NSUN6
May have S-adenosyl-L-methionine-dependent methyl-transferase activity.
Full Name
NOP2/Sun RNA Methyltransferase Family Member 6
Function
S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA) (PubMed:26160102, PubMed:27703015, PubMed:28531330).
In vitro also methylates tRNA(Thr)(AGT) (PubMed:27703015, PubMed:26160102).
Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates (PubMed:26160102, PubMed:27703015).
In vitro also methylates tRNA(Thr)(AGT) (PubMed:27703015, PubMed:26160102).
Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates (PubMed:26160102, PubMed:27703015).
Biological Process
RNA methylationManual Assertion Based On ExperimentIBA:GO_Central
tRNA C5-cytosine methylationManual Assertion Based On ExperimentIDA:UniProtKB
tRNA methylationManual Assertion Based On ExperimentIDA:UniProtKB
tRNA modificationTAS:Reactome
tRNA C5-cytosine methylationManual Assertion Based On ExperimentIDA:UniProtKB
tRNA methylationManual Assertion Based On ExperimentIDA:UniProtKB
tRNA modificationTAS:Reactome
Cellular Location
Cytoplasm
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Anti-NSUN6 antibodies
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Target: NSUN6
Host: Mouse
Antibody Isotype: IgM
Specificity: Mouse, Rat, Human
Clone: D-5
Application*: WB, IP, IF, E
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For Research Use Only. Not For Clinical Use.
(P): Predicted
* Abbreviations
- AActivation
- AGAgonist
- APApoptosis
- BBlocking
- BABioassay
- BIBioimaging
- CImmunohistochemistry-Frozen Sections
- CIChromatin Immunoprecipitation
- CTCytotoxicity
- CSCostimulation
- DDepletion
- DBDot Blot
- EELISA
- ECELISA(Cap)
- EDELISA(Det)
- ESELISpot
- EMElectron Microscopy
- FFlow Cytometry
- FNFunction Assay
- GSGel Supershift
- IInhibition
- IAEnzyme Immunoassay
- ICImmunocytochemistry
- IDImmunodiffusion
- IEImmunoelectrophoresis
- IFImmunofluorescence
- IGImmunochromatography
- IHImmunohistochemistry
- IMImmunomicroscopy
- IOImmunoassay
- IPImmunoprecipitation
- ISIntracellular Staining for Flow Cytometry
- LALuminex Assay
- LFLateral Flow Immunoassay
- MMicroarray
- MCMass Cytometry/CyTOF
- MDMeDIP
- MSElectrophoretic Mobility Shift Assay
- NNeutralization
- PImmunohistologyp-Paraffin Sections
- PAPeptide Array
- PEPeptide ELISA
- PLProximity Ligation Assay
- RRadioimmunoassay
- SStimulation
- SESandwich ELISA
- SHIn situ hybridization
- TCTissue Culture
- WBWestern Blot
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