Mouse Anti-ABCF2 Recombinant Antibody (V2-179071) (CBMAB-A0307-YC)

Name: Mouse Anti-ABCF2 Recombinant Antibody (V2-179071)
SKU: CBMAB-A0307-YC
Rating: 5 (1 reviews)

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Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-179071

Antibody Isotype

IgG1

Application

Dot, FC, IP, WB

Basic Information

Immunogen

Raised against a recombinant protein corresponding to an internal region of ABCF2 of human origin

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

PBS, pH 7.4, 1% BSA, 0.05% sodium azide

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

ATP Binding Cassette Subfamily F Member 2

Introduction

ABCF2 is a member of the ATP-binding cassette (ABC) transporter superfamily. ATP-binding cassette proteins transport various molecules across extra- and intracellular membranes. Alterations in this gene may be involved in cancer progression. Related pseud

Entrez Gene ID

10061

UniProt ID

Q9UG63

Alternative Names

ATP Binding Cassette Subfamily F Member 2; ATP-Binding Cassette, Sub-Family F (GCN20), Member 2; Iron-Inhibited ABC Transporter 2; ATP-Binding Cassette Sub-Family F Member 2; ABC-Type Transport Protein; EC 3.6.3.17;

Cellular Location

Membrane

More Infomation

References

Barrera, G., Cucci, M. A., Grattarola, M., & Pizzimenti, S. (2021). Nrf2, YAP, antioxidant potential, and cancer. In Cancer (pp. 159-170). Academic Press.

Gao, J., Dai, C., Yu, X., Yin, X. B., & Zhou, F. (2020). Circ‐TCF4. 85 silencing inhibits cancer progression through microRNA‐486‐5p‐targeted inhibition of ABCF2 in hepatocellular carcinoma. Molecular oncology, 14(2), 447-461.

Duz, M. B., & Karatas, O. F. (2020). Expression profile of stem cell markers and ABC transporters in 5-fluorouracil resistant Hep-2 cells. Molecular Biology Reports, 47(7), 5431-5438.

Xiao, S., Zhang, M., Liu, C., & Wang, D. (2018). MiR-514 attenuates proliferation and increases chemoresistance by targeting ATP binding cassette subfamily in ovarian cancer. Molecular Genetics and Genomics, 293(5), 1159-1167.

Bao, L., Wu, J., Dodson, M., Rojo de la Vega, E. M., Ning, Y., Zhang, Z., ... & Yi, X. (2017). ABCF2, an Nrf2 target gene, contributes to cisplatin resistance in ovarian cancer cells. Molecular carcinogenesis, 56(6), 1543-1553.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-ABCF2 Recombinant Antibody (V2-179070)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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Documents

Datasheet
SDS
Request for COA

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Second antibody and isotype control

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