Human AMACR ELISA Kit (V2LY-0626-LY3793)

Name: Human AMACR ELISA Kit
SKU: V2LY-0626-LY3793
Rating: 5 (1 reviews)

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Tested Data

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Sensitivity

0.01 ng/mL

Detection Range

0.02-4.5 ng/mL

Sample Type

Serum, Plasma, cell culture supernates

Specificity

Human

Assay Type

Sandwich

Reactivity

Human

Assay Time

1.5 h

Molecule Mass

42.4 kDa

Components

Pre-coated ELISA Plate: 12 wells * 8 detachable strips
Standard solution: 0.5ml x1
Standard diluent: 3ml x1
Streptavidin-HRP: 6ml x1
Stop solution: 6ml x1
Substrate solution A: 6ml x1
Substrate solution B: 6ml x1
Wash buffer concentrate (25x): 20ml x1
Biotinylated antibody: 1ml x1

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at 2-8°C

More Infomation

Target

Full Name

Alpha-Methylacyl-CoA Racemase

Alternative Names

Alpha-Methylacyl-CoA Racemase; 2-Methylacyl-CoA Racemase; EC 5.1.99.4; AMACRD; CBAS4; P504S; RACE; RM;

Function

Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters (PubMed:7649182, PubMed:10655068, PubMed:11060359). Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs (PubMed:7649182, PubMed:10655068, PubMed:11060359).

Biological Process

Bile acid biosynthetic process Source: Reactome
Bile acid metabolic process Source: UniProtKB
Fatty acid beta-oxidation using acyl-CoA oxidase Source: Reactome
Protein localization Source: Reactome

Cellular Location

Peroxisome; Mitochondrion

Involvement in disease

Alpha-methylacyl-CoA racemase deficiency (AMACRD): A rare autosomal recessive peroxisomal disorder characterized by elevated plasma concentrations of pristanic acid C27-bile-acid intermediates, and adult onset of variable neurodegenerative symptoms affecting the central and peripheral nervous systems. Features may include seizures, visual failure, sensorimotor neuropathy, spasticity, migraine, and white matter hyperintensities on brain imaging.
Congenital bile acid synthesis defect 4 (CBAS4): A disorder characterized by the presence of trihydroxycoprostanic acid in the bile and absence of cholic acid. Patients manifest neonatal jaundice, intrahepatic cholestasis and bile duct deficiency.

Alsalamah, A. K., & Khan, A. O. (2021). Asymptomatic retinal dysfunction in alpha-methylacyl-CoA racemase deficiency. Molecular Vision, 27, 396.

Lee, H., Kim, M., Kim, S. H., Tran, Q., Kong, G., Kim, C., ... & Park, J. (2020). Alpha-Methylacyl-CoA racemase (AMACR), a potential new biomarker for glioblastoma. Frontiers in Oncology, 10.

Kumar, S., Bukhari, U., Sikandar, B., George, A., Memon, Y., Khan, N., & Bukhari, A. (2020). Sensitivity of Alpha-Methylacyl-COA Racemase (AMACR) Staining in Prostatic Adenocarcinoma. Journal of Liaquat University of Medical & Health Sciences, 19(04), 275-279.

Neal, D. J., Amin, M. B., & Smith, S. C. (2020). CK20 versus AMACR and p53 immunostains in evaluation of Urothelial Carcinoma in Situ and Reactive Atypia. Diagnostic Pathology, 15, 1-4.

Shapovalova, M., Davydova, J., Henzler, C., Daniel, M., Dehm, S. M., Warlick, C. A., & LeBeau, A. M. (2019). Correction: Exploiting the transcriptional specificity of the alpha-methylacyl-CoA racemase AMACR promoter for the molecular imaging of prostate cancer. Oncotarget, 10(47), 4920.

Kanwal, S., Perveen, S., & Arshad, H. M. (2018). Role of Alpha-methylacyl-CoA racemase gene in pathogenecity of CMT patients. J. Pak. Med. Assoc, 68, 1039-1042.

Shapovalova, M., Davydova, J., Henzler, C., Daniel, M., Dehm, S. M., Warlick, C. A., & LeBeau, A. M. (2018). Exploiting the transcriptional specificity of the alpha-methylacyl-CoA racemase AMACR promoter for the molecular imaging of prostate cancer. Oncotarget, 9(94), 36693.

Erdmann, K., Kaulke, K., Rieger, C., Wirth, M. P., & Fuessel, S. (2017). Induction of alpha-methylacyl-CoA racemase by miR-138 via up-regulation of β-catenin in prostate cancer cells. Journal of cancer research and clinical oncology, 143(11), 2201-2210.

Tariq, H., Ahmed, R., Afzal, S., Hashmi, S. N., & Hamdani, S. N. R. (2017). Immunohistochemical expression of alpha methylacyl-coa racemase (amacr) in carcinoma prostate in pakistani population. PAFMJ, 67(6), 1054-57.

Kovaleva, O. V., Samoilova, D. V., Shitova, M. S., Oleinikova, N. A., Danilova, N. V., Malkov, P. G., & Gratchev, A. (2017). A Novel Monoclonal Antibody Against Alpha-Methylacyl-CoA Racemase Applicable for Paraffin-Embedded Tissues and Diagnostics of Prostate Cancer. Monoclonal antibodies in immunodiagnosis and immunotherapy, 36(1), 30-34.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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