Is this antibody suitable for detecting ABCC7 in tissue sections, such as for Immunohistochemistry (IHC)?

While the product page does not explicitly list IHC as a validated application, recombinant antibodies often perform well in IHC due to their high specificity. If you plan to use it for IHC, we recommend starting with optimization steps, including antigen retrieval methods (e.g., heat-induced epitope retrieval with citrate or Tris-EDTA buffer) and antibody titration, to determine its suitability and optimal conditions for your specific tissue type and preparation.

What are the recommended controls for Western Blot experiments using this antibody?

For Western Blot, it is highly recommended to include a positive control (e.g., cell lysates known to express ABCC7) and a negative control (e.g., cell lysates not expressing ABCC7 or a knockout/knockdown sample if available). An isotype control should also be considered if you are troubleshooting non-specific bands. Loading controls (e.g., GAPDH, Actin) are essential to ensure equal protein loading across lanes.

Mouse Anti-ABCC7 Recombinant Antibody (V2-179042) (CBMAB-A0278-YC)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

V2-179042

Application

IHC-P

Immunogen

Recombinant protein encoding NBF2 domain of human CFTR

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

0.1 M Tris-glycine, pH7.4, 30% glycerol

Preservative

0.05% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

cystic fibrosis transmembrane conductance regulator

Introduction

ABCC7 is a member of the ATP-binding cassette (ABC) transporter superfamily. The encoded protein functions as a chloride channel, making it unique among members of this protein family, and controls ion and water secretion and absorption in epithelial tiss

Entrez Gene ID

1080

UniProt ID

P13569

Alternative Names

CF; MRP7; ABC35; ABCC7; CFTR/MRP; TNR-CFTR; dJ760C5.1; cystic fibrosis transmembrane conductance regulator; cAMP-dependent chloride channel; channel conductance-controlling ATPase; cystic fibrosis transmembrane conductance regulating; cystic fibrosis tran

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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