Is the Rat Anti-ABCC12 Recombinant Antibody suitable for both native and denatured protein detection?

Recombinant antibodies from Creative Biolabs are engineered for high specificity and can often recognize both native (conformational) and denatured (linear) epitopes, depending on the specific immunogen and clone. For applications like Western Blot, where proteins are denatured, the antibody is typically designed to recognize linear epitopes. For applications like flow cytometry or immunoprecipitation that deal with native protein structures, it would ideally recognize conformational epitopes. It is always recommended to check the product datasheet or consult Creative Biolabs' technical support for specific validation in different protein states, especially if working with native protein structures.

What is the recommended dilution range for this antibody, and are pre-trials necessary?

Creative Biolabs usually provides a recommended starting dilution range on the product datasheet for common applications (e.g., 1:500 - 1:2000 for WB, or 1-5 µg/mL for IHC). However, given the variability across experimental systems (e.g., sample type, detection method, substrate sensitivity), it is highly recommended to perform a preliminary titration experiment. This involves testing a range of dilutions around the recommended starting point to determine the optimal working concentration that yields the strongest specific signal with the lowest background for your unique experimental conditions.

Rat Anti-ABCC12 Recombinant Antibody (V2-179005) (CBMAB-A0240-YC)

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Published Data

Rat Anti-ABCC12 Recombinant Antibody (M9II-3) (CBMAB-A0240-YC) in IHC

The upper panel shows sections of mouse epididymis stained with the rat mAbs indicated. The control was treated only with the second antibody. The lower panel shows mouse sperm stained with the same set of antibodies. See the Materials and methods section for details. ...View More

Ono, N., Van der Heijden, I., Scheffer, G. L., Van de Wetering, K., Van Deemter, E., De Haas, M., ... & Borst, P. (2007). Multidrug resistance-associated protein 9 (ABCC12) is present in mouse and boar sperm. Biochemical Journal, 406(1), 31-40.

Rat Anti-ABCC12 Recombinant Antibody (M9II-3) (CBMAB-A0240-YC) in WB

Western blotting of ABCC2 in LoVo cells. ...View More

Liu, J., Qian, B., Zhou, L., Shen, G., Tan, Y., Liu, S., ... & Yang, Z. (2022). Il25 enhanced colitis-associated tumorigenesis in mice by upregulating transcription factor Gli1. Frontiers in Immunology, 13.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rat

Clone

V2-179005

Application

FC, ICC, IHC-Fr, WB

Immunogen

Recombinant protein corresponding to amino acids 690-734 of human ABCC12.

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

0.7% BSA, 0.1% sodium azide

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ATP BINDING CASSETTE SUBFAMILY C MEMBER 12

Introduction

ABCC12 belongs to the superfamily of ATP-binding cassette (ABC) transporters and the encoded protein contains two ATP-binding domains and 12 transmembrane regions. ABC proteins transport various molecules across extra- and intracellular membranes. ABC gen

Entrez Gene ID

94160

UniProt ID

Q96J65

Alternative Names

ATP Binding Cassette Subfamily C Member 12; ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 12; ATP-Binding Cassette Sub-Family C Member 12; MRP9; ATP-Binding Cassette Transporter Sub-Family C Member 12; Multidrug Resistance-Associated Protein 9;

Function

Probable transporter, its substrate specificity is unknown.

Biological Process

Transmembrane transport

Cellular Location

Endoplasmic reticulum membrane

Zhang, L., Huang, P., Huang, C., Jiang, L., Lu, Z., & Wang, P. (2021). Varied clinical significance of ATP-binding cassette C sub-family members for lung adenocarcinoma. Medicine, 100(16).

Fujishiro, H., & Himeno, S. (2019). Gene expression profiles of immortalized S1, S2, and S3 cells derived from each segment of mouse kidney proximal tubules. Fundamental Toxicological Sciences, 6(4), 117-123.

Mao, X., He, Z., Zhou, F., Huang, Y., & Zhu, G. (2019). Prognostic significance and molecular mechanisms of adenosine triphosphate-binding cassette subfamily C members in gastric cancer. Medicine, 98(50).

Tun-Yhong, W., Chinpaisal, C., Pamonsinlapatham, P., & Kaewkitichai, S. (2017). Tenofovir disoproxil fumarate is a new substrate of ATP-binding cassette subfamily C member 11. Antimicrobial agents and chemotherapy, 61(4), e01725-16.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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