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Recombinant Mouse Anti-AARS Recombinant Antibody (V2-444421) (CBMAB-XB0006-YC)

Provided herein is a Mouse Recombinant Antibody against Alanyl-TRNA Synthetase. The antibody can be used for immunoassay techniques, such as ELISA, IHC, WB.
See all AARS antibodies
Published Data
Recombinant Mouse Anti-AARS Recombinant Antibody (M6-P2E5) (CBMAB-XB0006-YC) in WB
The effect of knock-down of TRS or AlaRS with its specific siRNAs was determined by immunoblotting with each antibody ...View More
Jeong, S. J., Park, S., Nguyen, L. T., Hwang, J., Lee, E. Y., Giong, H. K., ... & Kim, S. (2019). A threonyl-tRNA synthetase-mediated translation initiation machinery. Nature communications, 10(1), 1357.
Recombinant Mouse Anti-AARS Recombinant Antibody (M6-P2E5) (CBMAB-XB0006-YC) in IF
Interactions between the indicated protein pairs determined by reconstitution of Venus green fluorescent protein (GFP) in Chinese hamster ovary (CHO) cells. Nuclei were stained with 4',6-diamidino-2 phenylindole (DAPI; blue). Expression of HA-4EHP and FLAG-TRS was confirmed by immunofluorescence staining with anti-HA (Alexa 647; purple) and anti-FLAG (Alexa 594; red) antibodies, respectively. Scale bar = 10 μM. ...View More
Jeong, S. J., Park, S., Nguyen, L. T., Hwang, J., Lee, E. Y., Giong, H. K., ... & Kim, S. (2019). A threonyl-tRNA synthetase-mediated translation initiation machinery. Nature communications, 10(1), 1357.

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-444421
Antibody Isotype
IgG1, κ
Application
ELISA, IHC, WB

Basic Information

Immunogen
Peptide-TSFAQLRLGD (amino acid 956-965)
Specificity
Human
Antibody Isotype
IgG1, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
ELISA1:30-1:3,000
IF(ICC)1:50-1:500
IP1-2 µl per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at-20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Alanyl-TRNA Synthetase
Introduction
The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.
Entrez Gene ID
16
UniProt ID
P49588
Alternative Names
Alanyl-TRNA Synthetase; Alanine TRNA Ligase 1, Cytoplasmic; Renal Carcinoma Antigen NY-REN-42; EC 6.1.1.7; AlaRS; Alanyl-TRNA Synthetase, Cytoplasmic;
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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