Mouse Anti-CORO1A Recombinant Antibody (4G10) (V2LY-1206-LY806)

Name: Mouse Anti-CORO1A Recombinant Antibody (4G10)
SKU: V2LY-1206-LY806
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-CORO1A Recombinant Antibody (4G10) (V2LY-1206-LY806) in IF

Sections from wild-type mouse brain were analyzed for the expression of coronin 1 (Alexa Fluor 488) and neurofilament-M chain (Alexa Fluor 546 and 568) as well as DRAQ5. Scale bar, 100 µm (Merge); 25 µm. ...View More

Jayachandran, R., Liu, X., BoseDasgupta, S., Müller, P., Zhang, C. L., Moshous, D., ... & Pieters, J. (2014). Coronin 1 regulates cognition and behavior through modulation of cAMP/protein kinase A signaling. PLoS biology, 12(3), e1001820.

Distributed under Open Access license CC BY 4.0, without modification.

Mouse Anti-CORO1A Recombinant Antibody (4G10) (V2LY-1206-LY806) in WB

Coronin 1-expressing Mel JuSo cells were left untreated or stimulated with isoproterenol (10 µM, 10 min), lysed, and Gα molecules were immunoprecipitated with the indicated antibodies as described, followed by separation on SDS-PAGE and immunoblotting using anti-coronin 1. ...View More

Distributed under Open Access license CC BY 4.0, without modification.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4G10

Application

ELISA, WB, IHC-P

Immunogen

CORO1A (aa360-461) partial recombinant protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC-P	3 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Coronin 1A

Entrez Gene ID

11151

UniProt ID

P31146

Function

May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes.

Biological Process

Actin cytoskeleton organization Source: UniProtKB
Actin filament organization Source: GO_Central
Calcium ion transport Source: Ensembl
Cell migration Source: GO_Central
Cell-substrate adhesion Source: UniProtKB
Cellular response to interleukin-4 Source: Ensembl
Early endosome to recycling endosome transport Source: Ensembl
Homeostasis of number of cells within a tissue Source: Ensembl
Innate immune response Source: UniProtKB
Leukocyte chemotaxis Source: Ensembl
Natural killer cell degranulation Source: UniProtKB
Negative regulation of actin nucleation Source: UniProtKB
Negative regulation of neuron apoptotic process Source: Ensembl
Negative regulation of vesicle fusion Source: Ensembl
Nerve growth factor signaling pathway Source: Ensembl
Phagocytosis Source: UniProtKB
Phagolysosome assembly Source: UniProtKB
Positive chemotaxis Source: UniProtKB
Positive regulation of cell migration Source: Ensembl
Positive regulation of T cell proliferation Source: Ensembl
Regulation of actin cytoskeleton organization Source: UniProtKB
Regulation of cell shape Source: Ensembl
Regulation of release of sequestered calcium ion into cytosol Source: Ensembl
T cell homeostasis Source: Ensembl
Uropod organization Source: Ensembl

Cellular Location

Cytoskeleton; Cell cortex; Phagosome membrane. In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification (By similarity).

Involvement in disease

Immunodeficiency 8 (IMD8):
A disease of the immune system leading to recurrent infections, and characterized by CD4+ T-cells lymphopenia. Patients can develop B-cell lymphoproliferation associated with Epstein-Barr virus infection.

PTM

phosphorylation at Thr-412 by PKC strongly down-regulates the association with actin.
Polyubiquitinated by RNF128 with 'Lys-48'-linked chains, leading to proteasomal degradation.

Wang, Y., Li, T., Li, H., Liang, Y., Mai, W., Liu, C., ... & Zhang, Q. (2022). CORO1A regulates lipoprotein uptake in Leydig cells exposed to cadmium. Ecotoxicology and Environmental Safety, 232, 113255.

Pandey, H. S., Kapoor, R., & Seth, P. (2020). Coronin 1A Expression in Human Astroglia, its Function in Physiology and Astrogliosis in HIV-1 Neuropathogenesis.

Nicolaou, O., Sokratous, K., Makowska, Z., Morell, M., De Groof, A., Montigny, P., ... & Kyriacou, K. (2020). Proteomic analysis in lupus mice identifies Coronin-1A as a potential biomarker for lupus nephritis. Arthritis research & therapy, 22(1), 1-16.

Riley, D. R., Khalil, J. S., Pieters, J., Naseem, K. M., & Rivero, F. (2020). Coronin 1 is required for integrin β2 translocation in platelets. International journal of molecular sciences, 21(1), 356.

Feng, J., Chen, S., Wang, Y., Liu, Q., Yang, M., Li, X., ... & Zhang, Q. (2019). Maternal exposure to cadmium impairs cognitive development of male offspring by targeting the Coronin-1a signaling pathway. Chemosphere, 225, 765-774.

Ford, M. L. (2019). Coronin-1, king of alloimmunity. Immunity, 50(1), 3-5.

Jayachandran, R., Gumienny, A., Bolinger, B., Ruehl, S., Lang, M. J., Fucile, G., ... & Pieters, J. (2019). Disruption of coronin 1 signaling in T cells promotes allograft tolerance while maintaining anti-pathogen immunity. Immunity, 50(1), 152-165.

Stocker, T. J., Pircher, J., Skenderi, A., Ehrlich, A., Eberle, C., Megens, R. T., ... & Schulz, C. (2018). The actin regulator coronin-1A modulates platelet shape change and consolidates arterial thrombosis. Thrombosis and haemostasis, 118(12), 2098-2111.

Pick, R., Begandt, D., Stocker, T. J., Salvermoser, M., Thome, S., Böttcher, R. T., ... & Walzog, B. (2017). Coronin 1A, a novel player in integrin biology, controls neutrophil trafficking in innate immunity. Blood, The Journal of the American Society of Hematology, 130(7), 847-858.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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