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Mouse Anti-ACTA1 Recombinant Antibody (V2-179480) (CBMAB-A0796-YC)

Provided herein is a Mouse monoclonal antibody against Human Actin, Alpha 1, Skeletal Muscle. The antibody can be used for immunoassay techniques, such as IHC-P, IHC-Fr, DID, ICC, IF.
See all ACTA1 antibodies
Published Data
Fig.1 Determination of anti-actin immunoreactivity by Western immunoblotting.
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Fig.2 NAi-mediated knockdown of MUC4 expression increased Herceptin binding.
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Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Guinea pig, Hamster, Cattle, Dog, Pig, Frog
Clone
V2-179480
Antibody Isotype
IgG2a
Application
IHC-P, IHC-Fr, DID, ICC, IF

Basic Information

Immunogen
Synthetic peptide: SGPSIVHALCP (human) attached to Multiple Antigen Peptide (MAP) backbone.
Specificity
Human, Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Guinea pig, Hamster, Cattle, Dog, Pig, Frog
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:100-1:2,000
WB1:500
IHC1:10-1:500
IF(ICC)1:200
IHC-F1:10-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Ascites
Preservative
15mM sodium azide
Concentration
Batch dependent
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Actin, Alpha 1, Skeletal Muscle
Introduction
ACTA1 belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the co
Entrez Gene ID
Human58
Dog488984
Rat29437
Cattle281592
Pig100154254
Mouse11459
Sheep101107662
Rabbit100009506
Goat102182476
Chicken421534
GuineaPig
Hamster100768972
Frog448346
UniProt ID
HumanP68133
DogF1PVC1
RatP68136
CattleP68138
PigP68137
MouseP68134
SheepW5NYJ1
RabbitP68135
GoatA0A452EFE6
ChickenP68139
GuineaPig
HamsterA0A1U7QEW4
FrogP04751
Alternative Names
Actin, Alpha 1, Skeletal Muscle; Nemaline Myopathy Type 3; ACTA; Actin, Alpha Skeletal Muscle; Alpha-Actin-1; CFTD1; CFTDM; ASMA;
Function
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Biological Process
Cellular response to organonitrogen compound
Mesenchyme migration
Muscle contraction
Muscle filament sliding
Positive regulation of gene expression
Response to extracellular stimulus
Response to lithium ion
Response to mechanical stimulus
Response to steroid hormone
Skeletal muscle fiber adaptation
Skeletal muscle fiber development
Skeletal muscle thin filament assembly
Cellular Location
Cytoskeleton
Involvement in disease
Nemaline myopathy 3 (NEM3): A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-like or rod-shaped structures in muscle fibers on histologic examination.
Myopathy, actin, congenital, with excess of thin myofilaments (MPCETM): A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Myopathy, congenital, with fiber-type disproportion (CFTD): A genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions.
Myopathy, scapulohumeroperoneal (SHPM): An autosomal dominant muscular disorder characterized by progressive muscle weakness with initial scapulo-humeral-peroneal and distal distribution. Over time, muscle weakness progresses to proximal muscle groups. Clinical characteristics include scapular winging, mild lower facial weakness, foot drop due to foot eversion and dorsiflexion weakness, and selective muscle atrophy. Age at onset and disease progression are variable.
PTM
Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization.
Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
Methylated at His-75 by SETD3.
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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