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Rabbit Anti-Acetyl-H2AFZ (Lys4/Lys7) Recombinant Antibody (V2-621480) (CBMAB-CP0897-LY)

The product is antibody recognizes Acetyl-H2AFZ (Lys4/Lys7). The antibody D3V1I immunoassay techniques such as: WB,IP,FC,CIP.
See all Acetyl-H2AZ1 (Lys4/Lys7) antibodies
Published Data
Rabbit Anti-Acetyl-H2AFZ (Lys4/Lys7) Recombinant Antibody (D3V1I) (CBMAB-CP0897-LY) in WB
Western blot validation of changes in histone acetylation in Hdac1 KO, SAHA and MS-275 samples. ...View More
Zrimšek, M., Kuchaříková, H., Draganić, K., Dobrovolná, P., Heiss Spornberger, V., Winkelmayer, L., ... & Egger, G. (2022). Quantitative Acetylomics Uncover Acetylation-Mediated Pathway Changes Following Histone Deacetylase Inhibition in Anaplastic Large Cell Lymphoma. Cells, 11(15), 2380.

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat
Clone
V2-621480
Antibody Isotype
IgG
Application
WB, IP, IHC-F, CI

Basic Information

Immunogen
Synthetic peptide corresponding to residues surrounding acetylated Lys4 and Lys7 of human H2AZ protein.
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000
IP1:200
FC1:50
CIP1:50

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
HEPES pH 7.5, 0.01% BSA, 50% Glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Introduction
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality.
Entrez Gene ID
Human3015
Mouse51788
Rat58940
UniProt ID
HumanP0C0S5
MouseP0C0S6
RatP0C0S7
Alternative Names
H2AZ; H2A.z; H2A/z; H2A.Z-1
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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