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  • Mouse Anti-ACRV1 Recombinant Antibody (V2-179430)

Mouse Anti-ACRV1 Recombinant Antibody (V2-179430) (CBMAB-A0736-YC)

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Published Data
Mouse Anti-ACRV1 Recombinant Antibody (A-9) (CBMAB-A0736-YC) in WB
Mouse Anti-ACRV1 Recombinant Antibody (A-9) (CBMAB-A0736-YC) in WB
Western blot analysis of Acrv1 protein expression in mouse testis, with β-actin used as the internal control. ...View More
Fang, X., Gamallat, Y., Chen, Z., Mai, H., Zhou, P., Sun, C., ... & Li, N. (2021). Hypomorphic and hypermorphic mouse models of Fsip2 indicate its dosage-dependent roles in sperm tail and acrosome formation. Development, 148(11), dev199216.
Mouse Anti-ACRV1 Recombinant Antibody (A-9) (CBMAB-A0736-YC) in IF
Mouse Anti-ACRV1 Recombinant Antibody (A-9) (CBMAB-A0736-YC) in IF
Immunofluorescence staining of cauda spermatozoa of WT mice showing the co-expression of Acrv1 (red). The dashed orange lines indicate the magnified area below. ...View More
Fang, X., Gamallat, Y., Chen, Z., Mai, H., Zhou, P., Sun, C., ... & Li, N. (2021). Hypomorphic and hypermorphic mouse models of Fsip2 indicate its dosage-dependent roles in sperm tail and acrosome formation. Development, 148(11), dev199216.
Mouse Anti-ACRV1 Recombinant Antibody (A-9) (CBMAB-A0736-YC) in IHC
Mouse Anti-ACRV1 Recombinant Antibody (A-9) (CBMAB-A0736-YC) in IHC
Immunostaining of Acrv1 (red) in seminiferous tubules of testicular sections at stage VII-VIII. Top images show tissue immunostaining of WT. ...View More
Fang, X., Gamallat, Y., Chen, Z., Mai, H., Zhou, P., Sun, C., ... & Li, N. (2021). Hypomorphic and hypermorphic mouse models of Fsip2 indicate its dosage-dependent roles in sperm tail and acrosome formation. Development, 148(11), dev199216.
Datasheet
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Basic Information

Host Animal
Mouse
Clone
V2-179430
Application
WB, IP, IF, ELISA
Immunogen
Raised against amino acids 138-261 mapping at the C-terminus of ACRV1 of mouse origin
Specificity
Mouse
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation

Target

Full Name
Acrosomal Vesicle Protein 1
Introduction
ACRV1 is a testis-specific, differentiation antigen, acrosomal vesicle protein 1, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. The acrosomal vesicle protein 1 m
Entrez Gene ID
11451
UniProt ID
P50289
Alternative Names
Acrosomal Vesicle Protein 1; Sperm Protein 10; Acrosomal Protein SP-10; D11S4365; SPACA2; SP-10;
Biological Process
Multicellular organism development
Cellular Location
Acrosome. Nascent acrosomal vesicle of Golgi phase spermatids.

Cruz, A., Sullivan, D. B., Doty, K. F., Hess, R. A., Canisso, I. F., & Reddi, P. P. (2020). Acrosomal marker SP-10 (gene name Acrv1) for staging of the cycle of seminiferous epithelium in the stallion. Theriogenology, 156, 214-221.

Urekar, C., Acharya, K. K., Chhabra, P., & Reddi, P. P. (2019). A 50-bp enhancer of the mouse acrosomal vesicle protein 1 gene activates round spermatid-specific transcription in vivo. Biology of reproduction, 101(4), 842-853.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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