Mouse Anti-ACOT8 Recombinant Antibody (V2-179351) (CBMAB-A0652-YC)

Name: Mouse Anti-ACOT8 Recombinant Antibody (V2-179351)
SKU: CBMAB-A0652-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

V2-179351

Application

ELISA, FC, ICC, IF, WB

Immunogen

Recombinant protein corresponding to human ACOT8, aa1-319, expressed in E. coli

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid in PBS, pH 7.4, 0.02% sodium azide, 10% glycerol

Buffer

PBS, pH7.4, 10% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Acyl-CoA Thioesterase 8

Introduction

ACOT8 is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of

Entrez Gene ID

10005

UniProt ID

O14734

Alternative Names

Acyl-CoA Thioesterase 8; Nef (Lentivirus Myristoylated Factor) Associated Protein 1; Peroxisomal Acyl-Coenzyme A Thioester Hydrolase 1; Peroxisomal Long-Chain Acyl-CoA Thioesterase 1; Peroxisomal Acyl-CoA Thioesterase 1; Choloyl-Coenzyme A Thioesterase; C

Function

Acyl-coenzyme A (acyl-CoA) thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Acyl-coenzyme A thioesterase 8/ACOT8 display no strong substrate specificity with respect to the carboxylic acid moiety of Acyl-CoAs (By similarity). Hydrolyzes medium length (C2 to C20) straight-chain, saturated and unsaturated acyl-CoAS but is inactive towards substrates with longer aliphatic chains. Moreover, it catalyzes the hydrolysis of CoA esters of bile acids, such as choloyl-CoA and chenodeoxycholoyl-CoA and competes with bile acid CoA:amino acid N-acyltransferase (BAAT) (By similarity). ACOT8 is also able to hydrolyze CoA esters of dicarboxylic acids (By similarity). It is involved in the metabolic regulation of peroxisome proliferation.
(Microbial infection) May mediate Nef-induced down-regulation of CD4 cell-surface expression.

Biological Process

Acyl-CoA metabolic process
Alpha-linolenic acid metabolic process
Bile acid biosynthetic process
Dicarboxylic acid catabolic process
Fatty acid beta-oxidation using acyl-CoA oxidase
Fatty acid catabolic process
Negative regulation of CD4 production
Peroxisome fission
Protein localization
Viral process

Cellular Location

Peroxisome matrix. Predominantly localized in the peroxisome but a localization to the cytosol cannot be excluded.

Cholico, G. N., Fling, R. R., Zacharewski, N. A., Fader, K. A., Nault, R., & Zacharewski, T. R. (2021). Thioesterase induction by 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin results in a futile cycle that inhibits hepatic β-oxidation. bioRxiv.

Xu, C. L., Chen, L., Li, D., Chen, F. T., Sha, M. L., & Shao, Y. (2020). Acyl-CoA Thioesterase 8 and 11 as Novel Biomarkers for Clear Cell Renal Cell Carcinoma. Frontiers in genetics, 11, 1576.

Palmeira, J. D. F., Argañaraz, G. A., de Oliveira, G. X. L. M., & Argañaraz, E. R. (2019). Physiological relevance of ACOT8‐Nef interaction in HIV infection. Reviews in medical virology, 29(5), e2057.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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