What species reactivity is guaranteed for this antibody?

The Recombinant Mouse Anti-AARS Recombinant Antibody (CBMAB-XB0006-YC) is specifically raised against human AARS. While its primary reactivity is validated for human samples, it may also cross-react with AARS from other species if there is sufficient sequence homology. We recommend checking the product datasheet for any officially tested and confirmed species reactivity. For unlisted species, preliminary testing is advised.

Is this a monoclonal or polyclonal antibody, and what are the advantages of its recombinant nature?

This product, CAT: CBMAB-XB0006-YC, is a recombinant mouse monoclonal antibody. The "recombinant" nature signifies that it is produced using molecular biology techniques, rather than traditional hybridoma methods. This offers several advantages, including superior batch-to-batch consistency, reduced variability, high purity, and often enhanced specificity due to precise genetic engineering. It typically ensures a more reliable and reproducible reagent for your experiments compared to traditional hybridoma-derived antibodies.

Recombinant Mouse Anti-AARS Recombinant Antibody (V2-444421) (CBMAB-XB0006-YC)

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Published Data

Recombinant Mouse Anti-AARS Recombinant Antibody (M6-P2E5) (CBMAB-XB0006-YC) in WB

The effect of knock-down of TRS or AlaRS with its specific siRNAs was determined by immunoblotting with each antibody ...View More

Jeong, S. J., Park, S., Nguyen, L. T., Hwang, J., Lee, E. Y., Giong, H. K., ... & Kim, S. (2019). A threonyl-tRNA synthetase-mediated translation initiation machinery. Nature communications, 10(1), 1357.

Recombinant Mouse Anti-AARS Recombinant Antibody (M6-P2E5) (CBMAB-XB0006-YC) in IF

Interactions between the indicated protein pairs determined by reconstitution of Venus green fluorescent protein (GFP) in Chinese hamster ovary (CHO) cells. Nuclei were stained with 4',6-diamidino-2 phenylindole (DAPI; blue). Expression of HA-4EHP and FLAG-TRS was confirmed by immunofluorescence staining with anti-HA (Alexa 647; purple) and anti-FLAG (Alexa 594; red) antibodies, respectively. Scale bar = 10 μM. ...View More

Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-444421

Application

ELISA, IHC, WB

Immunogen

Peptide-TSFAQLRLGD (amino acid 956-965)

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
ELISA	1:30-1:3,000
IF(ICC)	1:50-1:500
IP	1-2 µl per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Alanyl-TRNA Synthetase

Introduction

The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.

Entrez Gene ID

UniProt ID

P49588

Alternative Names

Alanyl-TRNA Synthetase; Alanine TRNA Ligase 1, Cytoplasmic; Renal Carcinoma Antigen NY-REN-42; EC 6.1.1.7; AlaRS; Alanyl-TRNA Synthetase, Cytoplasmic;

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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