Mouse Anti-ABHD5 Recombinant Antibody (V2-179096) (CBMAB-A0337-YC)

Name: Mouse Anti-ABHD5 Recombinant Antibody (V2-179096)
SKU: CBMAB-A0337-YC
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ABHD5 Recombinant Antibody (4B12) (CBMAB-A0337-YC) in WB

Markers of lipolysis at rest and during exercise in HSL/ mice. CGI-58, comparative gene identification. ...View More

Huijsman, E., van de Par, C., Economou, C., van der Poel, C., Lynch, G. S., Schoiswohl, G., ... & Watt, M. J. (2009). Adipose triacylglycerol lipase deletion alters whole body energy metabolism and impairs exercise performance in mice. American Journal of Physiology-Endocrinology and Metabolism, 297(2), E505-E513.

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179096

Application

ELISA, WB

Immunogen

ABHD5 (NP_057090, 240-342 aa) partial recombinant protein with GST tag. Immunogen sequence: FEDDTVTEYI YHCNVQTPSG ETAFKNMTIP YGWAKRPMLQ RIGKMHPDIP VSVIFGARSC IDGNSGTSIQ SLRPHSYVKT IAILGAGHYV YADQPEEFNQ KVK

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

aa 240-342

More Infomation

Target

Full Name

abhydrolase domain containing 5

Introduction

ABHD5 belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfami

Entrez Gene ID

51099

UniProt ID

Q8WTS1

Alternative Names

Abhydrolase Domain Containing 5; Abhydrolase Domain-Containing Protein 5; Lipid Droplet-Binding Protein CGI-58; EC 2.3.1.51; NCIE2; 1-Acylglycerol-3-Phosphate O-Acyltransferase ABHD5;

Function

Coenzyme A-dependent lysophosphatidic acid acyltransferase that catalyzes the transfert of an acyl group on a lysophosphatidic acid. Functions preferentially with 1-oleoyl-lysophosphatidic acid followed by 1-palmitoyl-lysophosphatidic acid, 1-stearoyl-lysophosphatidic acid and 1-arachidonoyl-lysophosphatidic acid as lipid acceptor. Functions preferentially with arachidonoyl-CoA followed by oleoyl-CoA as acyl group donors (By similarity). Functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation. Regulates lipid droplet fusion (By similarity).

Biological Process

Cell differentiation
Fatty acid metabolic process
Lipid homeostasis
Negative regulation of sequestering of triglyceride
Phosphatidic acid biosynthetic process
Positive regulation of lipoprotein lipase activity
Positive regulation of triglyceride catabolic process

Cellular Location

Cytoplasm; Cytosol; Lipid droplet. Colocalized with PLIN and ADRP on the surface of lipid droplets. The localization is dependent upon the metabolic status of the adipocytes and the activity of PKA (By similarity).

Involvement in disease

An autosomal recessive inborn error of lipid metabolism with multisystemic accumulation of triglycerides although plasma concentrations are normal. Clinical characteristics are congenital generalized ichthyosis, vacuolated leukocytes, hepatomegaly, myopathy, cataracts, neurosensory hearing loss and developmental delay. The disorder presents at birth with generalized, fine, white scaling of the skin and a variable degree of erythema resembling non-bullous congenital ichthyosiform erythroderma.

Peng, H. Y., Li, H. P., & Li, M. Q. (2020). Downregulated ABHD5 aggravates insulin resistance of trophoblast cells during gestational diabetes mellitus. Reproductive Sciences, 27(1), 233-245.

Liang, M., Chen, X., Wang, L., Qin, L., Wang, H., Sun, Z., ... & Geng, B. (2020). Cancer-derived exosomal TRIM59 regulates macrophage NLRP3 inflammasome activation to promote lung cancer progression. Journal of Experimental & Clinical Cancer Research, 39(1), 1-17.

Shang, S., Ji, X., Zhang, L., Chen, J., Li, C., Shi, R., ... & Miao, H. (2019). Macrophage ABHD5 suppresses NFκB-Dependent matrix metalloproteinase expression and cancer metastasis. Cancer research, 79(21), 5513-5526.

Jebessa, Z. H., Shanmukha, K. D., Dewenter, M., Lehmann, L. H., Xu, C., Schreiter, F., ... & Backs, J. (2019). The lipid-droplet-associated protein ABHD5 protects the heart through proteolysis of HDAC4. Nature metabolism, 1(11), 1157-1167.

Zhou, Q., Wang, F., Zhou, K., Huang, K., Zhu, Q., Luo, X., ... & Shi, Z. (2019). Oncogenic role of ABHD5 in endometrial cancer. Cancer management and research, 11, 2139.

Wang, X., Khan, R., Raza, S. H. A., Li, A., Zhang, Y., Liang, C., ... & Zan, L. (2019). Molecular characterization of ABHD5 gene promoter in intramuscular preadipocytes of Qinchuan cattle: Roles of Evi1 and C/EBPα. Gene, 690, 38-47.

Eskiocak, A. H., Missaglia, S., Moro, L., Durdu, M., & Tavian, D. (2019). A novel mutation of ABHD5 gene in a Chanarin Dorfman patient with unusual dermatological findings. Lipids in health and disease, 18(1), 1-6.

Lee, H. J., & Chang, B. (2017). The Roles of ABHD5 (Abhydrolase Domain Containing 5) in Plin2-Deficiency Mediated Autophagy Augmentation. Circulation, 136(suppl_1), A16203-A16203.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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