What species reactivity is guaranteed for the Rabbit Anti-AARSD1 Recombinant Antibody (CBMAB-EN004-LY)?

The Rabbit Anti-AARSD1 Recombinant Antibody (CBMAB-EN004-LY) is raised against human AARSD1 and is guaranteed to react with human samples. Based on sequence homology, it is also expected to react with AARSD1 from mouse and rat, and this cross-reactivity is often indicated on the product datasheet. We always recommend checking the most up-to-date product information on our website for confirmed species reactivity. For other species, evaluation with a trial size may be beneficial.

What type of positive control should be used for Western blotting with this antibody?

For Western blotting with the Rabbit Anti-AARSD1 Recombinant Antibody (CBMAB-EN004-LY), any cell line or tissue lysate known to endogenously express AARSD1 can serve as a positive control. HEK293T cells, HeLa cells, or mouse embryonic fibroblasts (MEFs) are commonly used as they typically express AARSD1. Alternatively, if available, a recombinant AARSD1 protein can be used as a positive control to confirm the antibody's ability to recognize the target.

Rabbit Anti-AARSD1 Recombinant Antibody (V2-639277) (CBMAB-EN004-LY)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

V2-639277

Application

WB: 1:500~1:1000 ELISA: 1:5000

Immunogen

The antibody was produced against synthesized peptide derived from internal of human AARSD1.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Alanyl-TRNA Synthetase Domain Containing 1

Introduction

Functions in trans to edit the amino acid moiety from incorrectly charged tRNA(Ala).

Entrez Gene ID

Human	80755
Mouse	69684
Rat	619440

UniProt ID

Human	Q9BTE6
Mouse	Q3THG9
Rat	Q5XI97

Alternative Names

Alanyl-TRNA Synthetase Domain Containing 1; Alanyl-TRNA Synthetase Domain-Containing Protein 1;

Function

Functions in trans to edit the amino acid moiety from incorrectly charged tRNA(Ala).

Biological Process

Alanyl-tRNA aminoacylation
Regulation of translational fidelity

Cellular Location

Cytoplasm

Elshiekh, A. A., Albkrye, A. M., Khaeir, M. A. M., & Elnasri, H. A. (2020). Analysis of Single Nucleotide Polymorphisms in Human Alanyl-tRNA Synthetase (AARS) Gene: an in Silico Approach.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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