What specific region of ACHE does this antibody recognize?

This antibody was generated against a specific recombinant fragment of the human ACHE protein, ensuring high specificity. This targeted approach minimizes cross-reactivity with other esterases.

Does this antibody detect all isoforms of ACHE?

This antibody is designed to recognize the primary synaptic ("T" or "tailed") isoform of ACHE, which is the most common form in the brain and at the neuromuscular junction. Its reactivity with other, rarer isoforms (e.g., "R" or "H" isoforms) would need to be specifically tested.

Mouse Anti-ACHE Recombinant Antibody (V2-179295) (CBMAB-A0571-YC)

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Datasheet

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Basic Information

Host Animal

Mouse

Clone

V2-179295

Application

ELISA, ICC, IHC, WB

Immunogen

Recombinant AchE (Phe377-Thr574) expressed in E. coli

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:400
IHC-P	1:50-1:200
IHC-F	1:100-1:500
IF(ICC)	1:100-1:500
ELISA	1:100-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol

Buffer

PBS, pH7.2, 50% Glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Acetylcholinesterase (Cartwright Blood Group)

Introduction

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood gro

Entrez Gene ID

UniProt ID

P22303

Alternative Names

Acetylcholinesterase (Cartwright Blood Group); Acetylcholinesterase (YT Blood Group); Yt Blood Group; EC 3.1.1.7; Apoptosis-Related Acetylcholinesterase; Acetylcholinesterase; EC 3.1.1;

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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