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Mouse Anti-ACAD11 Recombinant Antibody (V2-179169) (CBMAB-A0426-YC)

Provided herein is a Mouse monoclonal antibody against Human Acyl-CoA Dehydrogenase Family Member 11. The antibody can be used for immunoassay techniques, such as WB, IP, IF, ELISA.
See all ACAD11 antibodies
Published Data
Mouse Anti-ACAD11 Recombinant Antibody (B-12) (CBMAB-A0426-YC) in IF
Peroxisomal localization of endogenous ACAD11 in HepG2 cells. Cells were processed for immunofluorescence using anti-ACAD11 antibody. ...View More
Schrader, T. A., Islinger, M., & Schrader, M. (2017). Detection and immunolabeling of peroxisomal proteins. Peroxisomes: Methods and Protocols, 113-130.
Mouse Anti-ACAD11 Recombinant Antibody (B-12) (CBMAB-A0426-YC) in WB
Mitochondrial protein extracts were analyzed by SDS/PAGE and immunoblot (IB) with indicated antibodies. WT mice fed ad libitum. ...View More
Neufeld-Cohen, A., Robles, M. S., Aviram, R., Manella, G., Adamovich, Y., Ladeuix, B., ... & Asher, G. (2016). Circadian control of oscillations in mitochondrial rate-limiting enzymes and nutrient utilization by PERIOD proteins. Proceedings of the National Academy of Sciences, 113(12), E1673-E1682.

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
V2-179169
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Specific for an epitope mapping between amino acids 120-140 within an internal region of ACAD-11 of human origin
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Acyl-CoA Dehydrogenase Family Member 11
Introduction
ACAD11 is an acyl-CoA dehydrogenase enzyme with a preference for carbon chain lengths between 20 and 26. Naturally occurring read-through transcription occurs between the upstream gene NPHP3 (nephronophthisis 3 (adolescent)) and this gene.
Entrez Gene ID
Human84129
Mouse102632
Rat315973
UniProt ID
HumanQ709F0
MouseQ80XL6
RatB3DMA2
Alternative Names
Acyl-CoA Dehydrogenase Family Member 11; Acyl-Coenzyme A Dehydrogenase Family, Member 11; ACAD-11; Acyl-CoA Dehydrogenase Family, Member 11; EC 1.3.99.-;
Function
Acyl-CoA dehydrogenase, that exhibits maximal activity towards saturated C22-CoA (PubMed:21237683). Probably participates in beta-oxydation and energy production but could also play a role in the metabolism of specific fatty acids to control fatty acids composition of cellular lipids in brain (Probable).
Biological Process
Fatty acid beta-oxidation
Fatty acid beta-oxidation using acyl-CoA dehydrogenase
Cellular Location
Peroxisome; Mitochondrion membrane. Has been detected associated with mitochondrial membrane, but no matrix, in kidney and cerebellum, as well as in a neuroblastoma cell line, but not in skin fibroblasts, where it is observed in cytoplasmic vesicles (PubMed:21237683). No mitochondrial targeting signals could be predicted for any known isoform, including a putative isoform starting at Met-316.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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