alternative Names

NOP2/Sun RNA Methyltransferase Family Member 6; NOL1/NOP2/Sun And PUA Domain-Containing Protein 1; NOL1/NOP2/Sun Domain Family, Member 6; NOP2/Sun Domain Family, Member 6; ARL5B Antisense RNA 1; NOPD1; Nucleolar Protein (NOL1/NOP2/Sun) And PUA Domains 1; NOL1/NOP2/Sun Domain Family Member 6;

Function

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA) (PubMed:26160102, PubMed:27703015, PubMed:28531330).
In vitro also methylates tRNA(Thr)(AGT) (PubMed:27703015, PubMed:26160102).
Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates (PubMed:26160102, PubMed:27703015).

Biological Process

RNA methylationManual Assertion Based On ExperimentIBA:GO_Central
tRNA C5-cytosine methylationManual Assertion Based On ExperimentIDA:UniProtKB
tRNA methylationManual Assertion Based On ExperimentIDA:UniProtKB
tRNA modificationTAS:Reactome

Cellular Location

Cytoplasm

PerezGrovas-Saltijeral, A., Rajkumar, A. P., & Knight, H. M. (2023). Differential expression of m5C RNA methyltransferase genes NSUN6 and NSUN7 in Alzheimer’s disease and traumatic brain injury. Molecular Neurobiology, 60(4), 2223-2235.

Mattioli, F., Worpenberg, L., Li, C. T., Ibrahim, N., Naz, S., Sharif, S., ... & Reymond, A. (2023). Biallelic variants in NSUN6 cause an autosomal recessive neurodevelopmental disorder. Genetics in Medicine, 25(9), 100900.

Zimmermann, R. A., Fischer, T. R., Schwickert, M., Nidoieva, Z., Schirmeister, T., & Kersten, C. (2023). Chemical space virtual screening against hard-to-drug RNA methyltransferases DNMT2 and NSUN6. International Journal of Molecular Sciences, 24(7), 6109.

Hu, S., Yang, M., Xiao, K., Yang, Z., Cai, L., Xie, Y., ... & Wei, R. (2023). Loss of NSUN6 inhibits osteosarcoma progression by downregulating EEF1A2 expression and activation of Akt/mTOR signaling pathway via m 5 C methylation. Experimental and Therapeutic Medicine, 26(3), 1-14.

Wang, W., Huang, H., Jiang, H., Tian, C., Tang, Y., Gan, D., ... & Fang, L. (2022). A cross-tissue investigation of molecular targets and physiological functions of Nsun6 using knockout mice. International Journal of Molecular Sciences, 23(12), 6584.

Awah, C. U., Winter, J., Mazdoom, C. M., & Ogunwobi, O. O. (2021). NSUN6, an RNA methyltransferase of 5-mC controls glioblastoma response to temozolomide (TMZ) via NELFB and RPS6KB2 interaction. Cancer biology & therapy, 22(10-12), 587-597.

Liu, J., Huang, T., Zhang, Y., Zhao, T., Zhao, X., Chen, W., & Zhang, R. (2021). Sequence-and structure-selective mRNA m5C methylation by NSUN6 in animals. National science review, 8(6), nwaa273.

Selmi, T., Hussain, S., Dietmann, S., Heiß, M., Borland, K., Flad, S., ... & Frye, M. (2021). Sequence-and structure-specific cytosine-5 mRNA methylation by NSUN6. Nucleic acids research, 49(2), 1006-1022.

Yang, R., Liang, X., Wang, H., Guo, M., Shen, H., Shi, Y., ... & Zhan, M. (2021). The RNA methyltransferase NSUN6 suppresses pancreatic cancer development by regulating cell proliferation. EBioMedicine, 63.

Li, J., Li, H., Long, T., Dong, H., Wang, E. D., & Liu, R. J. (2019). Archaeal NSUN6 catalyzes m5C72 modification on a wide-range of specific tRNAs. Nucleic acids research, 47(4), 2041-2055.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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