What species reactivity does this Rabbit Anti-AACS Recombinant Antibody (CBMAB-A0073-YC) possess?

This recombinant antibody is specifically validated for reactivity with Human AACS. It has also shown excellent reactivity with Mouse AACS in our internal validation tests. While recombinant antibodies are generally known for their high specificity, cross-reactivity with AACS from other species is not guaranteed unless explicitly stated on the product datasheet. We recommend consulting the most current product information for detailed species reactivity data.

Is this antibody suitable for use in quantitative applications like ELISA?

While its primary validated applications include Western Blot, Immunofluorescence, and Immunoprecipitation, recombinant antibodies often perform very well in quantitative assays like ELISA due to their high specificity and consistent production. Users interested in ELISA applications are encouraged to perform initial optimization steps to determine the ideal antibody concentration and format (e.g., capture or detection antibody) for their specific ELISA protocol.

Rabbit Anti-AACS Recombinant Antibody (V2-178856) (CBMAB-A0073-YC)

See all AACS antibodies

Compare Online Inquiry

Published Data

Rabbit Anti-AACS Recombinant Antibody (CBYC-A110) (CBMAB-A0073-YC) in WB

Sp1 binds in vitro and in vivo to the AACS promoter (A) DAPA of HIB-1B cells extracts incubated with biotinylated oligonucleotides containing the putative Sp1-binding sequence from the human AACS (Wt) or a scrambled sequence of these sites (Mut). WCE, whole cell extracts. Avidin-purified oligonucleotides were processed for Western blot analysis with Sp1-specific antibodies. ...View More

Aguiló, F., Camarero, N., Relat, J., Marrero, P. F., & Haro, D. (2010). Transcriptional regulation of the human acetoacetyl-CoA synthetase gene by PPARγ. Biochemical Journal, 427(2), 255-264.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

V2-178856

Application

ELISA

Immunogen

Recombinant Human Acetoacetyl-CoA Synthetase/AACS Protein

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:5,000-1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Acetoacetyl-CoA Synthetase

Introduction

Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development.

Entrez Gene ID

65985

UniProt ID

Q86V21

Alternative Names

Acetoacetyl-CoA Synthetase; Acyl-CoA Synthetase Family Member 1; Protein Sur-5 Homolog; EC 6.2.1.16; ACSF1; Homolog Of C. Elegans Supressor Of Ras 5 (Sur-5); Acetoacetate-CoA Ligase; EC 6.2.1; SUR-5;

Function

Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development (By similarity).

Biological Process

Adipose tissue development
Cellular response to cholesterol
Cellular response to glucose stimulus
Cellular response to testosterone stimulus
Fatty acid metabolic process
Ketone body biosynthetic process
Liver development
Positive regulation of insulin secretion
Response to drug
Response to ethanol
Response to nutrient
Response to oleic acid
Response to purine-containing compound
Response to starvation
White fat cell differentiation

Cellular Location

Cytosol

Haydar, S., Grigorescu, F., Vintilă, M., Cogne, Y., Lautier, C., Tutuncu, Y., ... & Missoni, S. (2019). Fine-scale haplotype mapping of MUT, AACS, SLC6A15 and PRKCA genes indicates association with insulin resistance of metabolic syndrome and relationship with branched chain amino acid metabolism or regulation. PloS one, 14(3), e0214122.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-AACS Recombinant Antibody (V2-178856)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry