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Mouse Anti-ABCG8 Recombinant Antibody (V2-179094) (CBMAB-A0331-YC)

Provided herein is a Mouse monoclonal antibody against Human ATP Binding Cassette Subfamily G Member 8. The antibody can be used for immunoassay techniques, such as WB, IHC, ICC, IF, IHC-P.
See all ABCG8 antibodies
Published Data
Fig.1 Subcellular localization and expression of ABCG8 and their allelic variants in immunohistochemistry analysis in HEK cells.
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Fig.2 Western blot analysis to confirm the present of ABCG5 and ABCG8.
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Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
V2-179094
Antibody Isotype
IgG
Application
WB, IHC, ICC, IF, IHC-P

Basic Information

Immunogen
Full length mouse ABCG8
Specificity
Human, Mouse
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IHC-P1:100
IHC1:100
IP25 µg
IF(ICC)1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
Tris-Glycine
Preservative
0.05% sodium azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ATP Binding Cassette Subfamily G Member 8
Introduction
ABCG8 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN
Entrez Gene ID
Human64241
Mouse67470
UniProt ID
HumanQ9H221
MouseQ9DBM0
Alternative Names
ATP Binding Cassette Subfamily G Member 8; ATP-Binding Cassette, Sub-Family G (WHITE), Member 8; Sterolin 2; ATP-Binding Cassette, Sub-Family G (WHITE), Member 8 (Sterolin 2); ATP-Binding Cassette Sub-Family G Member 8; Gallbladder Disease 4; Sterolin-2;
Function
ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg2+- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG5 has ATPase activity.
Biological Process
Bile acid signaling pathway
Cholesterol efflux
Cholesterol homeostasis
Excretion
Intestinal cholesterol absorption
Intracellular receptor signaling pathway
Lipid transport
Negative regulation of intestinal cholesterol absorption
Negative regulation of intestinal phytosterol absorption
Phospholipid transport
Response to drug
Response to muscle activity
Response to nutrient
Sterol transport
Triglyceride homeostasis
Cellular Location
Cell membrane; Apical cell membrane
Involvement in disease
One of the major digestive diseases. Gallstones composed of cholesterol (cholelithiasis) are the common manifestations in western countries. Most people with gallstones, however, remain asymptomatic through their lifetimes.
A form of sitosterolemia, an autosomal recessive metabolic disorder characterized by unregulated intestinal absorption of cholesterol, phytosterols and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease.
Topology
Cytoplasmic: 1-416 aa
Helical: 417-437 aa
Extracellular: 438-447 aa
Helical: 448-468 aa
Cytoplasmic: 469-497 aa
Helical: 498-518 aa
Extracellular: 519-527 aa
Helical: 528-548 aa
Cytoplasmic: 549-555 aa
Helical: 556-576 aa
Extracellular: 577-639 aa
Helical: 640-660 aa
Cytoplasmic: 661-673 aa
PTM
N-glycosylated.

Fashe, M., Yi, M., Sueyoshi, T., & Negishi, M. (2021). Sex-specific expression mechanism of hepatic estrogen inactivating enzyme and transporters in diabetic women. Biochemical Pharmacology, 114662.

Fan, N., Meng, K., Zhang, Y., Hu, Y., Li, D., Gao, Q., ... & Cui, Y. (2020). The effect of ursodeoxycholic acid on the relative expression of the lipid metabolism genes in mouse cholesterol gallstone models. Lipids in Health and Disease, 19(1), 1-11.

Bastida, J. M., Benito, R., González-Porras, J. R., & Rivera, J. (2020). ABCG5 and ABCG8 gene variations associated with sitosterolemia and platelet dysfunction. Platelets, 1-5.

Mikhailova, S., Ivanoshchuk, D., Timoshchenko, O., & Shakhtshneider, E. (2019). Genes potentially associated with familial hypercholesterolemia. Biomolecules, 9(12), 807.

Zheng, J., Ma, J., Wu, R. H., Zhang, X., Su, Y., Zhang, R., & Zhang, L. Q. (2019). Unusual presentations of sitosterolemia limited to hematological abnormalities: a report of four cases presenting with stomatocytic anemia and thrombocytopenia with macrothrombocytes. American journal of hematology, 94(5), E124-E127.

Frigerio, P., Cepeda-Nieto, A. C., Marcos-Morales, S., Peña-Velázquez, A., Dávila-Flores, S., & Salinas-Santander, M. (2018). Distribution of the ATP-binding cassette transporter ABCG8 IVS1-2A> G genotype and clinical characteristics of gallbladder patients in Northeastern Mexico: A pilot study. Biomedical reports, 9(3), 266-270.

Tada, H., Nomura, A., Yamagishi, M., & Kawashiri, M. A. (2018). First case of sitosterolemia caused by double heterozygous mutations in ABCG5 and ABCG8 genes. Journal of clinical lipidology, 12(5), 1164-1168.

Tada, H., Nomura, A., Nohara, A., Inazu, A., Mabuchi, H., Yamagishi, M., & Kawashiri, M. A. (2018). Post-prandial remnant lipoprotein metabolism in sitosterolemia. Journal of atherosclerosis and thrombosis, 25(12), 1188-1195.

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For research use only. Not intended for any clinical use.

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