What applications is the Mouse Anti-ABTB1 Recombinant Antibody (V2-610904) validated for?

The Mouse Anti-ABTB1 Recombinant Antibody (V2-610904) is validated for sELISA, ELISA, and Immunofluorescence (IF(ICC)). For optimal results, users should determine the appropriate working dilutions and conditions for their specific experimental setup, referring to the Certificate of Analysis (COA) for recommended starting dilutions.

Can this antibody be used with samples from species other than human?

The specificity of this antibody is indicated for human ABTB1. While some antibodies may exhibit cross-reactivity with other species, it is not guaranteed. Creative Biolabs recommends contacting their scientists if you are interested in using this antibody with species or applications not listed on the datasheet. They may be able to provide recommendations or information on potential cross-reactivity.

Mouse Anti-ABTB1 Recombinant Antibody (V2-610904) (CBMAB-A0047-LY)

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Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-610904

Application

sELISA, ELISA

Immunogen

ABTB1 (NP_742024, 271 a.a. ~ 370 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
IF(ICC)	10 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ABTB1

Introduction

This gene encodes a protein with an ankyrin repeat region and two BTB/POZ domains, which are thought to be involved in protein-protein interactions. Expression of this gene is activated by the phosphatase and tensin homolog, a tumor suppressor. Alternate splicing results in three transcript variants encoding different isoforms. [provided by RefSeq]

Entrez Gene ID

80325

UniProt ID

Q969K4

Alternative Names

BPOZ; Btb3; EF1ABP; MGC20585; PP2259

Function

May act as a mediator of the PTEN growth-suppressive signaling pathway. May play a role in developmental processes.

Cellular Location

Cytoplasm

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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