Mouse Anti-AMACR Recombinant Antibody (CB34A) (CBMAB-CA034LY)

Name: Mouse Anti-AMACR Recombinant Antibody (CB34A)
SKU: CBMAB-CA034LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AMACR Recombinant Antibody (CB34A) in IHC

a, b The pathological diagnosis of first operation was hybrid (clear cell and papillary type) renal cell carcinoma (nuclear grading III). c, d The final pathological result of reoperation showed the necrotic and hematoma tissue blended with RCC tumor cells (nuclear grading IV). e-h The results of immunohistochemical staining from the specimens of 1st operation. (e Pax8, f CA9, g CK7, and h AMACAR). i-l The results of immunohistochemical staining from the specimens of 2nd operation. (i Pax8, j CA9, k CK7, and l AMACAR). ...View More

Zhang, X., Yan, L., Yuan, X., Bai, T., Zhang, L., & Han, S. (2020). Rapid exacerbation featuring acute leukemoid reaction after retrolaparoscopic nephrectomy: a rare case report of renal cell carcinoma with postoperative comprehensive genomic profiling. World Journal of Surgical Oncology, 18, 1-9.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CB34A

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 281-382 mapping at the C-terminus of P504S of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:30-1:3,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Alpha-Methylacyl-CoA Racemase

Introduction

This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011]

Entrez Gene ID

23600

UniProt ID

Q9UHK6

Alternative Names

Alpha-Methylacyl-CoA Racemase; 2-Methylacyl-CoA Racemase; EC 5.1.99.4; AMACRD; CBAS4; P504S; RACE; RM;

Function

Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters (PubMed:7649182, PubMed:10655068, PubMed:11060359). Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs (PubMed:7649182, PubMed:10655068, PubMed:11060359).

Biological Process

Bile acid biosynthetic process Source: Reactome
Bile acid metabolic process Source: UniProtKB
Fatty acid beta-oxidation using acyl-CoA oxidase Source: Reactome
Protein localization Source: Reactome

Cellular Location

Peroxisome; Mitochondrion

Involvement in disease

Alpha-methylacyl-CoA racemase deficiency (AMACRD): A rare autosomal recessive peroxisomal disorder characterized by elevated plasma concentrations of pristanic acid C27-bile-acid intermediates, and adult onset of variable neurodegenerative symptoms affecting the central and peripheral nervous systems. Features may include seizures, visual failure, sensorimotor neuropathy, spasticity, migraine, and white matter hyperintensities on brain imaging.
Congenital bile acid synthesis defect 4 (CBAS4): A disorder characterized by the presence of trihydroxycoprostanic acid in the bile and absence of cholic acid. Patients manifest neonatal jaundice, intrahepatic cholestasis and bile duct deficiency.

Alsalamah, A. K., & Khan, A. O. (2021). Asymptomatic retinal dysfunction in alpha-methylacyl-CoA racemase deficiency. Molecular Vision, 27, 396.

Lee, H., Kim, M., Kim, S. H., Tran, Q., Kong, G., Kim, C., ... & Park, J. (2020). Alpha-Methylacyl-CoA racemase (AMACR), a potential new biomarker for glioblastoma. Frontiers in Oncology, 10.

Kumar, S., Bukhari, U., Sikandar, B., George, A., Memon, Y., Khan, N., & Bukhari, A. (2020). Sensitivity of Alpha-Methylacyl-COA Racemase (AMACR) Staining in Prostatic Adenocarcinoma. Journal of Liaquat University of Medical & Health Sciences, 19(04), 275-279.

Neal, D. J., Amin, M. B., & Smith, S. C. (2020). CK20 versus AMACR and p53 immunostains in evaluation of Urothelial Carcinoma in Situ and Reactive Atypia. Diagnostic Pathology, 15, 1-4.

Shapovalova, M., Davydova, J., Henzler, C., Daniel, M., Dehm, S. M., Warlick, C. A., & LeBeau, A. M. (2019). Correction: Exploiting the transcriptional specificity of the alpha-methylacyl-CoA racemase AMACR promoter for the molecular imaging of prostate cancer. Oncotarget, 10(47), 4920.

Kanwal, S., Perveen, S., & Arshad, H. M. (2018). Role of Alpha-methylacyl-CoA racemase gene in pathogenecity of CMT patients. J. Pak. Med. Assoc, 68, 1039-1042.

Shapovalova, M., Davydova, J., Henzler, C., Daniel, M., Dehm, S. M., Warlick, C. A., & LeBeau, A. M. (2018). Exploiting the transcriptional specificity of the alpha-methylacyl-CoA racemase AMACR promoter for the molecular imaging of prostate cancer. Oncotarget, 9(94), 36693.

Erdmann, K., Kaulke, K., Rieger, C., Wirth, M. P., & Fuessel, S. (2017). Induction of alpha-methylacyl-CoA racemase by miR-138 via up-regulation of β-catenin in prostate cancer cells. Journal of cancer research and clinical oncology, 143(11), 2201-2210.

Tariq, H., Ahmed, R., Afzal, S., Hashmi, S. N., & Hamdani, S. N. R. (2017). Immunohistochemical expression of alpha methylacyl-coa racemase (amacr) in carcinoma prostate in pakistani population. PAFMJ, 67(6), 1054-57.

Kovaleva, O. V., Samoilova, D. V., Shitova, M. S., Oleinikova, N. A., Danilova, N. V., Malkov, P. G., & Gratchev, A. (2017). A Novel Monoclonal Antibody Against Alpha-Methylacyl-CoA Racemase Applicable for Paraffin-Embedded Tissues and Diagnostics of Prostate Cancer. Monoclonal antibodies in immunodiagnosis and immunotherapy, 36(1), 30-34.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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