What buffer is this antibody provided in, and what precautions should I take?

This antibody is typically supplied in a PBS (Phosphate-Buffered Saline) solution with a neutral pH. It's crucial to handle the antibody with care to prevent degradation. Avoid repeated freeze-thaw cycles by aliquoting the product into smaller volumes upon receipt. Also, ensure your working buffers and samples do not contain sodium azide if you are using an HRP-conjugated secondary antibody, as sodium azide can inhibit HRP activity.

Can this antibody be used for applications not listed on the datasheet, such as ChIP or ELISA?

While the datasheet lists the validated applications, this antibody may be suitable for other uses like ChIP (Chromatin Immunoprecipitation) or ELISA. Creative Biolabs' policy is to validate their antibodies for a specific set of applications. If you have an unlisted application, it's often best to contact their technical support or request a small trial size to test the antibody's performance in your specific experimental system before purchasing a full-sized vial.

Mouse Anti-ADAM30 Recombinant Antibody (V2-179789) (CBMAB-A1151-YC)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

V2-179789

Application

ELISA

Immunogen

Partial recombinant corresponding to aa199-299 from human ADAM30 (NP_068566) with GST tag.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid in PBS, pH 7.2

Buffer

PBS, pH7.4

Preservative

None

Concentration

Batch dependent

Storage

Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

ADAM metallopeptidase domain 30

Introduction

ADAM30 is a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involvi

Entrez Gene ID

11085

UniProt ID

Q9UKF2

Alternative Names

ADAM Metallopeptidase Domain 30; A Disintegrin And Metalloproteinase Domain 30; Disintegrin And Metalloproteinase Domain-Containing Protein 30; EC 3.4.24.-; ADAM 30; Svph4;

Function

Plays a role in lysosomal amyloid precursor protein (APP) processing by cleaving and activating CTSD/cathepsin D which leads to APP degradation.

Biological Process

Binding of sperm to zona pellucida

Cellular Location

Late endosome membrane

Topology

Extracellular: 199-687 aa
Helical: 688-708 aa
Cytoplasmic: 709-790 aa

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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