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Mouse Anti-NSUN6 Recombinant Antibody (D-5) (CBMAB-N3674-WJ)

This product is a Mouse antibody that recognizes NSUN6. The antibody D-5 can be used for immunoassay techniques such as: WB, IP, IF, ELISA.
See all NSUN6 antibodies
Published Data
Fig.1 Western Blot analysis (antibody incubation concentration: 2ng/μL)
Lane 1: Reducing antigen (0.1μg)
Lane 2: Reducing antigen (0.3μg)
Lane 3: Reducing antigen (0.6μg)
...View More

Summary

Host Animal
Mouse
Specificity
Mouse, Rat, Human
Clone
D-5
Application
WB, IP, IF, ELISA

Basic Information

Specificity
Mouse, Rat, Human
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Antibody
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
NOP2/Sun RNA Methyltransferase Family Member 6
Introduction
May have S-adenosyl-L-methionine-dependent methyl-transferase activity.
Entrez Gene ID
Human221078
Mouse74455
Rat307148
UniProt ID
HumanQ8TEA1
MouseQ7TS68
RatB1WC69
Alternative Names
NOP2/Sun RNA Methyltransferase Family Member 6; NOL1/NOP2/Sun And PUA Domain-Containing Protein 1; NOL1/NOP2/Sun Domain Family, Member 6; NOP2/Sun Domain Family, Member 6; ARL5B Antisense RNA 1; NOPD1; Nucleolar Protein (NOL1/NOP2/Sun) And PUA Domains 1; NOL1/NOP2/Sun Domain Family Member 6;
Function
S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA) (PubMed:26160102, PubMed:27703015, PubMed:28531330).
In vitro also methylates tRNA(Thr)(AGT) (PubMed:27703015, PubMed:26160102).
Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates (PubMed:26160102, PubMed:27703015).
Biological Process
RNA methylationManual Assertion Based On ExperimentIBA:GO_Central
tRNA C5-cytosine methylationManual Assertion Based On ExperimentIDA:UniProtKB
tRNA methylationManual Assertion Based On ExperimentIDA:UniProtKB
tRNA modificationTAS:Reactome
Cellular Location
Cytoplasm

PerezGrovas-Saltijeral, A., Rajkumar, A. P., & Knight, H. M. (2023). Differential expression of m5C RNA methyltransferase genes NSUN6 and NSUN7 in Alzheimer’s disease and traumatic brain injury. Molecular Neurobiology, 60(4), 2223-2235.

Mattioli, F., Worpenberg, L., Li, C. T., Ibrahim, N., Naz, S., Sharif, S., ... & Reymond, A. (2023). Biallelic variants in NSUN6 cause an autosomal recessive neurodevelopmental disorder. Genetics in Medicine, 25(9), 100900.

Zimmermann, R. A., Fischer, T. R., Schwickert, M., Nidoieva, Z., Schirmeister, T., & Kersten, C. (2023). Chemical space virtual screening against hard-to-drug RNA methyltransferases DNMT2 and NSUN6. International Journal of Molecular Sciences, 24(7), 6109.

Hu, S., Yang, M., Xiao, K., Yang, Z., Cai, L., Xie, Y., ... & Wei, R. (2023). Loss of NSUN6 inhibits osteosarcoma progression by downregulating EEF1A2 expression and activation of Akt/mTOR signaling pathway via m 5 C methylation. Experimental and Therapeutic Medicine, 26(3), 1-14.

Wang, W., Huang, H., Jiang, H., Tian, C., Tang, Y., Gan, D., ... & Fang, L. (2022). A cross-tissue investigation of molecular targets and physiological functions of Nsun6 using knockout mice. International Journal of Molecular Sciences, 23(12), 6584.

Awah, C. U., Winter, J., Mazdoom, C. M., & Ogunwobi, O. O. (2021). NSUN6, an RNA methyltransferase of 5-mC controls glioblastoma response to temozolomide (TMZ) via NELFB and RPS6KB2 interaction. Cancer biology & therapy, 22(10-12), 587-597.

Liu, J., Huang, T., Zhang, Y., Zhao, T., Zhao, X., Chen, W., & Zhang, R. (2021). Sequence-and structure-selective mRNA m5C methylation by NSUN6 in animals. National science review, 8(6), nwaa273.

Selmi, T., Hussain, S., Dietmann, S., Heiß, M., Borland, K., Flad, S., ... & Frye, M. (2021). Sequence-and structure-specific cytosine-5 mRNA methylation by NSUN6. Nucleic acids research, 49(2), 1006-1022.

Yang, R., Liang, X., Wang, H., Guo, M., Shen, H., Shi, Y., ... & Zhan, M. (2021). The RNA methyltransferase NSUN6 suppresses pancreatic cancer development by regulating cell proliferation. EBioMedicine, 63.

Li, J., Li, H., Long, T., Dong, H., Wang, E. D., & Liu, R. J. (2019). Archaeal NSUN6 catalyzes m5C72 modification on a wide-range of specific tRNAs. Nucleic acids research, 47(4), 2041-2055.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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