Mouse Anti-ACOX2 Recombinant Antibody (V2-179363) (CBMAB-A0664-YC)

Name: Mouse Anti-ACOX2 Recombinant Antibody (V2-179363)
SKU: CBMAB-A0664-YC
Rating: 5 (1 reviews)

See all ACOX2 antibodies

Online Inquiry

Published Data

Mouse Anti-ACOX2 Recombinant Antibody (A-7) (CBMAB-A0664-YC) in WB

Western blot showing decreased protein expression of CPT1 and other fatty acid oxidation enzymes, acyl-CoA oxidase 2 (Acox2), in CLP kidneys normalized to β-actin. ...View More

Li, Y., Nourbakhsh, N., Pham, H., Tham, R., Zuckerman, J. E., & Singh, P. (2020). Evolution of altered tubular metabolism and mitochondrial function in sepsis-associated acute kidney injury. American Journal of Physiology-Renal Physiology, 319(2), F229-F244.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-179363

Antibody Isotype

IgG1, κ

Application

WB, IP, IF, ELISA

Basic Information

Immunogen

Specific for an epitope mapping between amino acids 651-672 near the C-terminus of ACOX2 of human origin

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer

PBS, 0.1% gelatin

Preservative

< 0.1% sodium azide

Concentration

0.2 mg/ml

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

acyl-Coenzyme A oxidase 2, branched chain

Introduction

ACOX2 belongs to the acyl-CoA oxidase family. It encodes the branched-chain acyl-CoA oxidase which is involved in the degradation of long branched fatty acids and bile acid intermediates in peroxisomes. Deficiency of this enzyme results in the accumulatio

Entrez Gene ID

8309

UniProt ID

Q99424

Alternative Names

Acyl-CoA Oxidase 2; 3-Alpha,7-Alpha,12-Alpha-Trihydroxy-5-Beta-Cholestanoyl-CoA 24-Hydroxylase; Trihydroxycoprostanoyl-CoA Oxidase; 3-Alpha,7-Alpha,12-Alpha-Trihydroxy-5-Beta-Cholestanoyl-CoA Oxidase; Acyl-Coenzyme A Oxidase 2, Branched Chain; Acyl-CoA Ox

Function

Oxidizes the CoA esters of the bile acid intermediates di- and tri-hydroxycholestanoic acids. Capable of oxidizing short as well as long chain 2-methyl branched fatty acids (By similarity).

Biological Process

Bile acid biosynthetic process
Fatty acid beta-oxidation using acyl-CoA oxidase
Lipid homeostasis
Positive regulation of cell death
Positive regulation of response to oxidative stress
Protein localization
Very long-chain fatty acid metabolic process

Cellular Location

Peroxisome

Involvement in disease

Congenital bile acid synthesis defect 6 (CBAS6): An inborn error of bile acid synthesis characterized by abnormally increased liver enzymes, hypolipidemia and low cholesterol, vitamin D deficiency, elevated plasma and urinary levels of C27 intermediate bile acids 3alpha,7alpha-dihydroxy-5beta-cholestanoic acid (DHCA) and 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoic acid (THCA). Serum levels of phytanic and pristanic acids are normal. Clinical features include liver fibrosis, mild ataxia, delayed development, and cognitive impairment. Liver histology shows many thin fibrous septa, swollen hepatocytes, glycogenated nuclei, and focal acinar transformation, consistent with hepatocellular injury and regeneration, without signs of obvious cholestasis, cholate stasis, or steatosis. CBAS6 transmission pattern is consistent with autosomal recessive inheritance.

More Infomation

References

Okamura, M., Ueno, T., Tanaka, S., Murata, Y., Kobayashi, H., Miyamoto, A., ... & Fukuda, N. (2021). Increased expression of acyl-CoA oxidase 2 in the kidney with plasma phytanic acid and altered gut microbiota in spontaneously hypertensive rats. Hypertension Research, 44(6), 651-661.

Zhang, Q., Zhang, Y., Sun, S., Wang, K., Qian, J., Cui, Z., ... & Zhou, J. (2021). ACOX2 is a prognostic marker and impedes the progression of hepatocellular carcinoma via PPARα pathway. Cell Death & Disease, 12(1), 1-12.

Alonso-Pena, M., HERMANS, H., Herraez, E., Briz, O., MONTE, M., Sanchez-Martin, M., ... & MARIN, J. (2019, April). Genetic and pathophysiological factors leading to deficient acyl-CoA oxidase 2 (ACOX2) activity in hepatocytes, an alteration which causes oxidative and endoplasmic reticulum stress in liver cells. In Journal of Hepatology (Vol. 70, pp. E579-E579). PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS: ELSEVIER SCIENCE BV.

Ferdinandusse, S., Denis, S., van Roermund, C. W., Preece, M. A., Koster, J., Ebberink, M. S., ... & Wanders, R. J. (2018). A novel case of ACOX2 deficiency leads to recognition of a third human peroxisomal acyl-CoA oxidase. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1864(3), 952-958.

Zhou, X., & Wang, H. (2017). ACOX2 deficiency in primary malignant cardiac tumors. Proceedings of the National Academy of Sciences, 114(18), E3590-E3591.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-ACOX2 Recombinant Antibody (V2-179363)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Associated Products

Related Products

Mouse Anti-ACOX2 Recombinant Antibody (V2-179361)

Mouse Anti-ACOX2 Recombinant Antibody (V2-179362)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: