What is the recommended dilution range for this antibody in Western Blotting?

Based on my experience and typical antibody recommendations, a dilution range of 1:1000 to 1:2000 is a good starting point for Western Blotting with this antibody. However, as with all antibodies, optimizing the dilution for your specific experimental conditions (sample type, protein concentration, detection system) is always recommended to achieve the best signal-to-noise ratio.

Can this antibody be used to detect ABCG8 in species other than mouse?

While this specific antibody is a mouse recombinant antibody targeting ABCG8, its reactivity with other species largely depends on the sequence homology of ABCG8 across species and the specific immunogen used. Always check the product datasheet for validated species reactivity. If your species of interest isn't listed, you might consider performing a sequence alignment of your target species' ABCG8 with the immunogen sequence, or contacting Creative Biolabs directly for cross-reactivity information or to inquire about a trial size.

Mouse Anti-ABCG8 Recombinant Antibody (V2-179094) (CBMAB-A0331-YC)

See all ABCG8 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179094

Application

WB, IHC, ICC, IF, IHC-P

Immunogen

Full length mouse ABCG8

Specificity

Human, Mouse

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IHC-P	1:100
IHC	1:100
IP	25 µg
IF(ICC)	1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

Tris-Glycine

Preservative

0.05% sodium azide

Concentration

1 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ATP Binding Cassette Subfamily G Member 8

Introduction

ABCG8 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN

Entrez Gene ID

Human	64241
Mouse	67470

UniProt ID

Human	Q9H221
Mouse	Q9DBM0

Alternative Names

ATP Binding Cassette Subfamily G Member 8; ATP-Binding Cassette, Sub-Family G (WHITE), Member 8; Sterolin 2; ATP-Binding Cassette, Sub-Family G (WHITE), Member 8 (Sterolin 2); ATP-Binding Cassette Sub-Family G Member 8; Gallbladder Disease 4; Sterolin-2;

Function

ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg2+- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG5 has ATPase activity.

Biological Process

Bile acid signaling pathway
Cholesterol efflux
Cholesterol homeostasis
Excretion
Intestinal cholesterol absorption
Intracellular receptor signaling pathway
Lipid transport
Negative regulation of intestinal cholesterol absorption
Negative regulation of intestinal phytosterol absorption
Phospholipid transport
Response to drug
Response to muscle activity
Response to nutrient
Sterol transport
Triglyceride homeostasis

Cellular Location

Cell membrane; Apical cell membrane

Involvement in disease

One of the major digestive diseases. Gallstones composed of cholesterol (cholelithiasis) are the common manifestations in western countries. Most people with gallstones, however, remain asymptomatic through their lifetimes.
A form of sitosterolemia, an autosomal recessive metabolic disorder characterized by unregulated intestinal absorption of cholesterol, phytosterols and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease.

Topology

Cytoplasmic: 1-416 aa
Helical: 417-437 aa
Extracellular: 438-447 aa
Helical: 448-468 aa
Cytoplasmic: 469-497 aa
Helical: 498-518 aa
Extracellular: 519-527 aa
Helical: 528-548 aa
Cytoplasmic: 549-555 aa
Helical: 556-576 aa
Extracellular: 577-639 aa
Helical: 640-660 aa
Cytoplasmic: 661-673 aa

PTM

N-glycosylated.

Fashe, M., Yi, M., Sueyoshi, T., & Negishi, M. (2021). Sex-specific expression mechanism of hepatic estrogen inactivating enzyme and transporters in diabetic women. Biochemical Pharmacology, 114662.

Fan, N., Meng, K., Zhang, Y., Hu, Y., Li, D., Gao, Q., ... & Cui, Y. (2020). The effect of ursodeoxycholic acid on the relative expression of the lipid metabolism genes in mouse cholesterol gallstone models. Lipids in Health and Disease, 19(1), 1-11.

Bastida, J. M., Benito, R., González-Porras, J. R., & Rivera, J. (2020). ABCG5 and ABCG8 gene variations associated with sitosterolemia and platelet dysfunction. Platelets, 1-5.

Mikhailova, S., Ivanoshchuk, D., Timoshchenko, O., & Shakhtshneider, E. (2019). Genes potentially associated with familial hypercholesterolemia. Biomolecules, 9(12), 807.

Zheng, J., Ma, J., Wu, R. H., Zhang, X., Su, Y., Zhang, R., & Zhang, L. Q. (2019). Unusual presentations of sitosterolemia limited to hematological abnormalities: a report of four cases presenting with stomatocytic anemia and thrombocytopenia with macrothrombocytes. American journal of hematology, 94(5), E124-E127.

Frigerio, P., Cepeda-Nieto, A. C., Marcos-Morales, S., Peña-Velázquez, A., Dávila-Flores, S., & Salinas-Santander, M. (2018). Distribution of the ATP-binding cassette transporter ABCG8 IVS1-2A> G genotype and clinical characteristics of gallbladder patients in Northeastern Mexico: A pilot study. Biomedical reports, 9(3), 266-270.

Tada, H., Nomura, A., Yamagishi, M., & Kawashiri, M. A. (2018). First case of sitosterolemia caused by double heterozygous mutations in ABCG5 and ABCG8 genes. Journal of clinical lipidology, 12(5), 1164-1168.

Tada, H., Nomura, A., Nohara, A., Inazu, A., Mabuchi, H., Yamagishi, M., & Kawashiri, M. A. (2018). Post-prandial remnant lipoprotein metabolism in sitosterolemia. Journal of atherosclerosis and thrombosis, 25(12), 1188-1195.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-ABCG8 Recombinant Antibody (V2-179094)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry