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Mouse Anti-ABCG2 Recombinant Antibody (V2-12492) (CBMAB-0982-CN)

This product is a mouse antibody that recognizes ABCG2 of human. The antibody CBLNA-165 can be used for immunoassay techniques such as: WB, IHC, ICC, IP.
See all ABCG2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-12492
Antibody Isotype
IgG
Application
WB, IHC, ICC, IP

Basic Information

Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:500-1:5,000
IHC1:50-1:200
IF(ICC)1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH7.4, 50% Glycerol
Preservative
0.02% sodium azide
Concentration
1 mg/ml
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATP-binding cassette, sub-family G (WHITE), member 2
Introduction
The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue.
Entrez Gene ID
9429
UniProt ID
Q9UNQ0
Alternative Names
MRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CD338; GOUT1; MXR-1; CDw338; UAQTL1; EST157481
Function
Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes a wide variety of physiological compounds, dietary toxins and xenobiotics from cells. Involved in porphyrin homeostasis, mediating the export of protoporphyrin IX (PPIX) from both mitochondria to cytosol and cytosol to extracellular space, it also functions in the cellular export of heme. Also mediates the efflux of sphingosine-1-P from cells. Acts as a urate exporter functioning in both renal and extrarenal urate excretion. In kidney, it also functions as a physiological exporter of the uremic toxin indoxyl sulfate (By similarity). Also involved in the excretion of steroids like estrone 3-sulfate/E1S, 3beta-sulfooxy-androst-5-en-17-one/DHEAS, and other sulfate conjugates. Mediates the secretion of the riboflavin and biotin vitamins into milk (By similarity). Extrudes pheophorbide a, a phototoxic porphyrin catabolite of chlorophyll, reducing its bioavailability (By similarity). Plays an important role in the exclusion of xenobiotics from the brain (Probable). It confers to cells a resistance to multiple drugs and other xenobiotics including mitoxantrone, pheophorbide, camptothecin, methotrexate, azidothymidine, and the anthracyclines daunorubicin and doxorubicin, through the control of their efflux. In placenta, it limits the penetration of drugs from the maternal plasma into the fetus (By similarity). May play a role in early stem cell self-renewal by blocking differentiation (By similarity).
Biological Process
Biotin transport
Cellular detoxification
Cellular iron ion homeostasis
Export across plasma membrane
Heme biosynthetic process
Heme catabolic process
Organic anion transport
Riboflavin transport
Transepithelial transport
Transmembrane transport
Transport across blood-brain barrier
Urate metabolic process
Urate salt excretion
Xenobiotic transport across blood-brain barrier
Cellular Location
Cell membrane; Apical cell membrane; Mitochondrion membrane. Enriched in membrane lipid rafts.
Topology
Cytoplasmic: 1-395 aa
Helical: 396-416 aa
Extracellular: 417-428 aa
Helical: 429-449 aa
Cytoplasmic: 450-477 aa
Helical: 478-498 aa
Extracellular: 499-506 aa
Helical: 507-527 aa
Cytoplasmic: 528-535 aa
Helical: 536-556 aa
Extracellular: 557-630 aa
Helical: 631-651 aa
Cytoplasmic: 652-655 aa
PTM
N-glycosylated. Glycosylation-deficient ABCG2 is normally expressed and functional.
Phosphorylated. Phosphorylation at Thr-362 by PIM1 is induced by drugs like mitoxantrone and is associated with cells increased drug resistance. It regulates the localization to the plasma membrane, the homooligomerization and therefore, the activity of the transporter.

Wu, S., Zhang, X., Dong, M., Yang, Z., Zhang, M., & Chen, Q. (2020). sATP‑binding cassette subfamily G member 2 enhances the multidrug resistance properties of human nasal natural killer/T cell lymphoma side population cells. Oncology reports, 44(4), 1467-1478.

Ma, S., Liu, H., Sun, W., Mustafa, A., Xi, Y., Pu, F., ... & Hua, H. (2020). Molecular evolution of the ATP-binding cassette subfamily G member 2 gene subfamily and its paralogs in birds. BMC evolutionary biology, 20(1), 1-14.

Xing, J. Y., Zhang, Y. J., Jing, W. Q., Li, W. L., & Yu, Y. M. (2019). MOLECULAR CHARACTERIZATION, EXPRESSION PATTERNS AND METHYLATION ANALYSIS OF ATP-BINDING CASSETTE SUB-FAMILY G MEMBER 2 (ABCG2) IN BROILERS. JAPS, Journal of Animal and Plant Sciences, 29(1), 16-24.

Arumugam, A., Subramani, R., Nandy, S. B., Terreros, D., Dwivedi, A. K., Saltzstein, E., & Lakshmanaswamy, R. (2019). Silencing growth hormone receptor inhibits estrogen receptor negative breast cancer through ATP-binding cassette sub-family G member 2. Experimental & molecular medicine, 51(1), 1-13.

Liu, H., Song, X., Hou, J., Zhao, Z., & Chang, J. (2018). Posttranscriptional regulation of human antigen R by miR-133b enhances docetaxel cytotoxicity through the inhibition of ATP-binding cassette subfamily G member 2 in prostate cancer cells. DNA and cell biology, 37(3), 210-219.

Zhang, Z., Guo, X., To, K. K., Chen, Z., Fang, X., Luo, M., ... & Fu, L. (2018). Olmutinib (HM61713) reversed multidrug resistance by inhibiting the activity of ATP-binding cassette subfamily G member 2 in vitro and in vivo. Acta pharmaceutica sinica B, 8(4), 563-574.

Xu, L., Li, S., Liang, Z., Lin, H., & Fu, R. (2018). Acridone suppresses the proliferation of human breast cancer cells in vitro via ATP-binding cassette subfamily G member 2. Oncology letters, 15(2), 2651-2654.

Zhang, G. N., Zhang, Y. K., Wang, Y. J., Barbuti, A. M., Zhu, X. J., Yu, X. Y., ... & Chen, Z. S. (2017). Modulating the function of ATP-binding cassette subfamily G member 2 (ABCG2) with inhibitor cabozantinib. Pharmacological research, 119, 89-98.

Wu, C. P., Murakami, M., Hsiao, S. H., Chou, A. W., Li, Y. Q., Huang, Y. H., ... & Ambudkar, S. V. (2017). Overexpression of ATP-binding cassette subfamily G member 2 confers resistance to phosphatidylinositol 3-kinase inhibitor PF-4989216 in cancer cells. Molecular pharmaceutics, 14(7), 2368-2377.

Toyoki, D., Shibata, S., Kuribayashi-Okuma, E., Xu, N., Ishizawa, K., Hosoyamada, M., & Uchida, S. (2017). Insulin stimulates uric acid reabsorption via regulating urate transporter 1 and ATP-binding cassette subfamily G member 2. American Journal of Physiology-Renal Physiology, 313(3), F826-F834.

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For research use only. Not intended for any clinical use.

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