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Mouse Anti-ACHE Recombinant Antibody (V2-6063) (CBMAB-0008CQ)

This product is a mouse antibody that recognizes ACHE. The antibody HR2 can be used for immunoassay techniques such as: ELISA, FC, IF, IHC-Fr, IP.
See all ACHE antibodies
Published Data
Fig.1 Acetylcholinesterase (AChE) colocalizes with presenilin-1 (PS1) within lipid rafts. Monoclonal Anti-ACHE antibody (Cat# CBMAB-0008CQ, Clone HR2) is used for immunofluorescence test, (A) blue for ACHE; (B) for ACHE.
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Fig.2 AChE immunoreactivity in amyloid plaques. Monoclonal Anti-ACHE antibody (Cat# CBMAB-0008CQ, Clone HR2) is used.
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Fig.3 CSF±AChE levels, taken before and after treatment, in a dose-dependant manner. Monoclonal Anti-ACHE antibody (Cat# CBMAB-0008CQ, Clone HR2) is used to perform this ELISA assay.
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Fig.1 Human embryonic brain extracts were immunoprecipitated with or anti-AChE MA3-042 antibodies.
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Fig.2 Cellular colocalization of PS1 and AChE.
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Fig.3 Immunohistochemical detection of PS1 and AChE in human frontal cortex.
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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rabbit, Guinea pig, Cattle, Cat, Monkey
Clone
V2-6063
Antibody Isotype
IgG2b
Application
ELISA, FC, IF, IHC-Fr, IP

Basic Information

Immunogen
Full length protein of Human Acetylcholinesterase
Specificity
Human, Mouse, Rabbit, Guinea pig, Cattle, Cat, Monkey
Antibody Isotype
IgG2b
Clonality
Monoclonal
Application Notes
This HR2 antibody has been successfully used in applications of IF, IHC, IP and ELISA.
For IF, the recommended dilution is 1:100-1:1000.
For IHC, the recommended dilution is 1:50.
For flow cytometry, the ideal concentration is 1µg for 10^6 cells.
For IP and ELISA, please use at an specific assay dependent concentration.
It's not applicable for western blot. It's recommended that the optimal antibody concentration, dilution, incubation time etc. are to be determined by the end user.
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:100-1:2,000
IF(ICC)1:100-1:1,000
IHC1:10-1:500
IHC-P1:10-1:500
IHC-F1:10-1:50
IP1:10-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS
Preservative
0.05% sodium azide
Concentration
1 mg/ml
Purity
>95% as determined by analysis by SDS-PAGE
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Acetylcholinesterase (Cartwright Blood Group)
Introduction
Acetylcholinesterase (ACHE; EC 3.1.1.7) controls synaptic and neurohumoral cholinergic activity by hydrolyzing the neurotransmitter acetylcholine. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. ACHE function relies on precise regulation of its expression and localization. In particular, alternative splicing of the 3-prime region of ACHE results in ACHE isoforms with distinct C-terminal peptides that determine posttranslational maturation and oligomeric assembly. Acetylcholinesterase is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen.
Entrez Gene ID
Human43
Mouse11423
Guinea pig100422792
Cattle540446
Cat493674
Monkey713370
UniProt ID
HumanP22303
MouseP21836
RabbitQ29499
Guinea pigD3YE96
CattleP23795
CatO62763
MonkeyD4P3V7
Alternative Names
YT; ACEE; ARACHE; N-ACHE
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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