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Rabbit Anti-AACS Recombinant Antibody (V2-178856) (CBMAB-A0073-YC)

Provided herein is a Rabbit monoclonal antibody against Human Acetoacetyl-CoA Synthetase. The antibody can be used for immunoassay techniques, such as ELISA.
See all AACS antibodies
Published Data
Rabbit Anti-AACS Recombinant Antibody (CBYC-A110) (CBMAB-A0073-YC) in WB
Sp1 binds in vitro and in vivo to the AACS promoter (A) DAPA of HIB-1B cells extracts incubated with biotinylated oligonucleotides containing the putative Sp1-binding sequence from the human AACS (Wt) or a scrambled sequence of these sites (Mut). WCE, whole cell extracts. Avidin-purified oligonucleotides were processed for Western blot analysis with Sp1-specific antibodies. ...View More
Aguiló, F., Camarero, N., Relat, J., Marrero, P. F., & Haro, D. (2010). Transcriptional regulation of the human acetoacetyl-CoA synthetase gene by PPARγ. Biochemical Journal, 427(2), 255-264.

Summary

Host Animal
Rabbit
Specificity
Human
Clone
V2-178856
Antibody Isotype
IgG
Application
ELISA

Basic Information

Immunogen
Recombinant Human Acetoacetyl-CoA Synthetase/AACS Protein
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:5,000-1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS
Preservative
None
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Acetoacetyl-CoA Synthetase
Introduction
Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development.
Entrez Gene ID
65985
UniProt ID
Q86V21
Alternative Names
Acetoacetyl-CoA Synthetase; Acyl-CoA Synthetase Family Member 1; Protein Sur-5 Homolog; EC 6.2.1.16; ACSF1; Homolog Of C. Elegans Supressor Of Ras 5 (Sur-5); Acetoacetate-CoA Ligase; EC 6.2.1; SUR-5;
Function
Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development (By similarity).
Biological Process
Adipose tissue development
Cellular response to cholesterol
Cellular response to glucose stimulus
Cellular response to testosterone stimulus
Fatty acid metabolic process
Ketone body biosynthetic process
Liver development
Positive regulation of insulin secretion
Response to drug
Response to ethanol
Response to nutrient
Response to oleic acid
Response to purine-containing compound
Response to starvation
White fat cell differentiation
Cellular Location
Cytosol
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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