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Mouse Anti-ACRV1 Recombinant Antibody (V2-179430) (CBMAB-A0736-YC)


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Published Data
Mouse Anti-ACRV1 Recombinant Antibody (A-9) (CBMAB-A0736-YC) in WB
Western blot analysis of Acrv1 protein expression in mouse testis, with β-actin used as the internal control. ...View More
Fang, X., Gamallat, Y., Chen, Z., Mai, H., Zhou, P., Sun, C., ... & Li, N. (2021). Hypomorphic and hypermorphic mouse models of Fsip2 indicate its dosage-dependent roles in sperm tail and acrosome formation. Development, 148(11), dev199216.
Mouse Anti-ACRV1 Recombinant Antibody (A-9) (CBMAB-A0736-YC) in IF
Immunofluorescence staining of cauda spermatozoa of WT mice showing the co-expression of Acrv1 (red). The dashed orange lines indicate the magnified area below. ...View More
Fang, X., Gamallat, Y., Chen, Z., Mai, H., Zhou, P., Sun, C., ... & Li, N. (2021). Hypomorphic and hypermorphic mouse models of Fsip2 indicate its dosage-dependent roles in sperm tail and acrosome formation. Development, 148(11), dev199216.
Mouse Anti-ACRV1 Recombinant Antibody (A-9) (CBMAB-A0736-YC) in IHC
Immunostaining of Acrv1 (red) in seminiferous tubules of testicular sections at stage VII-VIII. Top images show tissue immunostaining of WT. ...View More
Fang, X., Gamallat, Y., Chen, Z., Mai, H., Zhou, P., Sun, C., ... & Li, N. (2021). Hypomorphic and hypermorphic mouse models of Fsip2 indicate its dosage-dependent roles in sperm tail and acrosome formation. Development, 148(11), dev199216.

Summary

Host Animal
Mouse
Specificity
Mouse
Clone
V2-179430
Antibody Isotype
IgG2a, κ
Application
WB, IP, IF, ELISA

Basic Information

Immunogen
Raised against amino acids 138-261 mapping at the C-terminus of ACRV1 of mouse origin
Specificity
Mouse
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500
ELISA1:30-1:3,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Buffer
PBS, 0.1% gelatin
Preservative
< 0.1% sodium azide
Concentration
0.2 mg/ml
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Acrosomal Vesicle Protein 1
Introduction
ACRV1 is a testis-specific, differentiation antigen, acrosomal vesicle protein 1, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. The acrosomal vesicle protein 1 m
Entrez Gene ID
11451
UniProt ID
P50289
Alternative Names
Acrosomal Vesicle Protein 1; Sperm Protein 10; Acrosomal Protein SP-10; D11S4365; SPACA2; SP-10;
Biological Process
Multicellular organism development
Cellular Location
Acrosome. Nascent acrosomal vesicle of Golgi phase spermatids.
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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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