What are the primary applications recommended for the Mouse Anti-ABCC6 Recombinant Antibody (V2-179037)?

This antibody is primarily recommended for Immunoprecipitation (IP), Immunohistochemistry (IHC) on paraffin-embedded tissues, and Western Blot (WB). Its recombinant nature ensures high specificity and consistency across these applications.

Is this antibody suitable for detecting endogenous levels of ABCC6, or is it better for overexpression systems?

The Mouse Anti-ABCC6 Recombinant Antibody (V2-179037) is designed to recognize endogenous levels of ABCC6. Its high affinity and specificity, characteristic of recombinant antibodies, make it suitable for detecting physiologically relevant expression levels in various biological samples, including those with endogenous expression. However, as with any antibody, signal intensity can vary with the abundance of the target protein, and optimization of experimental conditions may be necessary for low-expressing systems.

Mouse Anti-ABCC6 Recombinant Antibody (V2-179037) (CBMAB-A0273-YC)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

V2-179037

Application

ELISA, WB

Immunogen

ABCC6 (NP_001162, 831 a.a. ~ 930 a.a) partial recombinant protein with GST tag.

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.2

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ATP Binding Cassette Subfamily C Member 6

Introduction

ABCC6 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN

Entrez Gene ID

368

UniProt ID

O95255

Alternative Names

ATP Binding Cassette Subfamily C Member 6; ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 6; Anthracycline Resistance-Associated Protein; Multi-Specific Organic Anion Transporter E; MOAT-E; MRP6; ARA; ATP-Binding Cassette Sub-Family C Member 6; Mul

Function

Isoform 1: ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds, and xenobiotics from cells. Mediates ATP-dependent transport of glutathione conjugates such as leukotriene-c4 (LTC4) and N-ethylmaleimide S-glutathione (NEM-GS) (in vitro), and an anionic cyclopentapeptide endothelin antagonist, BQ-123. Does not appear to actively transport drugs outside the cell. Confers low levels of cellular resistance to etoposide, teniposide, anthracyclines and cisplatin.Mediates the release of nucleoside triphosphates, predominantly ATP, into the circulation, where it is rapidly converted into AMP and the mineralization inhibitor inorganic pyrophosphate (PPi) by the ecto-enzyme ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1), therefore playing a role in PPi homeostasis.
Isoform 2: Inhibits TNF-alpha-mediated apoptosis through blocking one or more caspases.

Biological Process

ATP transport
Cellular phosphate ion homeostasis
Drug transmembrane transport
Leukotriene transport
Response to drug
Transmembrane transport
Visual perception

Cellular Location

Isoform 1: Basolateral cell membrane
Isoform 2: Endoplasmic reticulum membrane

Involvement in disease

A multisystem disorder characterized by accumulation of mineralized and fragmented elastic fibers in the skin, vascular walls, and Burch membrane in the eye. Clinically, patients exhibit characteristic lesions of the posterior segment of the eye including peau d'orange, angioid streaks, and choroidal neovascularizations, of the skin including soft, ivory colored papules in a reticular pattern that predominantly affect the neck and large flexor surfaces, and of the cardiovascular system with peripheral and coronary arterial occlusive disease as well as gastrointestinal bleedings.
A severe autosomal recessive disorder characterized by calcification of the internal elastic lamina of muscular arteries and stenosis due to myointimal proliferation. The disorder is often fatal within the first 6 months of life because of myocardial ischemia resulting in refractory heart failure.

Topology

Extracellular: 1-31 aa
Helical: 32-52 aa
Cytoplasmic: 53-72 aa
Helical: 73-93 aa
Extracellular: 94-98 aa
Helical: 99-119 aa
Cytoplasmic: 120-131 aa
Helical: 132-149 aa
Extracellular: 150-167 aa
Helical: 168-188 aa
Cytoplasmic: 189-302 aa
Helical: 303-323 aa
Extracellular: 324-349 aa
Helical: 350-370 aa
Cytoplasmic: 371-426 aa
Helical: 427-447 aa
Extracellular: 448-450 aa
Helical: 451-471 aa
Cytoplasmic: 472-533 aa
Helical: 534-554 aa
Extracellular: 555-575 aa
Helical: 576-596 aa
Cytoplasmic: 597-939 aa
Helical: 940-960 aa
Extracellular: 961-997 aa
Helical: 998-1018 aa
Cytoplasmic: 1019-1061 aa
Helical: 1062-1082 aa
Extracellular: 1083 aa
Helical: 1084-1104 aa
Cytoplasmic: 1105-1175 aa
Helical: 1176-1196 aa
Extracellular: 1197-1198 aa
Helical: 1199-1219 aa
Cytoplasmic: 1220-1503 aa

PTM

Glycosylated.

Szeri, F., Corradi, V., Niaziorimi, F., Donnelly, S., Conseil, G., Cole, S. P., ... & van de Wetering, K. (2021). Mutagenic Analysis of the Putative ABCC6 Substrate-Binding Cavity Using a New Homology Model. International Journal of Molecular Sciences, 22(13), 6910.

Bisaccia, F., Koshal, P., Abruzzese, V., Castiglione Morelli, M. A., & Ostuni, A. (2021). Structural and Functional Characterization of the ABCC6 Transporter in Hepatic Cells: Role on PXE, Cancer Therapy and Drug Resistance. International Journal of Molecular Sciences, 22(6), 2858.

Verschuere, S., Van Gils, M., Nollet, L., & Vanakker, O. M. (2020). From membrane to mineralization: the curious case of the ABCC6 transporter. FEBS letters, 594(23), 4109-4133.

Tiemann, J., Wagner, T., Lindenkamp, C., Plümers, R., Faust, I., Knabbe, C., & Hendig, D. (2020). Linking ABCC6 Deficiency in Primary Human Dermal Fibroblasts of PXE Patients to p21-Mediated Premature Cellular Senescence and the Development of a Proinflammatory Secretory Phenotype. International Journal of Molecular Sciences, 21(24), 9665.

De Vilder, E. Y., Cardoen, S., Hosen, M. J., Le Saux, O., De Zaeytijd, J., Leroy, B. P., ... & Vanakker, O. M. (2018). Pathogenic variants in the ABCC6 gene are associated with an increased risk for ischemic stroke. Brain Pathology, 28(6), 822-831.

Martinelli, F., Cuviello, F., Pace, M. C., Armentano, M. F., Miglionico, R., Ostuni, A., & Bisaccia, F. (2018). Extracellular ATP regulates CD73 and ABCC6 expression in HepG2 cells. Frontiers in molecular biosciences, 5, 75.

Akhter, S., Georgas, T., & Giannoukos, G. (2018). Investigating Mutations in Member 6 (ABCC6) gene, of Sub-Family C (CFTR/MRP) of the ATP-binding Cassette Causing Pseudoxanthoma elasficum. J Biol Med Res, 2(2), 17.

Colleoni, L., Galbardi, B., Barzago, C., Bonanno, S., Franzi, S., Frangiamore, R., ... & Kapetis, D. (2017). A novel ABCC6 haplotype is associated with azathioprine drug response in myasthenia gravis. Pharmacogenetics and genomics, 27(2), 51-56.

Miglionico, R., Ostuni, A., Armentano, M. F., Milella, L., Crescenzi, E., Carmosino, M., & Bisaccia, F. (2017). ABCC6 knockdown in HepG2 cells induces a senescent-like cell phenotype. Cellular & molecular biology letters, 22(1), 1-10.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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