Mouse Anti-ACTG1 Recombinant Antibody (V2-179591) (CBMAB-A0909-YC)

Provided herein is a Mouse monoclonal antibody against Human Actin Gamma 1. The antibody can be used for immunoassay techniques, such as ELISA, FC, IF, IHC, WB.
See all ACTG1 antibodies

Published Data

Fig.1 Western blotting was performed using clone B4 to determine level of all muscle actin isoforms.

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

V2-179591

Antibody Isotype

IgG2b

Application

ELISA, FC, IF, IHC, WB

Basic Information

Immunogen

Synthetic peptide corresponding to the N-terminal nonapeptide of gamma-cytoplasmic actin, coupled to KLH

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid in PBS, 0.09% sodium azide

Buffer

PBS

Preservative

0.09% sodium azide

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name

actin, gamma 1

Introduction

Actins are highly conserved proteins that are involved in various types of cell motility and in maintenance of the cytoskeleton. Three main groups of actin isoforms have been identified in vertebrate animals: alpha, beta, and gamma. The alpha actins are f

Entrez Gene ID

UniProt ID

P63261

Alternative Names

ACT; ACTG; DFNA20; DFNA26; HEL-176

Function

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Biological Process

Angiogenesis
Cell junction assembly
Ephrin receptor signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
Maintenance of blood-brain barrier
Membrane organization
Morphogenesis of a polarized epithelium
Platelet aggregation
Positive regulation of cell migration
Positive regulation of gene expression
Positive regulation of wound healing
Protein localization to bicellular tight junction
Regulation of focal adhesion assembly
Regulation of stress fiber assembly
Regulation of transepithelial transport
Retina homeostasis
Tight junction assembly

Cellular Location

Cytoskeleton

Involvement in disease

Deafness, autosomal dominant, 20 (DFNA20): A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Baraitser-Winter syndrome 2 (BRWS2): A rare developmental disorder characterized by the combination of congenital ptosis, high-arched eyebrows, hypertelorism, ocular colobomata, and a brain malformation consisting of anterior-predominant lissencephaly. Other typical features include postnatal short stature and microcephaly, intellectual disability, seizures, and hearing loss.

PTM

Oxidation of Met-44 and Met-47 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization.
Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
N-terminal acetylation by NAA80 affects actin filament depolymerization and elongation, including elongation driven by formins. In contrast, filament nucleation by the Arp2/3 complex is not affected.
Methylated at His-73 by SETD3.
(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.

References

Richter, C., Mayhew, D., Rennhack, J. P., So, J., Stover, E. H., Hwang, J. H., & Szczesna-Cordary, D. (2020). Genomic amplification and functional dependency of the gamma actin gene actg1 in uterine cancer. International journal of molecular sciences, 21(22), 8690.

Chen, S., Wang, X., Yuan, J., Deng, C., Xie, X., & Kang, J. (2020). Reduced levels of actin gamma 1 predict poor prognosis in ovarian cancer patients. Journal of Obstetrics and Gynaecology Research, 46(9), 1827-1834.

Malek, N., Mrówczyńska, E., Michrowska, A., Mazurkiewicz, E., Pavlyk, I., & Mazur, A. J. (2020). Knockout of ACTB and ACTG1 with CRISPR/Cas9 (D10A) technique shows that non-muscle β and γ actin are not equal in relation to human melanoma cells’ motility and focal adhesion formation. International journal of molecular sciences, 21(8), 2746.

Liu, J., Liu, H., Zhao, Z., Wang, J., Guo, D., & Liu, Y. (2020). Regulation of Actg1 and Gsta2 is possible mechanism by which capsaicin alleviates apoptosis in cell model of 6-OHDA-induced Parkinson's disease. Bioscience Reports, 40(6), BSR20191796.

Yan, Y., Xu, H., Zhang, L., Zhou, X., Qian, X., Zhou, J., ... & Wang, W. (2019). RRAD suppresses the Warburg effect by downregulating ACTG1 in hepatocellular carcinoma. OncoTargets and therapy, 12, 1691.

Dong, X., Han, Y., Sun, Z., & Xu, J. (2018). Actin Gamma 1, a new skin cancer pathogenic gene, identified by the biological feature‐based classification. Journal of cellular biochemistry, 119(2), 1406-1419.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

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Mouse Anti-ACTG1 Recombinant Antibody (V2-179596)

Mouse Anti-ACTG1 Recombinant Antibody (V2-179597)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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