Mouse Anti-ABCA7 Recombinant Antibody (V2-12489) (CBMAB-0979-CN)

Name: Mouse Anti-ABCA7 Recombinant Antibody (V2-12489)
SKU: CBMAB-0979-CN
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-12489

Application

WB, ELISA, IF, IHC-P, IP

Immunogen

Raised against amino acids 2009-2146 mapping at the C-terminus of ABCA7 of human origin

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
ELISA	1:30-1:3,000
IF(ICC)	1:50-1:500
IP	1-2 µl per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ATP Binding Cassette Subfamily A Member 7

Introduction

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This full transporter has been detected predominantly in myelo-lymphatic tissues with the highest expression in peripheral leukocytes, thymus, spleen, and bone marrow. The function of this protein is not yet known; however, the expression pattern suggests a role in lipid homeostasis in cells of the immune system. The protein plays a role in phagocytosis by macrophages of apoptotic cells. Binds APOA1 and may function in apolipoprotein-mediated phospholipid efflux from cells. It may also mediate cholesterol efflux and regulate cellular ceramide homeostasis during keratinocytes differentiation.

Entrez Gene ID

10347

UniProt ID

Q8IZY2

Alternative Names

AD9; ABCX; ABCA-SSN

Function

Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP. Transports preferentially phosphatidylserine over phosphatidylcholine. Plays a role in lipid homeostasis and macrophage-mediated phagocytosis. Binds APOA1 and may function in apolipoprotein-mediated phospholipid efflux from cells. May also mediate cholesterol efflux. May regulate cellular ceramide homeostasis during keratinocyte differentiation. Involved in lipid raft organization and CD1D localization on thymocytes and antigen-presenting cells, which plays an important role in natural killer T-cell development and activation (By similarity). Plays a role in phagocytosis of apoptotic cells by macrophages (By similarity). Macrophage phagocytosis is stimulated by APOA1 or APOA2, probably by stabilization of ABCA7 (By similarity). Also involved in phagocytic clearance of amyloid-beta by microglia cells and macrophages (By similarity). Further limits amyloid-beta production by playing a role in the regulation of amyloid-beta A4 precursor protein (APP) endocytosis and/or processing. Amyloid-beta is the main component of amyloid plaques found in the brains of Alzheimer patients.

Biological Process

Amyloid-beta clearance by cellular catabolic process
Amyloid-beta formation
Apolipoprotein A-I-mediated signaling pathway
Cholesterol efflux
High-density lipoprotein particle assembly
Lipid transport
Memory
Negative regulation of amyloid-beta formation
Negative regulation of amyloid precursor protein biosynthetic process
Negative regulation of endocytosis
Negative regulation of MAPK cascade
Negative regulation of PERK-mediated unfolded protein response
Peptide cross-linking
Phagocytosis
Phospholipid efflux
Phospholipid translocation
Plasma membrane raft organization
Positive regulation of amyloid-beta clearance
Positive regulation of cholesterol efflux
Positive regulation of engulfment of apoptotic cell
Positive regulation of ERK1 and ERK2 cascade
Positive regulation of phagocytosis
Positive regulation of phospholipid efflux
Positive regulation of protein localization to cell surface
Protein localization to nucleus
Regulation of amyloid precursor protein catabolic process
Regulation of lipid metabolic process
Visual learning

Cellular Location

Cytoplasm; Early endosome membrane; Cell membrane; Golgi apparatus membrane; Phagocytic cup. Localizes to cell membrane ruffles and phagocytic cups of macrophages stimulated with C1q or apoptotic cells. Localizes to the cytoplasm of resting macrophages, probably in Golgi and endosomes. Localizes to the apical brush border of cells in the proximal tubules of kidney (By similarity).

Involvement in disease

A familial, late-onset form of Alzheimer disease. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituents of these plaques are neurotoxic amyloid-beta protein 40 and amyloid-beta protein 42, that are produced by the proteolysis of the transmembrane APP protein. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products, such as C31, are also implicated in neuronal death.

Topology

Helical: 22-42 aa
Extracellular: 43-549 aa
Helical: 550-1263 aa
Extracellular: 1264-1537 aa
Helical: 1538-1749 aa

PTM

N-glycosylated.

Dib, S., Pahnke, J., & Gosselet, F. (2021). Role of ABCA7 in Human Health and in Alzheimer’s Disease. International Journal of Molecular Sciences, 22(9), 4603.

Aikawa, T., Ren, Y., Holm, M. L., Asmann, Y. W., Alam, A., Fitzgerald, M. L., ... & Kanekiyo, T. (2021). ABCA7 regulates brain fatty acid metabolism during LPS-induced acute inflammation. Frontiers in Neuroscience, 15.

Bossaerts, L., Hens, E., Hanseeuw, B., Vandenberghe, R., Cras, P., De Deyn, P. P., ... & BELNEU Consortium. (2021). Premature termination codon mutations in ABCA7 contribute to Alzheimer's disease risk in Belgian patients. Neurobiology of Aging.

Algahtani, H., Shirah, B., Alshareef, A., Al-Qahtani, M. H., Abdulkareem, A. A., & Naseer, M. I. (2020). A novel variant c. 3706C> T p.(Avg 1236Cys) in the ABCA7 gene in a Saudi patient with susceptibility to Alzheimer's disease 9. Intractable & Rare Diseases Research.

Fehér, Á., Juhász, A., Pákáski, M., Janka, Z., & Kálmán, J. (2019). Association study of the ABCA7 rs3752246 polymorphism in Alzheimer's disease. Psychiatry research, 279, 376-377.

De Roeck, A., Van Broeckhoven, C., & Sleegers, K. (2019). The role of ABCA7 in Alzheimer’s disease: evidence from genomics, transcriptomics and methylomics. Acta neuropathologica, 138(2), 201-220.

De Roeck, A., Van den Bossche, T., van der Zee, J., Verheijen, J., De Coster, W., Van Dongen, J., ... & Sleegers, K. (2017). Deleterious ABCA7 mutations and transcript rescue mechanisms in early onset Alzheimer’s disease. Acta neuropathologica, 134(3), 475-487.

Zhou, G., Mao, X., Chu, J., Chen, G., Zhao, Q., Wang, L., & Luo, Y. (2017). ATP binding cassette subfamily A member 7 rs3764650 polymorphism and the risk of Alzheimer's disease. Die Pharmazie-An International Journal of Pharmaceutical Sciences, 72(7), 425-427.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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