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Mouse Anti-AMACR Recombinant Antibody (2H9B5) (CBMAB-A2489-YC)


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Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
2H9B5
Antibody Isotype
IgG1
Application
FC, WB

Basic Information

Immunogen
p504S,AMACR Fusion Protein Ag8720 (1-135 aa).
Host Species
Mouse
Specificity
Human, Mouse
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.3, 50% glycerol
Preservative
0.02% sodium azide
Concentration
0.5 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Alpha-Methylacyl-CoA Racemase
Introduction
AMACR is a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. En
Entrez Gene ID
Human23600
Mouse17117
UniProt ID
HumanQ9UHK6
MouseO09174
Alternative Names
Alpha-Methylacyl-CoA Racemase; 2-Methylacyl-CoA Racemase; EC 5.1.99.4; AMACRD; CBAS4; P504S; RACE; RM;
Function
Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters (PubMed:7649182, PubMed:10655068, PubMed:11060359). Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs (PubMed:7649182, PubMed:10655068, PubMed:11060359).
Biological Process
Bile acid biosynthetic process Source: Reactome
Bile acid metabolic process Source: UniProtKB
Fatty acid beta-oxidation using acyl-CoA oxidase Source: Reactome
Protein localization Source: Reactome
Cellular Location
Peroxisome; Mitochondrion
Involvement in disease
Alpha-methylacyl-CoA racemase deficiency (AMACRD): A rare autosomal recessive peroxisomal disorder characterized by elevated plasma concentrations of pristanic acid C27-bile-acid intermediates, and adult onset of variable neurodegenerative symptoms affecting the central and peripheral nervous systems. Features may include seizures, visual failure, sensorimotor neuropathy, spasticity, migraine, and white matter hyperintensities on brain imaging.
Congenital bile acid synthesis defect 4 (CBAS4): A disorder characterized by the presence of trihydroxycoprostanic acid in the bile and absence of cholic acid. Patients manifest neonatal jaundice, intrahepatic cholestasis and bile duct deficiency.
More Infomation

Alsalamah, A. K., & Khan, A. O. (2021). Asymptomatic retinal dysfunction in alpha-methylacyl-CoA racemase deficiency. Molecular Vision, 27, 396.

Lee, H., Kim, M., Kim, S. H., Tran, Q., Kong, G., Kim, C., ... & Park, J. (2020). Alpha-Methylacyl-CoA racemase (AMACR), a potential new biomarker for glioblastoma. Frontiers in Oncology, 10.

Kumar, S., Bukhari, U., Sikandar, B., George, A., Memon, Y., Khan, N., & Bukhari, A. (2020). Sensitivity of Alpha-Methylacyl-COA Racemase (AMACR) Staining in Prostatic Adenocarcinoma. Journal of Liaquat University of Medical & Health Sciences, 19(04), 275-279.

Neal, D. J., Amin, M. B., & Smith, S. C. (2020). CK20 versus AMACR and p53 immunostains in evaluation of Urothelial Carcinoma in Situ and Reactive Atypia. Diagnostic Pathology, 15, 1-4.

Shapovalova, M., Davydova, J., Henzler, C., Daniel, M., Dehm, S. M., Warlick, C. A., & LeBeau, A. M. (2019). Correction: Exploiting the transcriptional specificity of the alpha-methylacyl-CoA racemase AMACR promoter for the molecular imaging of prostate cancer. Oncotarget, 10(47), 4920.

Kanwal, S., Perveen, S., & Arshad, H. M. (2018). Role of Alpha-methylacyl-CoA racemase gene in pathogenecity of CMT patients. J. Pak. Med. Assoc, 68, 1039-1042.

Shapovalova, M., Davydova, J., Henzler, C., Daniel, M., Dehm, S. M., Warlick, C. A., & LeBeau, A. M. (2018). Exploiting the transcriptional specificity of the alpha-methylacyl-CoA racemase AMACR promoter for the molecular imaging of prostate cancer. Oncotarget, 9(94), 36693.

Erdmann, K., Kaulke, K., Rieger, C., Wirth, M. P., & Fuessel, S. (2017). Induction of alpha-methylacyl-CoA racemase by miR-138 via up-regulation of β-catenin in prostate cancer cells. Journal of cancer research and clinical oncology, 143(11), 2201-2210.

Tariq, H., Ahmed, R., Afzal, S., Hashmi, S. N., & Hamdani, S. N. R. (2017). Immunohistochemical expression of alpha methylacyl-coa racemase (amacr) in carcinoma prostate in pakistani population. PAFMJ, 67(6), 1054-57.

Kovaleva, O. V., Samoilova, D. V., Shitova, M. S., Oleinikova, N. A., Danilova, N. V., Malkov, P. G., & Gratchev, A. (2017). A Novel Monoclonal Antibody Against Alpha-Methylacyl-CoA Racemase Applicable for Paraffin-Embedded Tissues and Diagnostics of Prostate Cancer. Monoclonal antibodies in immunodiagnosis and immunotherapy, 36(1), 30-34.

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For research use only. Not intended for any clinical use.

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