Mouse Anti-AMACR Recombinant Antibody (2A10F3) (CBMAB-A2485-YC)

Name: Mouse Anti-AMACR Recombinant Antibody (2A10F3)
SKU: CBMAB-A2485-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-AMACR Recombinant Antibody (2A10F3) in IF

Following treatment with EMCL at the indicated doses and PDTC (30 µM) for 24 h, the subcellular localizations of p50 and p65 in 786-0 cells were examined by confocal microscopy analysis(magnification, ×630). ...View More

Zhu, J., Zhao, J., Yu, Z., Shrestha, S., Song, J., Liu, W., ... & Song, X. (2018). Epoxymicheliolide, a novelguaiane-type sesquiterpene lactone, inhibits NF‑κB/COX‑2 signaling pathways by targeting leucine 281 and leucine 25 in IKKβ in renal cell carcinoma. International Journal of Oncology, 53(3), 987-1000.

Mouse Anti-AMACR Recombinant Antibody (2A10F3) in WB

Following treatment with EMCL at the indicated doses and PDTC (30 µM) for 24 h, cytoplasmic and nuclear extracts were prepared for the western blot analysis of p-p65, p65, and p50. ...View More

Mouse Anti-AMACR Recombinant Antibody (2A10F3) in IHC

Pathological features with histological and immunohistochemical staining for P504S, PSA and CK7. Histological examination revealed a large island of tumor cells. Immunohistochemical staining revealed that the specimen cells were positive for P504S, PSA and CK7. PSA, prostate-specific antigen; CK7, cytokeratin 7; H&E, hematoxylin and eosin. ...View More

Sui, X., Hu, Y., Zhang, C., Pan, H., & Li, D. (2016). Prostate cancer metastasis to the distal phalanx of the left hallux: The first confirmed case and literature review. Oncology Letters, 12(2), 1074-1078.

Mouse Anti-AMACR Recombinant Antibody (2A10F3) in IHC

Exendin-4 and metformin decrease the number of Ki67 and P504S-positive cells, and increase GLP-1R expression.
Paraffin-embedded tumor sections (5 μm) were subjected to immunohistochemistry for (A) Ki67, (C) P504S and GLP-1R, and (F) AR, and counterstained with DAPI. Magnification, ×400. (B) Ki67, (D) P504S, (E) GLP-1R, and (G) AR-positive cells were quantified by analyzing the fraction of stained cells in the tumor relative to the total number of nuclei. Values are expressed as a percentage of positive cells. Unpaired t-tests were performed to calculate statistical significance (*P < 0.05, **P < 0.01 vs. control). ...View More

Tsutsumi, Y., Nomiyama, T., Kawanami, T., Hamaguchi, Y., Terawaki, Y., Tanaka, T., ... & Yanase, T. (2015). Combined treatment with exendin-4 and metformin attenuates prostate cancer growth. PLoS One, 10(10), e0139709.

Mouse Anti-AMACR Recombinant Antibody (2A10F3) in IHC

Immunohistochemical staining of a needle biopsy containing normal prostate tissue with the two-marker cocktail. Benign basal epithelial cells show dark brown nuclear staining for p63. There is no positive staining for AMACR (x 200). ...View More

Dabir, P. D., Ottosen, P., Høyer, S., & Hamilton-Dutoit, S. (2012). Comparative analysis of three-and two-antibody cocktails to AMACR and basal cell markers for the immunohistochemical diagnosis of prostate carcinoma. Diagnostic pathology, 7(1), 1-6.

Mouse Anti-AMACR Recombinant Antibody (2A10F3) in FC

The expression level of the prostate markers P504S (AMACR) and PSMA was evaluated by flow cytometry. LNCaP cells were used as controls. ...View More

Chauchereau, A., Al Nakouzi, N., Gaudin, C., Le Moulec, S., Compagno, D., Auger, N., ... & Fizazi, K. (2011). Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer. Experimental cell research, 317(3), 262-275.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

2A10F3

Application

WB, IHC-P, ICC, IF, IP

Immunogen

Amino acids 132-321 of P504S of human origin.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Alpha-Methylacyl-CoA Racemase

Introduction

AMACR is a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. En

Entrez Gene ID

23600

UniProt ID

Q9UHK6

Alternative Names

Macr1

Function

Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters (PubMed:7649182, PubMed:10655068, PubMed:11060359). Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs (PubMed:7649182, PubMed:10655068, PubMed:11060359).

Biological Process

Bile acid biosynthetic process Source: Reactome
Bile acid metabolic process Source: UniProtKB
Fatty acid beta-oxidation using acyl-CoA oxidase Source: Reactome
Protein localization Source: Reactome

Cellular Location

Peroxisome; Mitochondrion

Involvement in disease

Alpha-methylacyl-CoA racemase deficiency (AMACRD): A rare autosomal recessive peroxisomal disorder characterized by elevated plasma concentrations of pristanic acid C27-bile-acid intermediates, and adult onset of variable neurodegenerative symptoms affecting the central and peripheral nervous systems. Features may include seizures, visual failure, sensorimotor neuropathy, spasticity, migraine, and white matter hyperintensities on brain imaging.
Congenital bile acid synthesis defect 4 (CBAS4): A disorder characterized by the presence of trihydroxycoprostanic acid in the bile and absence of cholic acid. Patients manifest neonatal jaundice, intrahepatic cholestasis and bile duct deficiency.

Alsalamah, A. K., & Khan, A. O. (2021). Asymptomatic retinal dysfunction in alpha-methylacyl-CoA racemase deficiency. Molecular Vision, 27, 396.

Lee, H., Kim, M., Kim, S. H., Tran, Q., Kong, G., Kim, C., ... & Park, J. (2020). Alpha-Methylacyl-CoA racemase (AMACR), a potential new biomarker for glioblastoma. Frontiers in Oncology, 10.

Kumar, S., Bukhari, U., Sikandar, B., George, A., Memon, Y., Khan, N., & Bukhari, A. (2020). Sensitivity of Alpha-Methylacyl-COA Racemase (AMACR) Staining in Prostatic Adenocarcinoma. Journal of Liaquat University of Medical & Health Sciences, 19(04), 275-279.

Neal, D. J., Amin, M. B., & Smith, S. C. (2020). CK20 versus AMACR and p53 immunostains in evaluation of Urothelial Carcinoma in Situ and Reactive Atypia. Diagnostic Pathology, 15, 1-4.

Shapovalova, M., Davydova, J., Henzler, C., Daniel, M., Dehm, S. M., Warlick, C. A., & LeBeau, A. M. (2019). Correction: Exploiting the transcriptional specificity of the alpha-methylacyl-CoA racemase AMACR promoter for the molecular imaging of prostate cancer. Oncotarget, 10(47), 4920.

Kanwal, S., Perveen, S., & Arshad, H. M. (2018). Role of Alpha-methylacyl-CoA racemase gene in pathogenecity of CMT patients. J. Pak. Med. Assoc, 68, 1039-1042.

Shapovalova, M., Davydova, J., Henzler, C., Daniel, M., Dehm, S. M., Warlick, C. A., & LeBeau, A. M. (2018). Exploiting the transcriptional specificity of the alpha-methylacyl-CoA racemase AMACR promoter for the molecular imaging of prostate cancer. Oncotarget, 9(94), 36693.

Erdmann, K., Kaulke, K., Rieger, C., Wirth, M. P., & Fuessel, S. (2017). Induction of alpha-methylacyl-CoA racemase by miR-138 via up-regulation of β-catenin in prostate cancer cells. Journal of cancer research and clinical oncology, 143(11), 2201-2210.

Tariq, H., Ahmed, R., Afzal, S., Hashmi, S. N., & Hamdani, S. N. R. (2017). Immunohistochemical expression of alpha methylacyl-coa racemase (amacr) in carcinoma prostate in pakistani population. PAFMJ, 67(6), 1054-57.

Kovaleva, O. V., Samoilova, D. V., Shitova, M. S., Oleinikova, N. A., Danilova, N. V., Malkov, P. G., & Gratchev, A. (2017). A Novel Monoclonal Antibody Against Alpha-Methylacyl-CoA Racemase Applicable for Paraffin-Embedded Tissues and Diagnostics of Prostate Cancer. Monoclonal antibodies in immunodiagnosis and immunotherapy, 36(1), 30-34.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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