Human XL Oncology Antibody Array (Membrane, 84 Targets) (Y0236) (AbAr-0236-YC)

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Specifications

Target Array
XL Oncology Array
Target
AFP, ErbB4, MMP-2, Amphiregulin, FGF basic, MMP-3, ANGPT1, FoxC2 , MMP-9, ANGPTL4, FKHR, MSP/MST1, ENPP-2/Autotaxin, LGALS3, MUC-1, AXL, GM-CSF, Nectin-4, BCL-X, HCG, Osteopontin, CA125/MUC-16, HGF R/c-Met, p27/Kip1, E-Cadherin, HIF-1alpha, p53, VE-Cadherin, HNF-3beta, PDGF-AA, CAP-G, HMOX1, PECAM-1, CA-9, ICAM-1, Progesterone R, Cathepsin B, IL-2 R alpha, Progranulin, Cathepsin D, IL-6, Prolactin, CTSS, CXCL8, Prostasin, CEACAM-5, IL-18 Bpa, E-Selectin, Decorin, KLK-3/PSA, Maspin, DKK-1, KLK-5, PAI-1/Serpin E1, DLL-1, KLK-6, SNAIL, ErbB1, Leptin (OB), SPARC, Endoglin, Lumican, Survivin, Endostatin, CCL2, Tenascin-C, Enolase 2, CCL8, THBS-1, eNOS, CCL7, TIE-2, EpCAM, M-CSF, UPA-1, ER-alpha, Mesothelin, VCAM-1, ErbB2, CCL3, VEGF, ErbB3, CCL20/MIP-3alpha, Vimentin
Species Reactivity
Human

Applications

Application
Multiplex Protein Detection
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Apply a blocking agent to the membranes.
Proceed with sample incubation.
Incubate using the cocktail of biotinylated detection antibodies.
Incubate with streptavidin conjugated to horseradish peroxidase (HRP).
Conduct incubation with the detection buffers.
Utilize a chemiluminescent imaging system for image capture.
Execute densitometric evaluation and perform data analysis.
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.

Background

Introduction
A large number of genes with diverse normal functions are involved in human cancer. More than 500 genes have been identified as strongly implicated in the process of transforming normal cells to cancer cells. The expression of these genes in normal cells contributes to normal growth, survival and function, whereas dysregulated expression, including overexpression, loss of expression or expression of a defect protein, in cancer cells contributes to ungoverned tumor growth. Altered gene expression can be caused by coarse structural and numerical chromosomal rearrangements, specific gene amplifications, silencing of transcription through methylation and mutations, e.g. point mutations with single base substitutions and small inserts or deletions, that lead to loss or gain of function of the corresponding protein.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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