What are the recommended applications for the Mouse Anti-ACAA1 Recombinant Antibody (V2-179142)?

This antibody is validated for a variety of immunoassay techniques, including Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC), and ELISA. Recommended starting dilutions for these applications are typically provided in the product's Certificate of Analysis (COA), but optimal dilutions should be determined by the end-user for their specific experimental conditions.

What species reactivity can I expect from the Mouse Anti-ACAA1 Recombinant Antibody (V2-179142)?

This particular antibody is specified to react with Human, Mouse, and Rat ACAA1. While cross-reactivity with other species is sometimes possible, it is not guaranteed unless explicitly stated. If you plan to use this antibody with samples from a species not listed, it is advisable to contact Creative Biolabs' technical support for potential recommendations or to consider performing preliminary validation experiments.

Mouse Anti-ACAA1 Recombinant Antibody (V2-179142) (CBMAB-A0393-YC)

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Published Data

Mouse Anti-ACAA1 Recombinant Antibody (2H6BE7) (CBMAB-A0393-YC) in WB

Western blot analysis of key proteins in fetal liver. ...View More

Xue, Y., Guo, C., Hu, F., Zhu, W., & Mao, S. (2019). Maternal undernutrition induces fetal hepatic lipid metabolism disorder and affects the development of fetal liver in a sheep model. The FASEB Journal, 33(9), 9990-10004.

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179142

Application

WB, IP, ICC, FC, ELISA

Immunogen

Tissue, cells or virus.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1 µg/ml
IF(ICC)	5 µg/ml
IP	5 µg/ml
FC	1 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

HEPES, pH 7.5

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

acetyl-Coenzyme A acyltransferase 1

Introduction

ACAA1 is an enzyme operative in the beta-oxidation system of the peroxisomes. Deficiency of this enzyme leads to pseudo-Zellweger syndrome.

Entrez Gene ID

Human	30
Mouse	113868
Rat	24157

UniProt ID

Human	P09110
Mouse	Q8VCH0
Rat	P21775

Alternative Names

Acetyl-CoA Acyltransferase 1; Peroxisomal 3-Oxoacyl-Coenzyme A Thiolase; Acetyl-Coenzyme A Acyltransferase 1; Peroxisomal 3-Oxoacyl-CoA Thiolase; Beta-Ketothiolase; EC 2.3.1.16; PTHIO;

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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