Mouse Anti-ACKR3 Recombinant Antibody (CBT4137) (V2LY-0625-LY1906)

Name: Mouse Anti-ACKR3 Recombinant Antibody (CBT4137)
SKU: V2LY-0625-LY1906
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-ACKR3 Recombinant Antibody (CBT4137) (V2LY-0625-LY1906) in ELISA

Mouse Anti-ACKR3 Recombinant Antibody (CBT4137) (V2LY-0625-LY1906) in WB

Mouse Anti-ACKR3 Recombinant Antibody (CBT4137) (V2LY-0625-LY1906) in IF

Mouse Anti-ACKR3 Recombinant Antibody (CBT4137) (V2LY-0625-LY1906) in FC

Mouse Anti-ACKR3 Recombinant Antibody (CBT4137) (V2LY-0625-LY1906) in IHC

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT4137

Application

IHC, ICC, FC

Immunogen

Purified recombinant fragment of human CXCR7(AA: extra mix) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
ICC	1:50-1:200
FC	1:200-1:400
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Atypical Chemokine Receptor 3

Entrez Gene ID

57007

UniProt ID

P25106

Function

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CXCL11 and CXCL12/SDF1. Chemokine binding leads to ligand internalization and activation of MAPK signaling pathway. Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness. In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival. Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration. Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12. Required for heart valve development. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates.

Biological Process

Angiogenesis
Calcium-mediated signaling
Cell adhesion
Cell chemotaxis
Chemokine-mediated signaling pathway
G protein-coupled receptor signaling pathway
Immune response
Negative regulation of cell population proliferation
Negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage
Positive regulation of cytosolic calcium ion concentration
Positive regulation of ERK1 and ERK2 cascade
Positive regulation of mesenchymal stem cell migration
Receptor internalization
Vasculogenesis
Viral process

Topology

Extracellular: 1-40 aa
Helical: 41-61 aa
Cytoplasmic: 62-81 aa
Helical: 82-102 aa
Extracellular: 103-118 aa
Helical: 119-139 aa
Cytoplasmic: 140-162 aa
Helical: 163-183 aa
Extracellular: 184-213 aa
Helical: 214-234 aa
Cytoplasmic: 235-252 aa
Helical: 253-273 aa
Extracellular: 274-296 aa
Helical: 297-319 aa
Cytoplasmic: 320-362 aa

PTM

The Ser/Thr residues in the C-terminal cytoplasmic tail may be phosphorylated.
Ubiquitinated at the Lys residues in its C-terminal cytoplasmic tail and is essential for correct trafficking from and to the cell membrane. Deubiquitinated by CXCL12-stimulation in a reversible manner.

Meyrath, M., Szpakowska, M., Zeiner, J., Massotte, L., Merz, M. P., Benkel, T., ... & Chevigné, A. (2020). The atypical chemokine receptor ACKR3/CXCR7 is a broad-spectrum scavenger for opioid peptides. Nature communications, 11(1), 1-16.

Pan, L., Lv, J., Zhang, Z., & Zhang, Y. (2018). Adaptation and Constraint in the Atypical Chemokine Receptor Family in Mammals. BioMed research international, 2018.

Silwedel, C., Speer, C. P., Haarmann, A., Fehrholz, M., Claus, H., Buttmann, M., & Glaser, K. (2018). Novel insights into neuroinflammation: bacterial lipopolysaccharide, tumor necrosis factor α, and Ureaplasma species differentially modulate atypical chemokine receptor 3 responses in human brain microvascular endothelial cells. Journal of neuroinflammation, 15(1), 1-11.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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