Why is a recombinant antibody preferable to a traditional monoclonal antibody for detecting active Caspase-3?

Recombinant antibodies are produced using genetic engineering, which ensures exceptional batch-to-batch consistency and purity. For a difficult target like an active/cleaved protein, a recombinant antibody offers superior specificity and minimal background. This is crucial for distinguishing the cleaved form from its inactive precursor, ensuring more reliable and reproducible data.

Can this antibody be used in conjunction with other antibodies for multiplex analysis?

Yes, this is a mouse monoclonal antibody, so it is compatible with standard multiplexing protocols. To avoid cross-reactivity, you should use a primary antibody from a different host species (e.g., rabbit or goat) for your other target and use species-specific secondary antibodies (e.g., anti-mouse for this antibody and anti-rabbit for the other) that are conjugated with different fluorophores or enzymes.

Mouse Anti-Active CASP3 Recombinant Antibody (EC7) (V2LY-0624-LY7)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

EC7

Application

WB, IHC-P, IF

Immunogen

Recombinant Protein.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:1,000
IHC-P	1:100-1:200
IF(CC)	1:100-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, pH 7.4, 50% glycerol

Preservative

0.02% sodium azide

Concentration

Batch dependent

Purity

>95% by SDS Page

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Caspase 3

Introduction

The protein encoded by this gene is a cysteine-aspartic acid protease that plays a central role in the execution-phase of cell apoptosis. The encoded protein cleaves and inactivates poly(ADP-ribose) polymerase while it cleaves and activates sterol regulatory element binding proteins as well as caspases 6, 7, and 9. This protein itself is processed by caspases 8, 9, and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease.

Entrez Gene ID

Human	836
Mouse	12367
Rat	25402

UniProt ID

Human	P42574
Mouse	P70677
Rat	P55213

Alternative Names

Caspase 3; Caspase 3, Apoptosis-Related Cysteine Peptidase; Caspase 3, Apoptosis-Related Cysteine Protease; SREBP Cleavage Activity 1; Cysteine Protease CPP32; Protein Yama; EC 3.4.22.56; Apopain; CASP-3;

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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