Anti-AMACR Antibody (CBMAB-A2485-YC) Immunohistochemistry Kit (V2LY-0826-LY203)

Name: Anti-AMACR Antibody (CBMAB-A2485-YC) Immunohistochemistry Kit
SKU: V2LY-0826-LY203
Rating: 5 (1 reviews)

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Datasheet

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Datasheet Components References Q & As Review & reward Protocols Associated Products

Summary

Product Overview

This comprehensive, all-in-one kit is designed for the precise immunohistochemical (IHC) detection of target protein in tissue samples. Unlike standard detection kits, it includes a highly specific mouse primary antibody bundled directly with our robust Polymer HRP detection system. The kit provides every essential reagent needed for the entire IHC workflow-from the initial antigen retrieval buffer to the final mounting media. By offering a pre-matched primary antibody with optimized buffers and secondary detection reagents, this kit eliminates the need for tedious protocol optimization and reagent sourcing, ensuring consistent, high-quality staining results.

Primary Antibody Cat.

CBMAB-A2485-YC

Primary Antibody

Mouse Anti-CD163 Recombinant Antibody (ED2)

Secondary Antibody Host Species

Goat

Secondary Antibody Species Specificity

Mouse

Application

IHC-P

Sample Type

FFPE tissue

Test Size

50 Tests

Storage

Stored at 2-8°C. The kit is stable for 6 months from the date of receipt.

Key Features

Target-Specific Primary Antibody

Includes a highly validated mouse primary antibody, ensuring reliable and specific target localization.

Complete End-to-End Solution

Contains all 11 essential components required for a full IHC workflow, including Tris-EDTA Antigen Retrieval Buffer, Blocking Buffer, Polymer HRP-Goat anti-Mouse Secondary Antibody, DAB Chromogens, Counterstain, and Mounting Media.

Streamlined Workflow

Pre-optimized reagents work seamlessly with the included primary antibody, saving researchers significant time on antibody titration and protocol adjustments.

Sample Compatibility

Highly recommended and optimized for Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections.

Kit Capacity

Provides sufficient reagents (50T) to perform 50 tests, depending on tissue section size.

More Infomation

Sub Cat	Reagents	Size
LV01	Antigen Retrieval Buffer (50× Tris-EDTA)	100 ml	Inquiry
LV02	Washing Buffer (30×)	120 ml	Inquiry
LV03	Blocking Buffer	5 ml	Inquiry
LV04	Quenching buffer (RTU)	5 ml	Inquiry
LV05	Primary Antibody Diluent (RTU)	100 ml	Inquiry
LV06M	HRP-Goat anti-Mouse Secondary Antibody	5 ml	Inquiry
LV07	Chromogen Component A	0.2 ml	Inquiry
LV08	Chromogen Component B	4 ml	Inquiry
LV09	Signal Enhancer	5 ml	Inquiry
LV10	Counter Staining Reagent	5 ml	Inquiry
LV11	Mounting Media	5 ml	Inquiry

Alsalamah, A. K., & Khan, A. O. (2021). Asymptomatic retinal dysfunction in alpha-methylacyl-CoA racemase deficiency. Molecular Vision, 27, 396.

Lee, H., Kim, M., Kim, S. H., Tran, Q., Kong, G., Kim, C., ... & Park, J. (2020). Alpha-Methylacyl-CoA racemase (AMACR), a potential new biomarker for glioblastoma. Frontiers in Oncology, 10.

Kumar, S., Bukhari, U., Sikandar, B., George, A., Memon, Y., Khan, N., & Bukhari, A. (2020). Sensitivity of Alpha-Methylacyl-COA Racemase (AMACR) Staining in Prostatic Adenocarcinoma. Journal of Liaquat University of Medical & Health Sciences, 19(04), 275-279.

Neal, D. J., Amin, M. B., & Smith, S. C. (2020). CK20 versus AMACR and p53 immunostains in evaluation of Urothelial Carcinoma in Situ and Reactive Atypia. Diagnostic Pathology, 15, 1-4.

Shapovalova, M., Davydova, J., Henzler, C., Daniel, M., Dehm, S. M., Warlick, C. A., & LeBeau, A. M. (2019). Correction: Exploiting the transcriptional specificity of the alpha-methylacyl-CoA racemase AMACR promoter for the molecular imaging of prostate cancer. Oncotarget, 10(47), 4920.

Kanwal, S., Perveen, S., & Arshad, H. M. (2018). Role of Alpha-methylacyl-CoA racemase gene in pathogenecity of CMT patients. J. Pak. Med. Assoc, 68, 1039-1042.

Shapovalova, M., Davydova, J., Henzler, C., Daniel, M., Dehm, S. M., Warlick, C. A., & LeBeau, A. M. (2018). Exploiting the transcriptional specificity of the alpha-methylacyl-CoA racemase AMACR promoter for the molecular imaging of prostate cancer. Oncotarget, 9(94), 36693.

Erdmann, K., Kaulke, K., Rieger, C., Wirth, M. P., & Fuessel, S. (2017). Induction of alpha-methylacyl-CoA racemase by miR-138 via up-regulation of β-catenin in prostate cancer cells. Journal of cancer research and clinical oncology, 143(11), 2201-2210.

Tariq, H., Ahmed, R., Afzal, S., Hashmi, S. N., & Hamdani, S. N. R. (2017). Immunohistochemical expression of alpha methylacyl-coa racemase (amacr) in carcinoma prostate in pakistani population. PAFMJ, 67(6), 1054-57.

Kovaleva, O. V., Samoilova, D. V., Shitova, M. S., Oleinikova, N. A., Danilova, N. V., Malkov, P. G., & Gratchev, A. (2017). A Novel Monoclonal Antibody Against Alpha-Methylacyl-CoA Racemase Applicable for Paraffin-Embedded Tissues and Diagnostics of Prostate Cancer. Monoclonal antibodies in immunodiagnosis and immunotherapy, 36(1), 30-34.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Primary Antibody

Mouse Anti-AMACR Recombinant Antibody (2A10F3) (CAT#: CBMAB-A2485-YC)

Alternative Versions

Human AMACR ELISA Kit (CAT#: V2LY-0626-LY3793)

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Rabbit Anti-AMACR Monoclonal Antibody (CBFYR-0002) (CAT#: CBMAB-R0004-FY)

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Mouse Anti-AMACR Recombinant Antibody (1D8) (CAT#: CBMAB-A2483-YC)

Human AMACR ELISA Kit (CAT#: V2LY-0626-LY3793)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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