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Human XL Oncology Antibody Array (Membrane, 84 Targets) (Y0236) (AbAr-0236-YC)

Antibody array is a specific form of protein microarray. In this technology, capture antibodies spotted on membranes or glass slide bind to specific target proteins present in the sample. Captured proteins are visualized using chemiluminescent or Laser Scanner. The signal produced is proportional to the amount of analyte bound. The Human XL Oncology Antibody Array can be used to detect differences in 84 cancer-related proteins between samples.
Specifications
Target Array
XL Oncology Array
Target
AFP, ErbB4, MMP-2, Amphiregulin, FGF basic, MMP-3, Angiopoietin-1, FoxC2 , MMP-9, ANGPTL4, FKHR, MSP/MST1, ENPP-2/Autotaxin, Galectin-3, MUC-1, AXL, GM-CSF, Nectin-4, BCL-X, HCG, Osteopontin, CA125/MUC-16, HGF R/c-Met, p27/Kip1, E-Cadherin, HIF-1alpha, p53, VE-Cadherin, HNF-3beta, PDGF-AA, CAP-G, HO-1/HMOX1, CD31/PECAM-1, CA-9, ICAM-1/CD54, Progesterone R, Cathepsin B, CD25/IL-2 R alpha, Progranulin, Cathepsin D, IL-6, Prolactin, Cathepsin S, CXCL8/IL-8, Prostasin, CEACAM-5, IL-18 Bpa, E-Selectin, Decorin, KLK-3/PSA, Maspin, DKK-1, KLK-5, PAI-1/Serpin E1, DLL-1, KLK-6, SNAIL, EGF R/ErbB1, Leptin (OB), SPARC, Endoglin/CD105, Lumican, Survivin, Endostatin, CCL2/MCP-1, Tenascin-C, Enolase 2, CCL8/MCP-2, THBS-1, eNOS, CCL7/MCP-3, TIE-2, EpCAM, M-CSF, UPA-1, ER-alpha, Mesothelin, VCAM-1, ErbB2, CCL3/MIP-1alpha, VEGF, ErbB3, CCL20/MIP-3alpha, Vimentin
Species Reactivity
Human
Applications
Application
Multiplex Protein Detection
Solid Support
Membrane
Detection Assay
Sandwich-based
Protocol Outline
Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
Components
Antibody Array Membrane
Labeling Reagent
Blocking Buffer
Wash Buffer
Detection Buffer
Detection Cocktail
Others
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Once thawed, please keep reagents under suitable conditions respectively. Do not use past expiration date.
Background
Introduction
A large number of genes with diverse normal functions are involved in human cancer. More than 500 genes have been identified as strongly implicated in the process of transforming normal cells to cancer cells. The expression of these genes in normal cells contributes to normal growth, survival and function, whereas dysregulated expression, including overexpression, loss of expression or expression of a defect protein, in cancer cells contributes to ungoverned tumor growth. Altered gene expression can be caused by coarse structural and numerical chromosomal rearrangements, specific gene amplifications, silencing of transcription through methylation and mutations, e.g. point mutations with single base substitutions and small inserts or deletions, that lead to loss or gain of function of the corresponding protein.
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For Research Use Only. Not For Clinical Use.