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Mouse Anti-ACR Recombinant Antibody (V2-179398) (CBMAB-A0700-YC)

Provided herein is a Mouse monoclonal antibody against Pig Acrosin. The antibody can be used for immunoassay techniques, such as WB, FC, ICC, IF.
See all ACR antibodies

Summary

Host Animal
Mouse
Specificity
Pig
Clone
V2-179398
Antibody Isotype
IgG1
Application
WB, FC, ICC, IF

Basic Information

Immunogen
Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization
Specificity
Pig
Antibody Isotype
IgG1
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
FC1-12 µg/ml
IF(ICC)1:10-1:2,000
WB1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, pH7.4
Preservative
15mM sodium azide
Concentration
1 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
acrosin precursor (EC 3.4.21.10)
Introduction
Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the
Entrez Gene ID
397098
UniProt ID
P08001
Alternative Names
Acrosin; Acrosin Light And Heavy Chain Prepropeptide; Preproacrosin; EC 3.4.21.10; Proacrosin; EC 3.4.21; ACRS;
Function
Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.
Biological Process
Acrosome reaction
Activation of adenylate cyclase activity
Proteolysis
Single fertilization
Cellular Location
Acrosomal matrix
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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