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Mouse Anti-ACOT8 Recombinant Antibody (V2-179351) (CBMAB-A0652-YC)

Provided herein is a Mouse monoclonal antibody against Human Acyl-CoA Thioesterase 8. The antibody can be used for immunoassay techniques, such as ELISA, FC, ICC, IF, WB.
See all ACOT8 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
V2-179351
Antibody Isotype
IgG
Application
ELISA, FC, ICC, IF, WB

Basic Information

Immunogen
Recombinant protein corresponding to human ACOT8, aa1-319, expressed in E. coli
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol
Buffer
PBS, pH7.4, 10% glycerol
Preservative
0.02% sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Acyl-CoA Thioesterase 8
Introduction
ACOT8 is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of
Entrez Gene ID
10005
UniProt ID
O14734
Alternative Names
Acyl-CoA Thioesterase 8; Nef (Lentivirus Myristoylated Factor) Associated Protein 1; Peroxisomal Acyl-Coenzyme A Thioester Hydrolase 1; Peroxisomal Long-Chain Acyl-CoA Thioesterase 1; Peroxisomal Acyl-CoA Thioesterase 1; Choloyl-Coenzyme A Thioesterase; C
Function
Acyl-coenzyme A (acyl-CoA) thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Acyl-coenzyme A thioesterase 8/ACOT8 display no strong substrate specificity with respect to the carboxylic acid moiety of Acyl-CoAs (By similarity). Hydrolyzes medium length (C2 to C20) straight-chain, saturated and unsaturated acyl-CoAS but is inactive towards substrates with longer aliphatic chains. Moreover, it catalyzes the hydrolysis of CoA esters of bile acids, such as choloyl-CoA and chenodeoxycholoyl-CoA and competes with bile acid CoA:amino acid N-acyltransferase (BAAT) (By similarity). ACOT8 is also able to hydrolyze CoA esters of dicarboxylic acids (By similarity). It is involved in the metabolic regulation of peroxisome proliferation.
(Microbial infection) May mediate Nef-induced down-regulation of CD4 cell-surface expression.
Biological Process
Acyl-CoA metabolic process
Alpha-linolenic acid metabolic process
Bile acid biosynthetic process
Dicarboxylic acid catabolic process
Fatty acid beta-oxidation using acyl-CoA oxidase
Fatty acid catabolic process
Negative regulation of CD4 production
Peroxisome fission
Protein localization
Viral process
Cellular Location
Peroxisome matrix. Predominantly localized in the peroxisome but a localization to the cytosol cannot be excluded.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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